The genetic feature and virulence determinant of highly virulent community-associated MRSA ST338-SCCmec Vb in China
ST59 is the predominant pathotype of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) in China. As a variant of ST59, there is relatively little known about the detailed information of ST338. To address this issue, here, we described thirteen ST338 CA-MRSA strains isolated...
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Taylor & Francis Group
2021-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2021.1914516 |
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| author | Ye Jin Wangxiao Zhou Zhidong Yin Shuntian Zhang Yunbo Chen Ping Shen Jinru Ji Weiwei Chen Beiwen Zheng Yonghong Xiao |
| author_facet | Ye Jin Wangxiao Zhou Zhidong Yin Shuntian Zhang Yunbo Chen Ping Shen Jinru Ji Weiwei Chen Beiwen Zheng Yonghong Xiao |
| author_sort | Ye Jin |
| collection | DOAJ |
| description | ST59 is the predominant pathotype of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) in China. As a variant of ST59, there is relatively little known about the detailed information of ST338. To address this issue, here, we described thirteen ST338 CA-MRSA strains isolated from severe bloodstream infection cases, and focused on their epidemiology, genetic features and virulence potential. Phylogenetic analysis showed the earliest isolated strain of this study is likely a predecessor of recent ST338 lineage (after year of 2014). Furthermore, the phylogenetic reconstruction and time estimation suggested that ST338 evolved from ST59 in 1991. Notably, the carrying patten of virulence factors of all ST338 strains were similar, and the genomic islands νSaα, νSaγ and SaPI and the core virulence factors like hla and psm were detected in ST338 isolates. However, all ST338 isolates lacked some adhesion factors such as clfA, clfB, eap, cna and icaD. Additionally, among these ST338 strains, one PVL-negative ST338 isolate was detected. Experiment on mice nose and human alveolar epithelial cell showed that the nasal colonization ability of ST338 was weaker than that of CA-MRSA MW2. In a mouse bloodstream infection model and skin infection model, PVL+ and PVL− strains had the similar virulence, which was dependent on upregulation of toxin genes rather than the presence of mobile genetic elements such as ΦSa2 carrying PVL. Our findings provide important insight into the epidemiology and pathogenicity of the novel and highly virulent ST338-SCCmec Vb clone. |
| format | Article |
| id | doaj-art-7c0af66e69d844dbbcda0f2fec44ec6f |
| institution | Kabale University |
| issn | 2222-1751 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-7c0af66e69d844dbbcda0f2fec44ec6f2025-08-20T03:52:56ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512021-01-011011052106410.1080/22221751.2021.1914516The genetic feature and virulence determinant of highly virulent community-associated MRSA ST338-SCCmec Vb in ChinaYe Jin0Wangxiao Zhou1Zhidong Yin2Shuntian Zhang3Yunbo Chen4Ping Shen5Jinru Ji6Weiwei Chen7Beiwen Zheng8Yonghong Xiao9State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, People’s Republic of ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, People’s Republic of ChinaDepartment of Pathology, The Second Affiliated Hospital of Zhejiang University College of Medicine, Hangzhou, People’s Republic of ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, People’s Republic of ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, People’s Republic of ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, People’s Republic of ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, People’s Republic of ChinaDepartment of Laboratory Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, People’s Republic of ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, People’s Republic of ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, People’s Republic of ChinaST59 is the predominant pathotype of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) in China. As a variant of ST59, there is relatively little known about the detailed information of ST338. To address this issue, here, we described thirteen ST338 CA-MRSA strains isolated from severe bloodstream infection cases, and focused on their epidemiology, genetic features and virulence potential. Phylogenetic analysis showed the earliest isolated strain of this study is likely a predecessor of recent ST338 lineage (after year of 2014). Furthermore, the phylogenetic reconstruction and time estimation suggested that ST338 evolved from ST59 in 1991. Notably, the carrying patten of virulence factors of all ST338 strains were similar, and the genomic islands νSaα, νSaγ and SaPI and the core virulence factors like hla and psm were detected in ST338 isolates. However, all ST338 isolates lacked some adhesion factors such as clfA, clfB, eap, cna and icaD. Additionally, among these ST338 strains, one PVL-negative ST338 isolate was detected. Experiment on mice nose and human alveolar epithelial cell showed that the nasal colonization ability of ST338 was weaker than that of CA-MRSA MW2. In a mouse bloodstream infection model and skin infection model, PVL+ and PVL− strains had the similar virulence, which was dependent on upregulation of toxin genes rather than the presence of mobile genetic elements such as ΦSa2 carrying PVL. Our findings provide important insight into the epidemiology and pathogenicity of the novel and highly virulent ST338-SCCmec Vb clone.https://www.tandfonline.com/doi/10.1080/22221751.2021.1914516CA-MRSAbloodstream infectionPanton–Valentine leucocidincore-virulence factorsST338 |
| spellingShingle | Ye Jin Wangxiao Zhou Zhidong Yin Shuntian Zhang Yunbo Chen Ping Shen Jinru Ji Weiwei Chen Beiwen Zheng Yonghong Xiao The genetic feature and virulence determinant of highly virulent community-associated MRSA ST338-SCCmec Vb in China Emerging Microbes and Infections CA-MRSA bloodstream infection Panton–Valentine leucocidin core-virulence factors ST338 |
| title | The genetic feature and virulence determinant of highly virulent community-associated MRSA ST338-SCCmec Vb in China |
| title_full | The genetic feature and virulence determinant of highly virulent community-associated MRSA ST338-SCCmec Vb in China |
| title_fullStr | The genetic feature and virulence determinant of highly virulent community-associated MRSA ST338-SCCmec Vb in China |
| title_full_unstemmed | The genetic feature and virulence determinant of highly virulent community-associated MRSA ST338-SCCmec Vb in China |
| title_short | The genetic feature and virulence determinant of highly virulent community-associated MRSA ST338-SCCmec Vb in China |
| title_sort | genetic feature and virulence determinant of highly virulent community associated mrsa st338 sccmec vb in china |
| topic | CA-MRSA bloodstream infection Panton–Valentine leucocidin core-virulence factors ST338 |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2021.1914516 |
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