Screening Activities of Crude Extracts Produced Halodule sp. Seagrass-Associated Fungus
Secondary metabolites from marine microorganisms, including marine-derived fungi, have consented to developing guide bioactive compounds. Marine-derived fungi were reported to be associated with various habitats, including seagrasses. The seagrass-associated fungus from the Indonesian marine area is...
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Diponegoro University; Association of Indonesian Coastal Management Experts
2025-06-01
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| Series: | Ilmu Kelautan |
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| Online Access: | https://ejournal.undip.ac.id/index.php/ijms/article/view/49706 |
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| author | Safwan Safwan Sucilawaty Ridwan Musyarrafah Musyarrafah Rina Wijayanti |
| author_facet | Safwan Safwan Sucilawaty Ridwan Musyarrafah Musyarrafah Rina Wijayanti |
| author_sort | Safwan Safwan |
| collection | DOAJ |
| description | Secondary metabolites from marine microorganisms, including marine-derived fungi, have consented to developing guide bioactive compounds. Marine-derived fungi were reported to be associated with various habitats, including seagrasses. The seagrass-associated fungus from the Indonesian marine area is still poorly unexplored. This study was presented to screen for the antimicrobial and antioxidant activity of crude extracts produced by fungi associated with Seagrass (Halodule sp.) collected from Indonesia. Fresh samples were collected and kept fresh until they arrived in the Laboratory and immediately planted on Potato Dextrose Agar (PDA) Sea Salt media. Ten fungi were isolated and purified using a Malt Extract Agar (MEA) medium and subjected to fermentation treatment for 30 days using solid rice media. The compounds produced were collected by soaking directly using ethyl acetate (EA) for one hour while stirring mechanically. Evaporation of EA was carried out to obtain crude extracts. Each crude extract was subjected to antibacterial and antioxidant activities using the agar diffusion and DPPH methods, respectively. Reverse-phase Thin-Layer Chromatography (TLC) was used to observe the compound profile. The results of the activity test showed that two crude extracts from fungi with the code A.10.4 and B.5.1 had both activities, antibacterial (potent inhibition at 50% concentration and antioxidant (IC50 of 90.23 and 88.29 ppm, respectively). Another crude extract with the fungi code B.1.1 showed strong antioxidant activity (IC50 of 81.31 ppm) without antimicrobial activity. TLC results show different compound profiles from each crude extract and quite a good separation. |
| format | Article |
| id | doaj-art-7c291c78f0a945869fa4d1cb692e3f33 |
| institution | DOAJ |
| issn | 0853-7291 2406-7598 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Diponegoro University; Association of Indonesian Coastal Management Experts |
| record_format | Article |
| series | Ilmu Kelautan |
| spelling | doaj-art-7c291c78f0a945869fa4d1cb692e3f332025-08-20T03:15:02ZengDiponegoro University; Association of Indonesian Coastal Management ExpertsIlmu Kelautan0853-72912406-75982025-06-0130223724410.14710/ik.ijms.30.2.237-24426028Screening Activities of Crude Extracts Produced Halodule sp. Seagrass-Associated FungusSafwan Safwan0https://orcid.org/0000-0002-8048-0301Sucilawaty Ridwan1Musyarrafah Musyarrafah2Rina Wijayanti3Department of Pharmacy, University of Muhammadiyah Mataram , IndonesiaDepartment of Pharmacy, Faculty of Medicine, Universitas Mataram, IndonesiaDepartment of Biochemistry, Faculty of Medical , Universitas Islam Al-Azhar, IndonesiaDepartment of Pharmacy, Faculty of Medical, Sultan Agung Islamic University, IndonesiaSecondary metabolites from marine microorganisms, including marine-derived fungi, have consented to developing guide bioactive compounds. Marine-derived fungi were reported to be associated with various habitats, including seagrasses. The seagrass-associated fungus from the Indonesian marine area is still poorly unexplored. This study was presented to screen for the antimicrobial and antioxidant activity of crude extracts produced by fungi associated with Seagrass (Halodule sp.) collected from Indonesia. Fresh samples were collected and kept fresh until they arrived in the Laboratory and immediately planted on Potato Dextrose Agar (PDA) Sea Salt media. Ten fungi were isolated and purified using a Malt Extract Agar (MEA) medium and subjected to fermentation treatment for 30 days using solid rice media. The compounds produced were collected by soaking directly using ethyl acetate (EA) for one hour while stirring mechanically. Evaporation of EA was carried out to obtain crude extracts. Each crude extract was subjected to antibacterial and antioxidant activities using the agar diffusion and DPPH methods, respectively. Reverse-phase Thin-Layer Chromatography (TLC) was used to observe the compound profile. The results of the activity test showed that two crude extracts from fungi with the code A.10.4 and B.5.1 had both activities, antibacterial (potent inhibition at 50% concentration and antioxidant (IC50 of 90.23 and 88.29 ppm, respectively). Another crude extract with the fungi code B.1.1 showed strong antioxidant activity (IC50 of 81.31 ppm) without antimicrobial activity. TLC results show different compound profiles from each crude extract and quite a good separation.https://ejournal.undip.ac.id/index.php/ijms/article/view/49706fungiantimicrobialantioxidantmarineseagrasshalodule sp. |
| spellingShingle | Safwan Safwan Sucilawaty Ridwan Musyarrafah Musyarrafah Rina Wijayanti Screening Activities of Crude Extracts Produced Halodule sp. Seagrass-Associated Fungus Ilmu Kelautan fungi antimicrobial antioxidant marine seagrass halodule sp. |
| title | Screening Activities of Crude Extracts Produced Halodule sp. Seagrass-Associated Fungus |
| title_full | Screening Activities of Crude Extracts Produced Halodule sp. Seagrass-Associated Fungus |
| title_fullStr | Screening Activities of Crude Extracts Produced Halodule sp. Seagrass-Associated Fungus |
| title_full_unstemmed | Screening Activities of Crude Extracts Produced Halodule sp. Seagrass-Associated Fungus |
| title_short | Screening Activities of Crude Extracts Produced Halodule sp. Seagrass-Associated Fungus |
| title_sort | screening activities of crude extracts produced halodule sp seagrass associated fungus |
| topic | fungi antimicrobial antioxidant marine seagrass halodule sp. |
| url | https://ejournal.undip.ac.id/index.php/ijms/article/view/49706 |
| work_keys_str_mv | AT safwansafwan screeningactivitiesofcrudeextractsproducedhalodulespseagrassassociatedfungus AT sucilawatyridwan screeningactivitiesofcrudeextractsproducedhalodulespseagrassassociatedfungus AT musyarrafahmusyarrafah screeningactivitiesofcrudeextractsproducedhalodulespseagrassassociatedfungus AT rinawijayanti screeningactivitiesofcrudeextractsproducedhalodulespseagrassassociatedfungus |