Evaluation of In Vitro Antimalarial Activity of Different Extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and Fractions of the Most Potent Extracts
Ten extracts with different polarity from two Iranian Artemisia species, A. armeniaca Lam. and A. aucheri Boiss, were screened for their antimalarial properties by in vitro β-hematin formation assay. Dichloromethane (DCM) extracts of both plants showed significant antimalarial activities with IC50...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/825370 |
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| author | Mahdi Mojarrab Ali Shiravand Abbas Delazar Fariba Heshmati Afshar |
| author_facet | Mahdi Mojarrab Ali Shiravand Abbas Delazar Fariba Heshmati Afshar |
| author_sort | Mahdi Mojarrab |
| collection | DOAJ |
| description | Ten extracts with different polarity from two Iranian Artemisia species, A. armeniaca Lam. and A. aucheri Boiss, were screened for their antimalarial properties by in vitro β-hematin formation assay. Dichloromethane (DCM) extracts of both plants showed significant antimalarial activities with IC50 values of 1.36 ± 0.01 and 1.83 ± 0.03 mg/mL and IC90 values of 2.12 ± 0.04 and 2.62 ± 0.09 mg/mL for A. armeniaca and A. aucheri, respectively. Bioactivity-guided fractionation of DCM extracts of both plants by vacuum liquid chromatography (VLC) over silica gel with solvent mixtures of increasing polarities afforded seven fractions. Two fractions from DCM extract of A. armeniaca and four fractions from DCM extract of A. aucheri showed potent antimalarial activity with reducing IC50 and IC90 values compared to extracts. The most potent fraction belonged to DCM extract of A. armeniaca with IC50 and IC90 values of 0.47 ± 0.006 and 0.71 ± 0.006 mg/mL, respectively. |
| format | Article |
| id | doaj-art-7ba002d629dd48c2b278002ea03e80bd |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-7ba002d629dd48c2b278002ea03e80bd2025-08-20T03:35:43ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/825370825370Evaluation of In Vitro Antimalarial Activity of Different Extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and Fractions of the Most Potent ExtractsMahdi Mojarrab0Ali Shiravand1Abbas Delazar2Fariba Heshmati Afshar3Novel Drug Delivery Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah 67346-67149, IranNovel Drug Delivery Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah 67346-67149, IranDrug Applied Research Centre, Tabriz University of Medical Sciences, Tabriz 51656-65811, IranMedical Philosophy and History Research Center, Tabriz University of Medical Sciences, Tabriz 371-51665, IranTen extracts with different polarity from two Iranian Artemisia species, A. armeniaca Lam. and A. aucheri Boiss, were screened for their antimalarial properties by in vitro β-hematin formation assay. Dichloromethane (DCM) extracts of both plants showed significant antimalarial activities with IC50 values of 1.36 ± 0.01 and 1.83 ± 0.03 mg/mL and IC90 values of 2.12 ± 0.04 and 2.62 ± 0.09 mg/mL for A. armeniaca and A. aucheri, respectively. Bioactivity-guided fractionation of DCM extracts of both plants by vacuum liquid chromatography (VLC) over silica gel with solvent mixtures of increasing polarities afforded seven fractions. Two fractions from DCM extract of A. armeniaca and four fractions from DCM extract of A. aucheri showed potent antimalarial activity with reducing IC50 and IC90 values compared to extracts. The most potent fraction belonged to DCM extract of A. armeniaca with IC50 and IC90 values of 0.47 ± 0.006 and 0.71 ± 0.006 mg/mL, respectively.http://dx.doi.org/10.1155/2014/825370 |
| spellingShingle | Mahdi Mojarrab Ali Shiravand Abbas Delazar Fariba Heshmati Afshar Evaluation of In Vitro Antimalarial Activity of Different Extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and Fractions of the Most Potent Extracts The Scientific World Journal |
| title | Evaluation of In Vitro Antimalarial Activity of Different Extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and Fractions of the Most Potent Extracts |
| title_full | Evaluation of In Vitro Antimalarial Activity of Different Extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and Fractions of the Most Potent Extracts |
| title_fullStr | Evaluation of In Vitro Antimalarial Activity of Different Extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and Fractions of the Most Potent Extracts |
| title_full_unstemmed | Evaluation of In Vitro Antimalarial Activity of Different Extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and Fractions of the Most Potent Extracts |
| title_short | Evaluation of In Vitro Antimalarial Activity of Different Extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and Fractions of the Most Potent Extracts |
| title_sort | evaluation of in vitro antimalarial activity of different extracts of artemisia aucheri boiss and a armeniaca lam and fractions of the most potent extracts |
| url | http://dx.doi.org/10.1155/2014/825370 |
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