Evaluation of Morphological and Functional Nerve Recovery of Rat Sciatic Nerve with a Hyaff11-Based Nerve Guide
Application of a Hyaff11-based nerve guide was studied in rats. Functional tests were performed to study motor nerve recovery. A withdrawal reflex test was performed to test sensory recovery. Morphology was studied by means of histology on explanted tissue samples. Motor nerve recovery was establish...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2006-01-01
|
| Series: | Applied Bionics and Biomechanics |
| Online Access: | http://dx.doi.org/10.1533/abbi.2005.0017 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850220799992004608 |
|---|---|
| author | K. Jansen Y. Ludwig M. J. A. van Luyn A. A. Gramsbergen M. F. Meek |
| author_facet | K. Jansen Y. Ludwig M. J. A. van Luyn A. A. Gramsbergen M. F. Meek |
| author_sort | K. Jansen |
| collection | DOAJ |
| description | Application of a Hyaff11-based nerve guide was studied in rats. Functional tests were performed to study motor nerve recovery. A withdrawal reflex test was performed to test sensory recovery. Morphology was studied by means of histology on explanted tissue samples. Motor nerve recovery was established within 7 weeks. Hereafter, some behavioral parameters like alternating steps showed an increase in occurence, while others remained stable. Sensory function was observed within the 7 weeks time frame. Nerve tissue had bridged the 10-mm gap within 7 weeks. The average nerve fiber surface area increased significantly in time. In situ degradation of the nerve conduit was fully going on at week 7 and tubes had collapsed by then. At weeks 15 and 21, the knitted tube wall structure was completely surrounded by macrophages and giant cells, and matrix was penetrating the tube wall. We conclude that a Hyaff11-based nerve guide can be used to bridge short peripheral nerve defects in rat. However, adaptations need to be made. |
| format | Article |
| id | doaj-art-7c46da363d9649cba7cb4aa5ab4e1d8a |
| institution | OA Journals |
| issn | 1176-2322 1754-2103 |
| language | English |
| publishDate | 2006-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applied Bionics and Biomechanics |
| spelling | doaj-art-7c46da363d9649cba7cb4aa5ab4e1d8a2025-08-20T02:06:55ZengWileyApplied Bionics and Biomechanics1176-23221754-21032006-01-0132677510.1533/abbi.2005.0017Evaluation of Morphological and Functional Nerve Recovery of Rat Sciatic Nerve with a Hyaff11-Based Nerve GuideK. Jansen0Y. Ludwig1M. J. A. van Luyn2A. A. Gramsbergen3M. F. Meek4Hutchinson Technology Inc., IJsselburcht 3, Arnhem, NetherlandsTissue Engineering, Medical Biology, Department of Pathology Lab Medicine, Faculty of Medical Sciences, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, NetherlandsTissue Engineering, Medical Biology, Department of Pathology Lab Medicine, Faculty of Medical Sciences, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, NetherlandsDepartment of Medical Physiology, University of Groningen, A. Deusinglaan 1, 9713 AV, Groningen, NetherlandsTissue Engineering, Medical Biology, Department of Pathology Lab Medicine, Faculty of Medical Sciences, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, NetherlandsApplication of a Hyaff11-based nerve guide was studied in rats. Functional tests were performed to study motor nerve recovery. A withdrawal reflex test was performed to test sensory recovery. Morphology was studied by means of histology on explanted tissue samples. Motor nerve recovery was established within 7 weeks. Hereafter, some behavioral parameters like alternating steps showed an increase in occurence, while others remained stable. Sensory function was observed within the 7 weeks time frame. Nerve tissue had bridged the 10-mm gap within 7 weeks. The average nerve fiber surface area increased significantly in time. In situ degradation of the nerve conduit was fully going on at week 7 and tubes had collapsed by then. At weeks 15 and 21, the knitted tube wall structure was completely surrounded by macrophages and giant cells, and matrix was penetrating the tube wall. We conclude that a Hyaff11-based nerve guide can be used to bridge short peripheral nerve defects in rat. However, adaptations need to be made.http://dx.doi.org/10.1533/abbi.2005.0017 |
| spellingShingle | K. Jansen Y. Ludwig M. J. A. van Luyn A. A. Gramsbergen M. F. Meek Evaluation of Morphological and Functional Nerve Recovery of Rat Sciatic Nerve with a Hyaff11-Based Nerve Guide Applied Bionics and Biomechanics |
| title | Evaluation of Morphological and Functional Nerve Recovery of Rat Sciatic Nerve with a Hyaff11-Based Nerve Guide |
| title_full | Evaluation of Morphological and Functional Nerve Recovery of Rat Sciatic Nerve with a Hyaff11-Based Nerve Guide |
| title_fullStr | Evaluation of Morphological and Functional Nerve Recovery of Rat Sciatic Nerve with a Hyaff11-Based Nerve Guide |
| title_full_unstemmed | Evaluation of Morphological and Functional Nerve Recovery of Rat Sciatic Nerve with a Hyaff11-Based Nerve Guide |
| title_short | Evaluation of Morphological and Functional Nerve Recovery of Rat Sciatic Nerve with a Hyaff11-Based Nerve Guide |
| title_sort | evaluation of morphological and functional nerve recovery of rat sciatic nerve with a hyaff11 based nerve guide |
| url | http://dx.doi.org/10.1533/abbi.2005.0017 |
| work_keys_str_mv | AT kjansen evaluationofmorphologicalandfunctionalnerverecoveryofratsciaticnervewithahyaff11basednerveguide AT yludwig evaluationofmorphologicalandfunctionalnerverecoveryofratsciaticnervewithahyaff11basednerveguide AT mjavanluyn evaluationofmorphologicalandfunctionalnerverecoveryofratsciaticnervewithahyaff11basednerveguide AT aagramsbergen evaluationofmorphologicalandfunctionalnerverecoveryofratsciaticnervewithahyaff11basednerveguide AT mfmeek evaluationofmorphologicalandfunctionalnerverecoveryofratsciaticnervewithahyaff11basednerveguide |