SADS-CoV nsp5 Inhibits Interferon Production by Targeting Kinase IKKε
Swine acute diarrhea syndrome coronavirus (SADS-CoV), initially identified in China in February 2017, severely impacts the swine industry by causing lethal watery diarrhea in neonatal piglets. Understanding the molecular mechanism employed by SADS-CoV to evade the host’s immune defenses is of utmost...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/13/7/1494 |
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| author | Gaoli She Chunhui Zhong Yue Pan Zexin Chen Jingmin Li Mingchong Li Yufang Liu Yongchang Cao Xiaona Wei Chunyi Xue |
| author_facet | Gaoli She Chunhui Zhong Yue Pan Zexin Chen Jingmin Li Mingchong Li Yufang Liu Yongchang Cao Xiaona Wei Chunyi Xue |
| author_sort | Gaoli She |
| collection | DOAJ |
| description | Swine acute diarrhea syndrome coronavirus (SADS-CoV), initially identified in China in February 2017, severely impacts the swine industry by causing lethal watery diarrhea in neonatal piglets. Understanding the molecular mechanism employed by SADS-CoV to evade the host’s immune defenses is of utmost importance. In this study, using the porcine ileum epithelial cell line IPI-FX as an in vitro model, we investigated the highly pathogenic SADS-CoV GDS04 strain and its nonstructural protein 5 (nsp5) for their roles in inhibiting interferon-beta (IFN-β) production. Our findings indicated that GDS04 inhibited poly(I:C)-induced IFN-β production by impeding the promoter activities of IRF3 and NF-κB. As a 3C-like protease, SADS-CoV nsp5 functioned as an interferon inhibitor by interacting with IKKε, reducing its protein abundance, and inhibiting its phosphorylation. This study enhances our understanding of the interaction between coronaviruses and their hosts, providing novel insights into the evasion of the immune system by coronaviruses. |
| format | Article |
| id | doaj-art-7bbcfbce4b984539a0712bcfddcd57a9 |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-7bbcfbce4b984539a0712bcfddcd57a92025-08-20T03:08:02ZengMDPI AGMicroorganisms2076-26072025-06-01137149410.3390/microorganisms13071494SADS-CoV nsp5 Inhibits Interferon Production by Targeting Kinase IKKεGaoli She0Chunhui Zhong1Yue Pan2Zexin Chen3Jingmin Li4Mingchong Li5Yufang Liu6Yongchang Cao7Xiaona Wei8Chunyi Xue9State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaSwine acute diarrhea syndrome coronavirus (SADS-CoV), initially identified in China in February 2017, severely impacts the swine industry by causing lethal watery diarrhea in neonatal piglets. Understanding the molecular mechanism employed by SADS-CoV to evade the host’s immune defenses is of utmost importance. In this study, using the porcine ileum epithelial cell line IPI-FX as an in vitro model, we investigated the highly pathogenic SADS-CoV GDS04 strain and its nonstructural protein 5 (nsp5) for their roles in inhibiting interferon-beta (IFN-β) production. Our findings indicated that GDS04 inhibited poly(I:C)-induced IFN-β production by impeding the promoter activities of IRF3 and NF-κB. As a 3C-like protease, SADS-CoV nsp5 functioned as an interferon inhibitor by interacting with IKKε, reducing its protein abundance, and inhibiting its phosphorylation. This study enhances our understanding of the interaction between coronaviruses and their hosts, providing novel insights into the evasion of the immune system by coronaviruses.https://www.mdpi.com/2076-2607/13/7/1494SADS-CoVGDS04IFN-βnsp5IKKε |
| spellingShingle | Gaoli She Chunhui Zhong Yue Pan Zexin Chen Jingmin Li Mingchong Li Yufang Liu Yongchang Cao Xiaona Wei Chunyi Xue SADS-CoV nsp5 Inhibits Interferon Production by Targeting Kinase IKKε Microorganisms SADS-CoV GDS04 IFN-β nsp5 IKKε |
| title | SADS-CoV nsp5 Inhibits Interferon Production by Targeting Kinase IKKε |
| title_full | SADS-CoV nsp5 Inhibits Interferon Production by Targeting Kinase IKKε |
| title_fullStr | SADS-CoV nsp5 Inhibits Interferon Production by Targeting Kinase IKKε |
| title_full_unstemmed | SADS-CoV nsp5 Inhibits Interferon Production by Targeting Kinase IKKε |
| title_short | SADS-CoV nsp5 Inhibits Interferon Production by Targeting Kinase IKKε |
| title_sort | sads cov nsp5 inhibits interferon production by targeting kinase ikkε |
| topic | SADS-CoV GDS04 IFN-β nsp5 IKKε |
| url | https://www.mdpi.com/2076-2607/13/7/1494 |
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