Role of Renal Nerves in the Treatment of Renovascular Hypertensive Rats with L-Arginine
The purpose was to determine the role of renal nerves in mediating the effects of antihypertensive treatment with L-arginine in a renovascular hypertension model. The 2K1C (two-kidney one-clip model) hypertensive rats were submitted to bilateral surgical-pharmacological renal denervation. The animal...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Hypertension |
| Online Access: | http://dx.doi.org/10.1155/2014/735627 |
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| author | Sonia Alves Gouvea Renata V. Tiradentes Cintia H. Santuzzi Vinícius Mengal Henrique de A. Futuro Neto Nyam F. Silva Gláucia R. Abreu |
| author_facet | Sonia Alves Gouvea Renata V. Tiradentes Cintia H. Santuzzi Vinícius Mengal Henrique de A. Futuro Neto Nyam F. Silva Gláucia R. Abreu |
| author_sort | Sonia Alves Gouvea |
| collection | DOAJ |
| description | The purpose was to determine the role of renal nerves in mediating the effects of antihypertensive treatment with L-arginine in a renovascular hypertension model. The 2K1C (two-kidney one-clip model) hypertensive rats were submitted to bilateral surgical-pharmacological renal denervation. The animals were subdivided into six experimental groups: normotensive control rats (SHAM), 2K1C rats, 2K1C rats treated with L-arginine (2K1C + L-arg), denervated normotensive (DN) rats, denervated 2K1C (2K1C + DN) rats, and denervated 2K1C + L-arg (2K1C + DN + L-arg) rats. Arterial blood pressure, water intake, urine volume, and sodium excretion were measured. The 2K1C rats exhibited an increase in the mean arterial pressure (MAP) (from 106 ± 3 to 183 ± 5.8 mmHg, P<0.01), whereas L-arg treatment induced a reduction in the MAP (143 ± 3.4 mmHg) without lowering it to the control level. Renal nerve denervation reduced the MAP to normotensive levels in 2K1C rats with or without chronic L-arg treatment. L-arg and denervation induced increases in water intake and urine volume, and L-arg caused a significant natriuretic effect. Our results suggest that renal sympathetic activity participates in the genesis and the maintenance of the hypertension and also demonstrate that treatment with L-arg alone is incapable of normalizing the MAP and that the effect of such treatment is not additive with the effect of kidney denervation. |
| format | Article |
| id | doaj-art-7c56fc3ea1ee49b08ff249c7e216e31e |
| institution | Kabale University |
| issn | 2090-0384 2090-0392 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Hypertension |
| spelling | doaj-art-7c56fc3ea1ee49b08ff249c7e216e31e2025-02-03T06:05:31ZengWileyInternational Journal of Hypertension2090-03842090-03922014-01-01201410.1155/2014/735627735627Role of Renal Nerves in the Treatment of Renovascular Hypertensive Rats with L-ArginineSonia Alves Gouvea0Renata V. Tiradentes1Cintia H. Santuzzi2Vinícius Mengal3Henrique de A. Futuro Neto4Nyam F. Silva5Gláucia R. Abreu6Department of Physiological Sciences, Health Sciences Center, Federal University of Espírito Santo, Avenida Marechal Campos 1456, 29040-577 Vitória, ES, BrazilDepartment of Physiological Sciences, Health Sciences Center, Federal University of Espírito Santo, Avenida Marechal Campos 1456, 29040-577 Vitória, ES, BrazilDepartment of Physiological Sciences, Health Sciences Center, Federal University of Espírito Santo, Avenida Marechal Campos 1456, 29040-577 Vitória, ES, BrazilDepartment of Physiological Sciences, Health Sciences Center, Federal University of Espírito Santo, Avenida Marechal Campos 1456, 29040-577 Vitória, ES, BrazilDepartment of Morphology, Health Sciences Center, Federal University of Espirito Santo, Vitoria, ES, BrazilDepartment of Morphology, Health Sciences Center, Federal University of Espirito Santo, Vitoria, ES, BrazilDepartment of Physiological Sciences, Health Sciences Center, Federal University of Espírito Santo, Avenida Marechal Campos 1456, 29040-577 Vitória, ES, BrazilThe purpose was to determine the role of renal nerves in mediating the effects of antihypertensive treatment with L-arginine in a renovascular hypertension model. The 2K1C (two-kidney one-clip model) hypertensive rats were submitted to bilateral surgical-pharmacological renal denervation. The animals were subdivided into six experimental groups: normotensive control rats (SHAM), 2K1C rats, 2K1C rats treated with L-arginine (2K1C + L-arg), denervated normotensive (DN) rats, denervated 2K1C (2K1C + DN) rats, and denervated 2K1C + L-arg (2K1C + DN + L-arg) rats. Arterial blood pressure, water intake, urine volume, and sodium excretion were measured. The 2K1C rats exhibited an increase in the mean arterial pressure (MAP) (from 106 ± 3 to 183 ± 5.8 mmHg, P<0.01), whereas L-arg treatment induced a reduction in the MAP (143 ± 3.4 mmHg) without lowering it to the control level. Renal nerve denervation reduced the MAP to normotensive levels in 2K1C rats with or without chronic L-arg treatment. L-arg and denervation induced increases in water intake and urine volume, and L-arg caused a significant natriuretic effect. Our results suggest that renal sympathetic activity participates in the genesis and the maintenance of the hypertension and also demonstrate that treatment with L-arg alone is incapable of normalizing the MAP and that the effect of such treatment is not additive with the effect of kidney denervation.http://dx.doi.org/10.1155/2014/735627 |
| spellingShingle | Sonia Alves Gouvea Renata V. Tiradentes Cintia H. Santuzzi Vinícius Mengal Henrique de A. Futuro Neto Nyam F. Silva Gláucia R. Abreu Role of Renal Nerves in the Treatment of Renovascular Hypertensive Rats with L-Arginine International Journal of Hypertension |
| title | Role of Renal Nerves in the Treatment of Renovascular Hypertensive Rats with L-Arginine |
| title_full | Role of Renal Nerves in the Treatment of Renovascular Hypertensive Rats with L-Arginine |
| title_fullStr | Role of Renal Nerves in the Treatment of Renovascular Hypertensive Rats with L-Arginine |
| title_full_unstemmed | Role of Renal Nerves in the Treatment of Renovascular Hypertensive Rats with L-Arginine |
| title_short | Role of Renal Nerves in the Treatment of Renovascular Hypertensive Rats with L-Arginine |
| title_sort | role of renal nerves in the treatment of renovascular hypertensive rats with l arginine |
| url | http://dx.doi.org/10.1155/2014/735627 |
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