Deletion of Snai2 and Snai3 results in impaired physical development compounded by lymphocyte deficiency.
The Snail family of transcriptional regulators consists of three highly conserved members. These proteins regulate (repress) transcription via the recruitment of histone deacetylases to target gene promoters that possess the appropriate E-box binding sequences. Murine Snai1 is required for mouse dev...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0069216 |
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| author | Peter D Pioli Timothy J Dahlem Janis J Weis John H Weis |
| author_facet | Peter D Pioli Timothy J Dahlem Janis J Weis John H Weis |
| author_sort | Peter D Pioli |
| collection | DOAJ |
| description | The Snail family of transcriptional regulators consists of three highly conserved members. These proteins regulate (repress) transcription via the recruitment of histone deacetylases to target gene promoters that possess the appropriate E-box binding sequences. Murine Snai1 is required for mouse development while Snai2 deficient animals survive with some anomalies. Less is known about the third member of the family, Snai3. To investigate the function of Snai3, we generated a conditional knockin mouse. Utilizing Cre-mediated deletion to facilitate the ablation of Snai3 in T cells or the entire animal, we found little to no effect of the loss of Snai3 in the entire animal or in T cell lineages. This finding provided the hypothesis that absence of Snai3 was mitigated, in part, by the presence of Snai2. To test this hypothesis we created Snai2/Snai3 double deficient mice. The developmental consequences of lacking both of these proteins was manifested in stunted growth, a paucity of offspring including a dramatic deficiency of female mice, and impaired immune cell development within the lymphoid lineages. |
| format | Article |
| id | doaj-art-7c0b699ca2a6488995df4e44e7c4da98 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-7c0b699ca2a6488995df4e44e7c4da982025-08-20T02:33:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0187e6921610.1371/journal.pone.0069216Deletion of Snai2 and Snai3 results in impaired physical development compounded by lymphocyte deficiency.Peter D PioliTimothy J DahlemJanis J WeisJohn H WeisThe Snail family of transcriptional regulators consists of three highly conserved members. These proteins regulate (repress) transcription via the recruitment of histone deacetylases to target gene promoters that possess the appropriate E-box binding sequences. Murine Snai1 is required for mouse development while Snai2 deficient animals survive with some anomalies. Less is known about the third member of the family, Snai3. To investigate the function of Snai3, we generated a conditional knockin mouse. Utilizing Cre-mediated deletion to facilitate the ablation of Snai3 in T cells or the entire animal, we found little to no effect of the loss of Snai3 in the entire animal or in T cell lineages. This finding provided the hypothesis that absence of Snai3 was mitigated, in part, by the presence of Snai2. To test this hypothesis we created Snai2/Snai3 double deficient mice. The developmental consequences of lacking both of these proteins was manifested in stunted growth, a paucity of offspring including a dramatic deficiency of female mice, and impaired immune cell development within the lymphoid lineages.https://doi.org/10.1371/journal.pone.0069216 |
| spellingShingle | Peter D Pioli Timothy J Dahlem Janis J Weis John H Weis Deletion of Snai2 and Snai3 results in impaired physical development compounded by lymphocyte deficiency. PLoS ONE |
| title | Deletion of Snai2 and Snai3 results in impaired physical development compounded by lymphocyte deficiency. |
| title_full | Deletion of Snai2 and Snai3 results in impaired physical development compounded by lymphocyte deficiency. |
| title_fullStr | Deletion of Snai2 and Snai3 results in impaired physical development compounded by lymphocyte deficiency. |
| title_full_unstemmed | Deletion of Snai2 and Snai3 results in impaired physical development compounded by lymphocyte deficiency. |
| title_short | Deletion of Snai2 and Snai3 results in impaired physical development compounded by lymphocyte deficiency. |
| title_sort | deletion of snai2 and snai3 results in impaired physical development compounded by lymphocyte deficiency |
| url | https://doi.org/10.1371/journal.pone.0069216 |
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