Antiviral activity of Vigna radiata extract against feline coronavirus in vitro
Feline infectious peritonitis (FIP) is a fatal illness caused by a mutated feline coronavirus (FCoV). This disease is characterized by its complexity, resulting from systemic infection, antibody-dependent enhancement (ADE), and challenges in accessing effective therapeutics. Extract derived from Vig...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Veterinary Quarterly |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/01652176.2024.2349665 |
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| author | Ai-Ai Chou Chung-Hui Lin Yen-Chen Chang Hui-Wen Chang Yi-Chen Lin Chia-Chen Pi Yao-Ming Kan Hao-Fen Chuang Hui-Wen Chen |
| author_facet | Ai-Ai Chou Chung-Hui Lin Yen-Chen Chang Hui-Wen Chang Yi-Chen Lin Chia-Chen Pi Yao-Ming Kan Hao-Fen Chuang Hui-Wen Chen |
| author_sort | Ai-Ai Chou |
| collection | DOAJ |
| description | Feline infectious peritonitis (FIP) is a fatal illness caused by a mutated feline coronavirus (FCoV). This disease is characterized by its complexity, resulting from systemic infection, antibody-dependent enhancement (ADE), and challenges in accessing effective therapeutics. Extract derived from Vigna radiata (L.) R. Wilczek (VRE) exhibits various pharmacological effects, including antiviral activity. This study aimed to investigate the antiviral potential of VRE against FCoV, addressing the urgent need to advance the treatment of FIP. We explored the anti-FCoV activity, antiviral mechanism, and combinational application of VRE by means of in vitro antiviral assays. Our findings reveal that VRE effectively inhibited the cytopathic effect induced by FCoV, reduced viral proliferation, and downregulated spike protein expression. Moreover, VRE blocked FCoV in the early and late infection stages and was effective under in vitro ADE infection. Notably, when combined with VRE, the polymerase inhibitor GS-441524 or protease inhibitor GC376 suppressed FCoV more effectively than monotherapy. In conclusion, this study characterizes the antiviral property of VRE against FCoV in vitro, and VRE possesses therapeutic potential for FCoV treatment. |
| format | Article |
| id | doaj-art-7bbf5bf824654019a28ede2c75660721 |
| institution | OA Journals |
| issn | 0165-2176 1875-5941 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Veterinary Quarterly |
| spelling | doaj-art-7bbf5bf824654019a28ede2c756607212025-08-20T02:19:51ZengTaylor & Francis GroupVeterinary Quarterly0165-21761875-59412024-12-0144111310.1080/01652176.2024.2349665Antiviral activity of Vigna radiata extract against feline coronavirus in vitroAi-Ai Chou0Chung-Hui Lin1Yen-Chen Chang2Hui-Wen Chang3Yi-Chen Lin4Chia-Chen Pi5Yao-Ming Kan6Hao-Fen Chuang7Hui-Wen Chen8Department of Veterinary Medicine, National Taiwan University, Taipei, TaiwanNational Taiwan University Veterinary Hospital, National Taiwan University, Taipei, TaiwanGraduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine, National Taiwan University, Taipei, TaiwanGraduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine, National Taiwan University, Taipei, TaiwanKing’s Ground Biotech Co., Ltd., Pingtung, TaiwanKing’s Ground Biotech Co., Ltd., Pingtung, TaiwanDepartment of Veterinary Medicine, National Taiwan University, Taipei, TaiwanDepartment of Veterinary Medicine, National Taiwan University, Taipei, TaiwanDepartment of Veterinary Medicine, National Taiwan University, Taipei, TaiwanFeline infectious peritonitis (FIP) is a fatal illness caused by a mutated feline coronavirus (FCoV). This disease is characterized by its complexity, resulting from systemic infection, antibody-dependent enhancement (ADE), and challenges in accessing effective therapeutics. Extract derived from Vigna radiata (L.) R. Wilczek (VRE) exhibits various pharmacological effects, including antiviral activity. This study aimed to investigate the antiviral potential of VRE against FCoV, addressing the urgent need to advance the treatment of FIP. We explored the anti-FCoV activity, antiviral mechanism, and combinational application of VRE by means of in vitro antiviral assays. Our findings reveal that VRE effectively inhibited the cytopathic effect induced by FCoV, reduced viral proliferation, and downregulated spike protein expression. Moreover, VRE blocked FCoV in the early and late infection stages and was effective under in vitro ADE infection. Notably, when combined with VRE, the polymerase inhibitor GS-441524 or protease inhibitor GC376 suppressed FCoV more effectively than monotherapy. In conclusion, this study characterizes the antiviral property of VRE against FCoV in vitro, and VRE possesses therapeutic potential for FCoV treatment.https://www.tandfonline.com/doi/10.1080/01652176.2024.2349665Feline coronavirusfeline infectious peritonitisantiviralVigna radiata extractGS-441524GC376 |
| spellingShingle | Ai-Ai Chou Chung-Hui Lin Yen-Chen Chang Hui-Wen Chang Yi-Chen Lin Chia-Chen Pi Yao-Ming Kan Hao-Fen Chuang Hui-Wen Chen Antiviral activity of Vigna radiata extract against feline coronavirus in vitro Veterinary Quarterly Feline coronavirus feline infectious peritonitis antiviral Vigna radiata extract GS-441524 GC376 |
| title | Antiviral activity of Vigna radiata extract against feline coronavirus in vitro |
| title_full | Antiviral activity of Vigna radiata extract against feline coronavirus in vitro |
| title_fullStr | Antiviral activity of Vigna radiata extract against feline coronavirus in vitro |
| title_full_unstemmed | Antiviral activity of Vigna radiata extract against feline coronavirus in vitro |
| title_short | Antiviral activity of Vigna radiata extract against feline coronavirus in vitro |
| title_sort | antiviral activity of vigna radiata extract against feline coronavirus in vitro |
| topic | Feline coronavirus feline infectious peritonitis antiviral Vigna radiata extract GS-441524 GC376 |
| url | https://www.tandfonline.com/doi/10.1080/01652176.2024.2349665 |
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