Oxidative stress activates the transplanted adipose-derived stem cells to exert antioxidant effects in alopecia treatment
Background: Alopecia is a global dermatological challenge. Adipose-derived stem cells (ADSC) show therapeutic potential, but their mechanisms in promoting hair regrowth, particularly under oxidative stress conditions, remain unclear..Objective: To investigate ADSC's role in promoting hair regro...
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Taylor & Francis Group
2025-12-01
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| Series: | Redox Report |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13510002.2025.2503128 |
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| author | Xuer Sun Minliang Chen |
| author_facet | Xuer Sun Minliang Chen |
| author_sort | Xuer Sun |
| collection | DOAJ |
| description | Background: Alopecia is a global dermatological challenge. Adipose-derived stem cells (ADSC) show therapeutic potential, but their mechanisms in promoting hair regrowth, particularly under oxidative stress conditions, remain unclear..Objective: To investigate ADSC's role in promoting hair regrowth by mitigating oxidative stress.Methods: Using H₂O₂-stressed HaCaT cells, ADSC's protective effects were evaluated via conditioned medium (CM) and co-culture. Assessments included cell viability, colony formation, ROS, MDA, antioxidant enzymes, and 8-OHdG. Nrf2 activation was analyzed by immunofluorescence and Western blot. A mouse radiation injury model validated findings.Results: Non-pretreated ADSC offered limited oxidative protection to HaCaT cells. Conversely, H₂O₂-pretreated ADSC significantly enhanced HaCaT viability and proliferation in both CM and co-culture systems. This involved paracrine activation of the Nrf2 pathway in HaCaT cells, boosting antioxidant enzymes, accelerating ROS clearance, and reducing lipid peroxidation. These effects were reversible with Nrf2 inhibition. In vivo, CM from H₂O₂-stimulated ADSC promoted hair regrowth in irradiated mice, outperforming CM from non-pretreated ADSC by activating Nrf2 and reducing tissue oxidative damage.Conclusions: Oxidative stress potentiates the protective capacity of ADSC against oxidative via Nrf2-dependent paracrine mechanisms, offering a promising strategy for alopecia treatment. |
| format | Article |
| id | doaj-art-7bbaa2f8804e481b9aca4899ba389a07 |
| institution | OA Journals |
| issn | 1351-0002 1743-2928 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Redox Report |
| spelling | doaj-art-7bbaa2f8804e481b9aca4899ba389a072025-08-20T02:05:32ZengTaylor & Francis GroupRedox Report1351-00021743-29282025-12-0130110.1080/13510002.2025.2503128Oxidative stress activates the transplanted adipose-derived stem cells to exert antioxidant effects in alopecia treatmentXuer Sun0Minliang Chen1Senior Department of Burns and Plastic Surgery, The Fourth Medical Center of PLA General Hospital, Beijing, People’s Republic of ChinaSenior Department of Burns and Plastic Surgery, The Fourth Medical Center of PLA General Hospital, Beijing, People’s Republic of ChinaBackground: Alopecia is a global dermatological challenge. Adipose-derived stem cells (ADSC) show therapeutic potential, but their mechanisms in promoting hair regrowth, particularly under oxidative stress conditions, remain unclear..Objective: To investigate ADSC's role in promoting hair regrowth by mitigating oxidative stress.Methods: Using H₂O₂-stressed HaCaT cells, ADSC's protective effects were evaluated via conditioned medium (CM) and co-culture. Assessments included cell viability, colony formation, ROS, MDA, antioxidant enzymes, and 8-OHdG. Nrf2 activation was analyzed by immunofluorescence and Western blot. A mouse radiation injury model validated findings.Results: Non-pretreated ADSC offered limited oxidative protection to HaCaT cells. Conversely, H₂O₂-pretreated ADSC significantly enhanced HaCaT viability and proliferation in both CM and co-culture systems. This involved paracrine activation of the Nrf2 pathway in HaCaT cells, boosting antioxidant enzymes, accelerating ROS clearance, and reducing lipid peroxidation. These effects were reversible with Nrf2 inhibition. In vivo, CM from H₂O₂-stimulated ADSC promoted hair regrowth in irradiated mice, outperforming CM from non-pretreated ADSC by activating Nrf2 and reducing tissue oxidative damage.Conclusions: Oxidative stress potentiates the protective capacity of ADSC against oxidative via Nrf2-dependent paracrine mechanisms, offering a promising strategy for alopecia treatment.https://www.tandfonline.com/doi/10.1080/13510002.2025.2503128Oxidative stressmesenchymal stem cellnanofatalopeciaemulsified fatfat transplantation |
| spellingShingle | Xuer Sun Minliang Chen Oxidative stress activates the transplanted adipose-derived stem cells to exert antioxidant effects in alopecia treatment Redox Report Oxidative stress mesenchymal stem cell nanofat alopecia emulsified fat fat transplantation |
| title | Oxidative stress activates the transplanted adipose-derived stem cells to exert antioxidant effects in alopecia treatment |
| title_full | Oxidative stress activates the transplanted adipose-derived stem cells to exert antioxidant effects in alopecia treatment |
| title_fullStr | Oxidative stress activates the transplanted adipose-derived stem cells to exert antioxidant effects in alopecia treatment |
| title_full_unstemmed | Oxidative stress activates the transplanted adipose-derived stem cells to exert antioxidant effects in alopecia treatment |
| title_short | Oxidative stress activates the transplanted adipose-derived stem cells to exert antioxidant effects in alopecia treatment |
| title_sort | oxidative stress activates the transplanted adipose derived stem cells to exert antioxidant effects in alopecia treatment |
| topic | Oxidative stress mesenchymal stem cell nanofat alopecia emulsified fat fat transplantation |
| url | https://www.tandfonline.com/doi/10.1080/13510002.2025.2503128 |
| work_keys_str_mv | AT xuersun oxidativestressactivatesthetransplantedadiposederivedstemcellstoexertantioxidanteffectsinalopeciatreatment AT minliangchen oxidativestressactivatesthetransplantedadiposederivedstemcellstoexertantioxidanteffectsinalopeciatreatment |