Effect of Vitamin D during Orthodontic Tooth Movement on Receptor Activator of Nuclear Factor Kappa-Â Ligand Expression and Osteoclast Number in Pregnant Wistar Rat (Rattus novergicus)

Background: Female patients have the possibility to become pregnant during orthodontic treatment. Vitamin D usually consumed by pregnant women. Estrogen and Vitamin D could affect bone metabolism. Aim and Objectives: The aim of this study was to analyze the effect of vitamin D during orthodontic mov...

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Main Authors: Ida Bagus Narmada, Kinanthi Rizka Dewi Husodo, I Gusti Aju Wahju Ardani, Dwi Rahmawati, Alexander Patera Nugraha, Regina Purnama Dewi Iskandar
Format: Article
Language:English
Published: Krishna Vishwa Vidyapeeth (Deemed to be University), Karad 2019-01-01
Series:Journal of Krishna Institute of Medical Sciences University
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Online Access:http://www.jkimsu.com/jkimsu-vol8no1/JKIMSU,%20Vol.%208,%20No.%201,%20January-March%202019%20Page%2037-42.pdf
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Summary:Background: Female patients have the possibility to become pregnant during orthodontic treatment. Vitamin D usually consumed by pregnant women. Estrogen and Vitamin D could affect bone metabolism. Aim and Objectives: The aim of this study was to analyze the effect of vitamin D during orthodontic movement in pregnant rats by Receptor Activator of Nuclear Factor Kappa-Â Ligand (RANKL) expression and osteoclast number. Material and Methods: The experimental observational analytic study with post-test only control group design and simple random sampling method was conducted. 24-healthy-female Wistar rats were divided into 4 groups; K1: pregnant rats with orthodontic tooth movement and vitamin D on Day 7; K2: pregnant rats with orthodontic tooth movement and vitamin D on Day 14; K3: pregnant rats with orthodontic tooth movement without vitamin D on Day 7 and; K4: pregnant rats with orthodontic tooth movement without vitamin D on Day 14. Nickle-Titanium coil spring with 10 g/mm2 force was placed between the incisors and the maxillary molars. The RANKL expression and osteoclasts number were analyzed using Analysis of Variance (ANOVA) (p<0.05). Results: The highest osteoclasts number (8.494 ± 1.194), and RANKLexpression (7.967 ± 2.185) found in K1 group with significant between groups (p<0.05).Conclusions: Vitamin D increase osteoclast number and RANKL expression during orthodontic tooth movement in pregnant rats.
ISSN:2231-4261
2231-4261