Development of a Serogroup B Specific Footrot Vaccine with Montanide ISA 61 VG Adjuvant for Prophylactic and Therapeutic Use in Sheep
Virulent footrot represents as a significant contagious ailment affecting sheep, with Dichelobacter nodosus being the primary agent responsible for transmitting the disease. Vaccinations and various management practices are utilized to control the disease. In this study, we cultured D. nodosus in br...
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Journal of Pure and Applied Microbiology
2025-03-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/development-of-a-serogroup-b-specific-footrot-vaccine-with-montanide-isa-61-vg-adjuvant-for-prophylactic-and-therapeutic-use-in-sheep/ |
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| author | Arham Quraishi Isfaqul Hussain Shaheen Farooq Zahid Amin Kashoo Najeeb Ul Tarfain Sabia Qureshi Mir Nadeem Hassan Mohd Altaf Bhat Mansoor Nabi Mir Syed Mudasir Ahmad Syed Akram Hussain Zahoor Ahmad Wani |
| author_facet | Arham Quraishi Isfaqul Hussain Shaheen Farooq Zahid Amin Kashoo Najeeb Ul Tarfain Sabia Qureshi Mir Nadeem Hassan Mohd Altaf Bhat Mansoor Nabi Mir Syed Mudasir Ahmad Syed Akram Hussain Zahoor Ahmad Wani |
| author_sort | Arham Quraishi |
| collection | DOAJ |
| description | Virulent footrot represents as a significant contagious ailment affecting sheep, with Dichelobacter nodosus being the primary agent responsible for transmitting the disease. Vaccinations and various management practices are utilized to control the disease. In this study, we cultured D. nodosus in broth culture under reducing conditions and formulated a vaccine using Montanide oil as adjuvant. The efficacy of the vaccine was assessed in sheep to determine its ability to elicit an effective immune response and its therapeutic potential. D. nodosus serogroup B (JKS-07B strain) was grown in a modified TAS broth and a whole cell killed vaccine was prepared using 1.5 × 109 cells per dose, along with the oil adjuvant Montanide ISA 61 VG. The vaccine trial was conducted in sheep and two doses of the vaccine were given subcutaneously with a 30 days interval between the doses. The serum antibody titers in the vaccinated animals were measured at 0, 30, 60, 90, 120, and 150 days post-vaccination using the microplate agglutination test. Protective antibody titers (≥ 3000) were achieved by the 30th day following the primary vaccination in all animals, reaching their peak at the 60th day. The protective level of antibody titers was maintained up to 120 days following the primary vaccination. The vaccine also demonstrated a therapeutic effect in animals that were clinically affected. Animals with a lesion score of 2 took 18-21 days for recovery, while those with a lesion score of 3-4 took 40-47 days to recover from lameness. |
| format | Article |
| id | doaj-art-7c5271d197d24f3191dc24832589a2f0 |
| institution | Kabale University |
| issn | 0973-7510 2581-690X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj-art-7c5271d197d24f3191dc24832589a2f02025-08-20T03:42:47ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2025-03-01191255262https://doi.org/10.22207/JPAM.19.1.16Development of a Serogroup B Specific Footrot Vaccine with Montanide ISA 61 VG Adjuvant for Prophylactic and Therapeutic Use in SheepArham QuraishiIsfaqul Hussainhttps://orcid.org/0000-0001-6034-9472Shaheen Farooqhttps://orcid.org/0000-0002-8231-6089Zahid Amin Kashoohttps://orcid.org/0000-0001-6962-7256Najeeb Ul TarfainSabia Qureshihttps://orcid.org/0000-0002-7731-3334Mir Nadeem Hassanhttps://orcid.org/0000-0002-8475-0495Mohd Altaf Bhathttps://orcid.org/0000-0003-0961-4829Mansoor Nabi Mirhttps://orcid.org/0009-0006-2177-6336Syed Mudasir Ahmadhttps://orcid.org/0000-0001-6036-6467Syed Akram Hussainhttps://orcid.org/0000-0002-6041-6169Zahoor Ahmad Wanihttps://orcid.org/0000-0002-1352-0334Virulent footrot represents as a significant contagious ailment affecting sheep, with Dichelobacter nodosus being the primary agent responsible for transmitting the disease. Vaccinations and various management practices are utilized to control the disease. In this study, we cultured D. nodosus in broth culture under reducing conditions and formulated a vaccine using Montanide oil as adjuvant. The efficacy of the vaccine was assessed in sheep to determine its ability to elicit an effective immune response and its therapeutic potential. D. nodosus serogroup B (JKS-07B strain) was grown in a modified TAS broth and a whole cell killed vaccine was prepared using 1.5 × 109 cells per dose, along with the oil adjuvant Montanide ISA 61 VG. The vaccine trial was conducted in sheep and two doses of the vaccine were given subcutaneously with a 30 days interval between the doses. The serum antibody titers in the vaccinated animals were measured at 0, 30, 60, 90, 120, and 150 days post-vaccination using the microplate agglutination test. Protective antibody titers (≥ 3000) were achieved by the 30th day following the primary vaccination in all animals, reaching their peak at the 60th day. The protective level of antibody titers was maintained up to 120 days following the primary vaccination. The vaccine also demonstrated a therapeutic effect in animals that were clinically affected. Animals with a lesion score of 2 took 18-21 days for recovery, while those with a lesion score of 3-4 took 40-47 days to recover from lameness.https://microbiologyjournal.org/development-of-a-serogroup-b-specific-footrot-vaccine-with-montanide-isa-61-vg-adjuvant-for-prophylactic-and-therapeutic-use-in-sheep/dichelobacter nodosusantibody titermontanide isa 61 vgvaccinetherapeutic effect |
| spellingShingle | Arham Quraishi Isfaqul Hussain Shaheen Farooq Zahid Amin Kashoo Najeeb Ul Tarfain Sabia Qureshi Mir Nadeem Hassan Mohd Altaf Bhat Mansoor Nabi Mir Syed Mudasir Ahmad Syed Akram Hussain Zahoor Ahmad Wani Development of a Serogroup B Specific Footrot Vaccine with Montanide ISA 61 VG Adjuvant for Prophylactic and Therapeutic Use in Sheep Journal of Pure and Applied Microbiology dichelobacter nodosus antibody titer montanide isa 61 vg vaccine therapeutic effect |
| title | Development of a Serogroup B Specific Footrot Vaccine with Montanide ISA 61 VG Adjuvant for Prophylactic and Therapeutic Use in Sheep |
| title_full | Development of a Serogroup B Specific Footrot Vaccine with Montanide ISA 61 VG Adjuvant for Prophylactic and Therapeutic Use in Sheep |
| title_fullStr | Development of a Serogroup B Specific Footrot Vaccine with Montanide ISA 61 VG Adjuvant for Prophylactic and Therapeutic Use in Sheep |
| title_full_unstemmed | Development of a Serogroup B Specific Footrot Vaccine with Montanide ISA 61 VG Adjuvant for Prophylactic and Therapeutic Use in Sheep |
| title_short | Development of a Serogroup B Specific Footrot Vaccine with Montanide ISA 61 VG Adjuvant for Prophylactic and Therapeutic Use in Sheep |
| title_sort | development of a serogroup b specific footrot vaccine with montanide isa 61 vg adjuvant for prophylactic and therapeutic use in sheep |
| topic | dichelobacter nodosus antibody titer montanide isa 61 vg vaccine therapeutic effect |
| url | https://microbiologyjournal.org/development-of-a-serogroup-b-specific-footrot-vaccine-with-montanide-isa-61-vg-adjuvant-for-prophylactic-and-therapeutic-use-in-sheep/ |
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