Development and characterization of a novel injectable thyroid extracellular matrix hydrogel for enhanced thyroid tissue engineering applications
Hypothyroidism, a condition characterized by decreased synthesis and secretion of thyroid hormones, significantly impacts intellectual development and physical growth. Current treatments, including hormone replacement therapy and thyroid transplantation, have limitations due to issues like hormone d...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1481295/full |
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| author | Liang Zhang Liang Zhang Houlong Long Houlong Long Peng Zhang Peng Zhang Bin Liu Bin Liu Shuheng Li Shuheng Li Rong Sun Rong Sun Tongmei Diao Tongmei Diao Feng Li Feng Li |
| author_facet | Liang Zhang Liang Zhang Houlong Long Houlong Long Peng Zhang Peng Zhang Bin Liu Bin Liu Shuheng Li Shuheng Li Rong Sun Rong Sun Tongmei Diao Tongmei Diao Feng Li Feng Li |
| author_sort | Liang Zhang |
| collection | DOAJ |
| description | Hypothyroidism, a condition characterized by decreased synthesis and secretion of thyroid hormones, significantly impacts intellectual development and physical growth. Current treatments, including hormone replacement therapy and thyroid transplantation, have limitations due to issues like hormone dosage control and immune rejection. Tissue engineering presents a potential solution by combining cells and biomaterials to construct engineered thyroid tissue. This study focuses on the development and characterization of a novel 3D injectable hydrogel derived from thyroid extracellular matrix (TEM) for thyroid tissue engineering. TEM hydrogels were prepared through decellularization of rat thyroid tissue, followed by extensive physicochemical and mechanical property evaluations. The TEM hydrogels exhibited properties similar to natural thyroid tissue, including high biocompatibility and a complex 3D ultrastructure. Thyroid hormone-secreting cells cultured in TEM hydrogels demonstrated superior viability, hormone secretion, and thyroid-related gene expression compared to those in traditional type I collagen hydrogels. The study also confirmed the significant retention of key growth factors and ECM proteins within the TEM hydrogels. The results indicate that TEM hydrogels can provide a biomimetic microenvironment, promoting the long-term survival and function of thyroid cells, thus holding great promise for the treatment of hypothyroidism. This research contributes a potential new avenue for thyroid tissue engineering, offering a promising alternative for hypothyroidism treatment. |
| format | Article |
| id | doaj-art-7be8240fe9744b88acb89d592be42df3 |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-7be8240fe9744b88acb89d592be42df32024-11-27T04:26:24ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852024-11-011210.3389/fbioe.2024.14812951481295Development and characterization of a novel injectable thyroid extracellular matrix hydrogel for enhanced thyroid tissue engineering applicationsLiang Zhang0Liang Zhang1Houlong Long2Houlong Long3Peng Zhang4Peng Zhang5Bin Liu6Bin Liu7Shuheng Li8Shuheng Li9Rong Sun10Rong Sun11Tongmei Diao12Tongmei Diao13Feng Li14Feng Li15Department of Thyroid and Breast Surgery, Tengzhou Hospital Affiliated to Xuzhou Medical University, Tengzhou, ChinaDepartment of Thyroid and Breast Surgery, Tengzhou City Center People’s Hospital, Zaozhuang, Shandong, ChinaDepartment of Thyroid and Breast Surgery, Tengzhou Hospital Affiliated to Xuzhou Medical University, Tengzhou, ChinaDepartment of Thyroid and Breast Surgery, Tengzhou City Center People’s Hospital, Zaozhuang, Shandong, ChinaDepartment of Thyroid and Breast Surgery, Tengzhou Hospital Affiliated to Xuzhou Medical University, Tengzhou, ChinaDepartment of Thyroid and Breast Surgery, Tengzhou City Center People’s Hospital, Zaozhuang, Shandong, ChinaDepartment of Thyroid and Breast Surgery, Tengzhou Hospital Affiliated to Xuzhou Medical University, Tengzhou, ChinaDepartment of Thyroid and Breast Surgery, Tengzhou City Center People’s Hospital, Zaozhuang, Shandong, ChinaDepartment of Thyroid and Breast Surgery, Tengzhou Hospital Affiliated to Xuzhou Medical University, Tengzhou, ChinaDepartment of Thyroid and Breast Surgery, Tengzhou City Center People’s Hospital, Zaozhuang, Shandong, ChinaDepartment of Thyroid and Breast Surgery, Tengzhou Hospital Affiliated to Xuzhou Medical University, Tengzhou, ChinaDepartment of Thyroid and Breast Surgery, Tengzhou City Center People’s Hospital, Zaozhuang, Shandong, ChinaDepartment of Thyroid and Breast Surgery, Tengzhou Hospital Affiliated to Xuzhou Medical University, Tengzhou, ChinaDepartment of Thyroid and Breast Surgery, Tengzhou City Center People’s Hospital, Zaozhuang, Shandong, ChinaDepartment of Thyroid and Breast Surgery, Tengzhou Hospital Affiliated to Xuzhou Medical University, Tengzhou, ChinaDepartment of Thyroid and Breast Surgery, Tengzhou City Center People’s Hospital, Zaozhuang, Shandong, ChinaHypothyroidism, a condition characterized by decreased synthesis and secretion of thyroid hormones, significantly impacts intellectual development and physical growth. Current treatments, including hormone replacement therapy and thyroid transplantation, have limitations due to issues like hormone dosage control and immune rejection. Tissue engineering presents a potential solution by combining cells and biomaterials to construct engineered thyroid tissue. This study focuses on the development and characterization of a novel 3D injectable hydrogel derived from thyroid extracellular matrix (TEM) for thyroid tissue engineering. TEM hydrogels were prepared through decellularization of rat thyroid tissue, followed by extensive physicochemical and mechanical property evaluations. The TEM hydrogels exhibited properties similar to natural thyroid tissue, including high biocompatibility and a complex 3D ultrastructure. Thyroid hormone-secreting cells cultured in TEM hydrogels demonstrated superior viability, hormone secretion, and thyroid-related gene expression compared to those in traditional type I collagen hydrogels. The study also confirmed the significant retention of key growth factors and ECM proteins within the TEM hydrogels. The results indicate that TEM hydrogels can provide a biomimetic microenvironment, promoting the long-term survival and function of thyroid cells, thus holding great promise for the treatment of hypothyroidism. This research contributes a potential new avenue for thyroid tissue engineering, offering a promising alternative for hypothyroidism treatment.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1481295/fullhypothyroidismthyroid extracellular matrixinjectable hydrogelcell viabilitythyroid hormone secretionthyroid tissue engineering |
| spellingShingle | Liang Zhang Liang Zhang Houlong Long Houlong Long Peng Zhang Peng Zhang Bin Liu Bin Liu Shuheng Li Shuheng Li Rong Sun Rong Sun Tongmei Diao Tongmei Diao Feng Li Feng Li Development and characterization of a novel injectable thyroid extracellular matrix hydrogel for enhanced thyroid tissue engineering applications Frontiers in Bioengineering and Biotechnology hypothyroidism thyroid extracellular matrix injectable hydrogel cell viability thyroid hormone secretion thyroid tissue engineering |
| title | Development and characterization of a novel injectable thyroid extracellular matrix hydrogel for enhanced thyroid tissue engineering applications |
| title_full | Development and characterization of a novel injectable thyroid extracellular matrix hydrogel for enhanced thyroid tissue engineering applications |
| title_fullStr | Development and characterization of a novel injectable thyroid extracellular matrix hydrogel for enhanced thyroid tissue engineering applications |
| title_full_unstemmed | Development and characterization of a novel injectable thyroid extracellular matrix hydrogel for enhanced thyroid tissue engineering applications |
| title_short | Development and characterization of a novel injectable thyroid extracellular matrix hydrogel for enhanced thyroid tissue engineering applications |
| title_sort | development and characterization of a novel injectable thyroid extracellular matrix hydrogel for enhanced thyroid tissue engineering applications |
| topic | hypothyroidism thyroid extracellular matrix injectable hydrogel cell viability thyroid hormone secretion thyroid tissue engineering |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1481295/full |
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