Resistance Modulation Action, Time-Kill Kinetics Assay, and Inhibition of Biofilm Formation Effects of Plumbagin from Plumbago zeylanica Linn
Antimicrobial resistance (AMR) is a threat to the prevention and treatment of the increasing range of infectious diseases. There is therefore the need for renewed efforts into antimicrobial discovery and development to combat the menace. The antimicrobial activity of plumbagin isolated from roots of...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Journal of Tropical Medicine |
| Online Access: | http://dx.doi.org/10.1155/2019/1250645 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832566829540704256 |
|---|---|
| author | Emmanuel B. A. Adusei Reimmel K. Adosraku James Oppong-Kyekyeku Cedric D. K. Amengor Yakubu Jibira |
| author_facet | Emmanuel B. A. Adusei Reimmel K. Adosraku James Oppong-Kyekyeku Cedric D. K. Amengor Yakubu Jibira |
| author_sort | Emmanuel B. A. Adusei |
| collection | DOAJ |
| description | Antimicrobial resistance (AMR) is a threat to the prevention and treatment of the increasing range of infectious diseases. There is therefore the need for renewed efforts into antimicrobial discovery and development to combat the menace. The antimicrobial activity of plumbagin isolated from roots of Plumbago zeylanica against selected organisms was evaluated for resistance modulation antimicrobial assay, time-kill kinetics assay, and inhibition of biofilm formation. The minimum inhibitory concentrations (MICs) of plumbagin and standard drugs were determined via the broth microdilution method to be 0.5 to 8 μg/mL and 0.25–128 μg/mL, respectively. In the resistance modulation study, MICs of the standard drugs were redetermined in the presence of subinhibitory concentration of plumbagin (4 μg/mL), and plumbagin was found to either potentiate or reduce the activities of these standard drugs with the highest potentiation recorded up to 12-folds for ketoconazole against Candida albicans. Plumbagin was found to be bacteriostatic and fungistatic from the time-kill kinetics study. Plumbagin demonstrated strong inhibition of biofilm formation activity at concentrations of 128, 64, and 32 μg/mL against the test microorganisms compared with ciprofloxacin. Plumbagin has been proved through this study to be a suitable lead compound in antimicrobial resistance drug development. |
| format | Article |
| id | doaj-art-7bd0ac19e5af4b6699063770fecc7b6d |
| institution | Kabale University |
| issn | 1687-9686 1687-9694 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Tropical Medicine |
| spelling | doaj-art-7bd0ac19e5af4b6699063770fecc7b6d2025-02-03T01:03:07ZengWileyJournal of Tropical Medicine1687-96861687-96942019-01-01201910.1155/2019/12506451250645Resistance Modulation Action, Time-Kill Kinetics Assay, and Inhibition of Biofilm Formation Effects of Plumbagin from Plumbago zeylanica LinnEmmanuel B. A. Adusei0Reimmel K. Adosraku1James Oppong-Kyekyeku2Cedric D. K. Amengor3Yakubu Jibira4Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Pharmaceutical Chemistry, School of Pharmacy, University of Health and Allied Sciences, Ho, GhanaDepartment of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaAntimicrobial resistance (AMR) is a threat to the prevention and treatment of the increasing range of infectious diseases. There is therefore the need for renewed efforts into antimicrobial discovery and development to combat the menace. The antimicrobial activity of plumbagin isolated from roots of Plumbago zeylanica against selected organisms was evaluated for resistance modulation antimicrobial assay, time-kill kinetics assay, and inhibition of biofilm formation. The minimum inhibitory concentrations (MICs) of plumbagin and standard drugs were determined via the broth microdilution method to be 0.5 to 8 μg/mL and 0.25–128 μg/mL, respectively. In the resistance modulation study, MICs of the standard drugs were redetermined in the presence of subinhibitory concentration of plumbagin (4 μg/mL), and plumbagin was found to either potentiate or reduce the activities of these standard drugs with the highest potentiation recorded up to 12-folds for ketoconazole against Candida albicans. Plumbagin was found to be bacteriostatic and fungistatic from the time-kill kinetics study. Plumbagin demonstrated strong inhibition of biofilm formation activity at concentrations of 128, 64, and 32 μg/mL against the test microorganisms compared with ciprofloxacin. Plumbagin has been proved through this study to be a suitable lead compound in antimicrobial resistance drug development.http://dx.doi.org/10.1155/2019/1250645 |
| spellingShingle | Emmanuel B. A. Adusei Reimmel K. Adosraku James Oppong-Kyekyeku Cedric D. K. Amengor Yakubu Jibira Resistance Modulation Action, Time-Kill Kinetics Assay, and Inhibition of Biofilm Formation Effects of Plumbagin from Plumbago zeylanica Linn Journal of Tropical Medicine |
| title | Resistance Modulation Action, Time-Kill Kinetics Assay, and Inhibition of Biofilm Formation Effects of Plumbagin from Plumbago zeylanica Linn |
| title_full | Resistance Modulation Action, Time-Kill Kinetics Assay, and Inhibition of Biofilm Formation Effects of Plumbagin from Plumbago zeylanica Linn |
| title_fullStr | Resistance Modulation Action, Time-Kill Kinetics Assay, and Inhibition of Biofilm Formation Effects of Plumbagin from Plumbago zeylanica Linn |
| title_full_unstemmed | Resistance Modulation Action, Time-Kill Kinetics Assay, and Inhibition of Biofilm Formation Effects of Plumbagin from Plumbago zeylanica Linn |
| title_short | Resistance Modulation Action, Time-Kill Kinetics Assay, and Inhibition of Biofilm Formation Effects of Plumbagin from Plumbago zeylanica Linn |
| title_sort | resistance modulation action time kill kinetics assay and inhibition of biofilm formation effects of plumbagin from plumbago zeylanica linn |
| url | http://dx.doi.org/10.1155/2019/1250645 |
| work_keys_str_mv | AT emmanuelbaadusei resistancemodulationactiontimekillkineticsassayandinhibitionofbiofilmformationeffectsofplumbaginfromplumbagozeylanicalinn AT reimmelkadosraku resistancemodulationactiontimekillkineticsassayandinhibitionofbiofilmformationeffectsofplumbaginfromplumbagozeylanicalinn AT jamesoppongkyekyeku resistancemodulationactiontimekillkineticsassayandinhibitionofbiofilmformationeffectsofplumbaginfromplumbagozeylanicalinn AT cedricdkamengor resistancemodulationactiontimekillkineticsassayandinhibitionofbiofilmformationeffectsofplumbaginfromplumbagozeylanicalinn AT yakubujibira resistancemodulationactiontimekillkineticsassayandinhibitionofbiofilmformationeffectsofplumbaginfromplumbagozeylanicalinn |