Immunotherapy with Native Molecule rather than Hypoallergenic Variant of Pru p 3, the Major Peach Allergen, Shows Beneficial Effects in Mice
Background. The use of hypoallergenic derivatives is considered beneficial to promote the safety and efficacy of allergen-specific immunotherapy. We aimed to assess the efficacy of reduced and alkylated (R/A) Pru p 3, a hypoallergenic folding variant of the major peach allergen, in subcutaneous immu...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2018/3479185 |
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| author | Maria J. Rodriguez Andrea Wangorsch Francisca Gomez Stefan Schülke Maria J. Torres Stefan Vieths Stephan Scheurer Masako Toda Cristobalina Mayorga |
| author_facet | Maria J. Rodriguez Andrea Wangorsch Francisca Gomez Stefan Schülke Maria J. Torres Stefan Vieths Stephan Scheurer Masako Toda Cristobalina Mayorga |
| author_sort | Maria J. Rodriguez |
| collection | DOAJ |
| description | Background. The use of hypoallergenic derivatives is considered beneficial to promote the safety and efficacy of allergen-specific immunotherapy. We aimed to assess the efficacy of reduced and alkylated (R/A) Pru p 3, a hypoallergenic folding variant of the major peach allergen, in subcutaneous immunotherapy (SCIT) using a murine model of peach allergy. Methods and Results. After sensitization with Pru p 3, BALB/c mice received SCIT with Pru p 3 or R/A Pru p 3 and were challenged with Pru p 3. SCIT with Pru p 3, but not with R/A Pru p 3, suppressed anaphylaxis upon the challenge significantly. SCIT with Pru p 3 did not suppress Pru p 3-specific IgE and IgG1 production, but enhanced IgG2a production. In contrast, SCIT with R/A Pru p 3 suppressed IgE and IgG1 production, but enhanced IgG2a production only moderately. The therapeutic efficacy of SCIT with Pru p 3 was associated with induction of IL-10 and IFN-γ. Conclusion. Hypoallergenic folding variant of Pru p 3 is not likely an efficacious therapeutic component in SCIT of peach allergy. The lower efficacy of R/A Pru p 3 might be attributed to poor antigenicity and/or weak stability due to its unfolded conformation. |
| format | Article |
| id | doaj-art-7c4782f245d647f09680dafa30b5e5ff |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-7c4782f245d647f09680dafa30b5e5ff2025-08-20T03:35:44ZengWileyJournal of Immunology Research2314-88612314-71562018-01-01201810.1155/2018/34791853479185Immunotherapy with Native Molecule rather than Hypoallergenic Variant of Pru p 3, the Major Peach Allergen, Shows Beneficial Effects in MiceMaria J. Rodriguez0Andrea Wangorsch1Francisca Gomez2Stefan Schülke3Maria J. Torres4Stefan Vieths5Stephan Scheurer6Masako Toda7Cristobalina Mayorga8Research Laboratory, IBIMA-Hospital Regional Universitario de Malaga-UMA, Pabellón 5 Sótano, 29009 Malaga, SpainMolecular Allergology, Paul-Ehrlich-Institut, Paul Ehrlich Street 51-59, 63225 Langen, GermanyAllergy Service, IBIMA-Hospital Regional Universitario de Malaga-UMA, Malaga, SpainMolecular Allergology, Paul-Ehrlich-Institut, Paul Ehrlich Street 51-59, 63225 Langen, GermanyAllergy Service, IBIMA-Hospital Regional Universitario de Malaga-UMA, Malaga, SpainMolecular Allergology, Paul-Ehrlich-Institut, Paul Ehrlich Street 51-59, 63225 Langen, GermanyMolecular Allergology, Paul-Ehrlich-Institut, Paul Ehrlich Street 51-59, 63225 Langen, GermanyMolecular Allergology, Paul-Ehrlich-Institut, Paul Ehrlich Street 51-59, 63225 Langen, GermanyResearch Laboratory, IBIMA-Hospital Regional Universitario de Malaga-UMA, Pabellón 5 Sótano, 29009 Malaga, SpainBackground. The use of hypoallergenic derivatives is considered beneficial to promote the safety and efficacy of allergen-specific immunotherapy. We aimed to assess the efficacy of reduced and alkylated (R/A) Pru p 3, a hypoallergenic folding variant of the major peach allergen, in subcutaneous immunotherapy (SCIT) using a murine model of peach allergy. Methods and Results. After sensitization with Pru p 3, BALB/c mice received SCIT with Pru p 3 or R/A Pru p 3 and were challenged with Pru p 3. SCIT with Pru p 3, but not with R/A Pru p 3, suppressed anaphylaxis upon the challenge significantly. SCIT with Pru p 3 did not suppress Pru p 3-specific IgE and IgG1 production, but enhanced IgG2a production. In contrast, SCIT with R/A Pru p 3 suppressed IgE and IgG1 production, but enhanced IgG2a production only moderately. The therapeutic efficacy of SCIT with Pru p 3 was associated with induction of IL-10 and IFN-γ. Conclusion. Hypoallergenic folding variant of Pru p 3 is not likely an efficacious therapeutic component in SCIT of peach allergy. The lower efficacy of R/A Pru p 3 might be attributed to poor antigenicity and/or weak stability due to its unfolded conformation.http://dx.doi.org/10.1155/2018/3479185 |
| spellingShingle | Maria J. Rodriguez Andrea Wangorsch Francisca Gomez Stefan Schülke Maria J. Torres Stefan Vieths Stephan Scheurer Masako Toda Cristobalina Mayorga Immunotherapy with Native Molecule rather than Hypoallergenic Variant of Pru p 3, the Major Peach Allergen, Shows Beneficial Effects in Mice Journal of Immunology Research |
| title | Immunotherapy with Native Molecule rather than Hypoallergenic Variant of Pru p 3, the Major Peach Allergen, Shows Beneficial Effects in Mice |
| title_full | Immunotherapy with Native Molecule rather than Hypoallergenic Variant of Pru p 3, the Major Peach Allergen, Shows Beneficial Effects in Mice |
| title_fullStr | Immunotherapy with Native Molecule rather than Hypoallergenic Variant of Pru p 3, the Major Peach Allergen, Shows Beneficial Effects in Mice |
| title_full_unstemmed | Immunotherapy with Native Molecule rather than Hypoallergenic Variant of Pru p 3, the Major Peach Allergen, Shows Beneficial Effects in Mice |
| title_short | Immunotherapy with Native Molecule rather than Hypoallergenic Variant of Pru p 3, the Major Peach Allergen, Shows Beneficial Effects in Mice |
| title_sort | immunotherapy with native molecule rather than hypoallergenic variant of pru p 3 the major peach allergen shows beneficial effects in mice |
| url | http://dx.doi.org/10.1155/2018/3479185 |
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