Transcriptomic comparison analysis across seven developmental stages of the Triatoma rubrofasciata, a vector of Chagas disease
Abstract Background Triatoma rubrofasciata is an obligate hematophagous insect and a primary vector of Trypanosoma cruzi, the etiological agent of Chagas disease, with a widespread global distribution. In addition to Try. cruzi, T. rubrofasciata also serves as a vector for various other pathogens, i...
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2025-05-01
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| Online Access: | https://doi.org/10.1186/s12864-025-11632-8 |
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| author | Lei Duan Yunjia Tian Ziyi Wang Limin Yang Yunhai Guo Yuanyuan Li Zhengbin Zhou Yong Shen Yi Zhang Qin Liu |
| author_facet | Lei Duan Yunjia Tian Ziyi Wang Limin Yang Yunhai Guo Yuanyuan Li Zhengbin Zhou Yong Shen Yi Zhang Qin Liu |
| author_sort | Lei Duan |
| collection | DOAJ |
| description | Abstract Background Triatoma rubrofasciata is an obligate hematophagous insect and a primary vector of Trypanosoma cruzi, the etiological agent of Chagas disease, with a widespread global distribution. In addition to Try. cruzi, T. rubrofasciata also serves as a vector for various other pathogens, including Try. lewisi, Try. conorhini, and Bartonella species. Despite its increasing epidemiological relevance in the transmission of multiple diseases, research on T. rubrofasciata remains limited. Results Differentially expressed genes (DEGs) were associated with growth, development, carbohydrate metabolism, and immunity. Notably, homeobox protein genes, including homeobox protein Nkx-6.2-like, homeobox protein abdominal-B isoform X1, homeobox protein Hox-A3-like, and Hox-B4-like, along with E3 ubiquitin protein ligase genes and sexual differentiation-related genes, such as male-specific lethal 1-like 1 isoform X3 (MSL), transformer-2 protein homolog beta-like isoform X2 (tra-2), and doublesex- and mab-3-related transcription factor A2-like (dsx), were highly expressed in the egg stage. Additionally, venom-related genes, including venom histidine phosphatase-like protein 1 and venom serine carboxypeptidase-like, were predominantly expressed in nymphal stages 4 and 5, while cytochrome P450 CYP425A1v2 exhibited high expression levels in the adult stages. Among these DEGs, we propose that homeobox protein genes, dsx, tra-2, and others may serve as candidate genes involved in growth, development, and sexual differentiation. This study provides valuable insights into gene expression dynamics during T. rubrofasciata development and establishes a foundation for future functional research on this species. Conclusions In this study, we sequenced the complete developmental stages of T. rubrofasciata using HiSeq technology. Our findings offer novel insights into the molecular mechanisms underlying development and sex regulation in this species. Furthermore, the identified differentially expressed genes (DEGs) may serve as potential targets for innovative pest control strategies. |
| format | Article |
| id | doaj-art-7ba0081202864ddbb968f7bf09d74bad |
| institution | OA Journals |
| issn | 1471-2164 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
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| series | BMC Genomics |
| spelling | doaj-art-7ba0081202864ddbb968f7bf09d74bad2025-08-20T01:49:33ZengBMCBMC Genomics1471-21642025-05-0126111610.1186/s12864-025-11632-8Transcriptomic comparison analysis across seven developmental stages of the Triatoma rubrofasciata, a vector of Chagas diseaseLei Duan0Yunjia Tian1Ziyi Wang2Limin Yang3Yunhai Guo4Yuanyuan Li5Zhengbin Zhou6Yong Shen7Yi Zhang8Qin Liu9NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases at Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), National Center for International Research on Tropical DiseasesNHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases at Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), National Center for International Research on Tropical DiseasesNHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases at Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), National Center for International Research on Tropical DiseasesNHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases at Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), National Center for International Research on Tropical DiseasesNHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases at Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), National Center for International Research on Tropical DiseasesNHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases at Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), National Center for International Research on Tropical DiseasesNHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases at Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), National Center for International Research on Tropical DiseasesNHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases at Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), National Center for International Research on Tropical DiseasesNHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases at Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), National Center for International Research on Tropical DiseasesNHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases at Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), National Center for International Research on Tropical DiseasesAbstract Background Triatoma rubrofasciata is an obligate hematophagous insect and a primary vector of Trypanosoma cruzi, the etiological agent of Chagas disease, with a widespread global distribution. In addition to Try. cruzi, T. rubrofasciata also serves as a vector for various other pathogens, including Try. lewisi, Try. conorhini, and Bartonella species. Despite its increasing epidemiological relevance in the transmission of multiple diseases, research on T. rubrofasciata remains limited. Results Differentially expressed genes (DEGs) were associated with growth, development, carbohydrate metabolism, and immunity. Notably, homeobox protein genes, including homeobox protein Nkx-6.2-like, homeobox protein abdominal-B isoform X1, homeobox protein Hox-A3-like, and Hox-B4-like, along with E3 ubiquitin protein ligase genes and sexual differentiation-related genes, such as male-specific lethal 1-like 1 isoform X3 (MSL), transformer-2 protein homolog beta-like isoform X2 (tra-2), and doublesex- and mab-3-related transcription factor A2-like (dsx), were highly expressed in the egg stage. Additionally, venom-related genes, including venom histidine phosphatase-like protein 1 and venom serine carboxypeptidase-like, were predominantly expressed in nymphal stages 4 and 5, while cytochrome P450 CYP425A1v2 exhibited high expression levels in the adult stages. Among these DEGs, we propose that homeobox protein genes, dsx, tra-2, and others may serve as candidate genes involved in growth, development, and sexual differentiation. This study provides valuable insights into gene expression dynamics during T. rubrofasciata development and establishes a foundation for future functional research on this species. Conclusions In this study, we sequenced the complete developmental stages of T. rubrofasciata using HiSeq technology. Our findings offer novel insights into the molecular mechanisms underlying development and sex regulation in this species. Furthermore, the identified differentially expressed genes (DEGs) may serve as potential targets for innovative pest control strategies.https://doi.org/10.1186/s12864-025-11632-8Neglected tropical diseasesTriatominesT. rubrofasciataDifferential gene expression analysis |
| spellingShingle | Lei Duan Yunjia Tian Ziyi Wang Limin Yang Yunhai Guo Yuanyuan Li Zhengbin Zhou Yong Shen Yi Zhang Qin Liu Transcriptomic comparison analysis across seven developmental stages of the Triatoma rubrofasciata, a vector of Chagas disease BMC Genomics Neglected tropical diseases Triatomines T. rubrofasciata Differential gene expression analysis |
| title | Transcriptomic comparison analysis across seven developmental stages of the Triatoma rubrofasciata, a vector of Chagas disease |
| title_full | Transcriptomic comparison analysis across seven developmental stages of the Triatoma rubrofasciata, a vector of Chagas disease |
| title_fullStr | Transcriptomic comparison analysis across seven developmental stages of the Triatoma rubrofasciata, a vector of Chagas disease |
| title_full_unstemmed | Transcriptomic comparison analysis across seven developmental stages of the Triatoma rubrofasciata, a vector of Chagas disease |
| title_short | Transcriptomic comparison analysis across seven developmental stages of the Triatoma rubrofasciata, a vector of Chagas disease |
| title_sort | transcriptomic comparison analysis across seven developmental stages of the triatoma rubrofasciata a vector of chagas disease |
| topic | Neglected tropical diseases Triatomines T. rubrofasciata Differential gene expression analysis |
| url | https://doi.org/10.1186/s12864-025-11632-8 |
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