The Antibacterial Effect In Vitro of Honey Derived from Various Danish Flora
The mechanism behind the biologic actions of honey as a wound remedy has been intensively studied; however, there is no published data regarding any antibacterial effect of honey derived from Danish flora. We surveyed 11 honeys of various Danish floral sources for their antibacterial activity and co...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Dermatology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2018/7021713 |
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| author | Reem Dina Matzen Julie Zinck Leth-Espensen Therese Jansson Dennis Sandris Nielsen Marianne N. Lund Steen Matzen |
| author_facet | Reem Dina Matzen Julie Zinck Leth-Espensen Therese Jansson Dennis Sandris Nielsen Marianne N. Lund Steen Matzen |
| author_sort | Reem Dina Matzen |
| collection | DOAJ |
| description | The mechanism behind the biologic actions of honey as a wound remedy has been intensively studied; however, there is no published data regarding any antibacterial effect of honey derived from Danish flora. We surveyed 11 honeys of various Danish floral sources for their antibacterial activity and compared them to a culinary processed commercial honey (Jakobsens) and a raw and a medical grade Manuka (Leptospermum scoparium) honey using the agar-well diffusion method. We tested the effect on three gram-positive bacteria (two strains of Staphylococcus aureus and one strain of Staphylococcus epidermidis) and two gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli). All samples, except the commercial honey, exhibited antibacterial activity, and samples derived from Water Mint (Mentha aquatica), Organic 2 (mixed organic flora), and Linden (Tilia cordata) honey had consistent effects on all bacteria tested and showed greater effect than medical grade and raw Manuka (L. scoparium) honey. The content of methylglyoxal was low in the Danish honey (< 2 μg/mL) and significantly (p<0.05) higher in both the raw and the medical grade Manuka (L. scoparium) honey, where the concentrations were, respectively, 6.29 μg/mL and 54.33 μg/mL. The antibacterial effect of Danish honeys was mostly due to hydrogen peroxide. We conclude that honeys derived from Danish flora possess antibacterial effect, probably by a hurdle effect of viscosity, osmolality, acidity, bioactive peptides, and most importantly the content of hydrogen peroxide. These findings indicate that honeys of various Danish floral sources may have clinical potential, although further studies are necessary to elucidate this in order to determine whether the results of our in vitro experiments also apply to a clinical setting. |
| format | Article |
| id | doaj-art-7b9bd54df07f44f8be32d9128ebd45b9 |
| institution | Kabale University |
| issn | 1687-6105 1687-6113 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Dermatology Research and Practice |
| spelling | doaj-art-7b9bd54df07f44f8be32d9128ebd45b92025-02-03T06:13:11ZengWileyDermatology Research and Practice1687-61051687-61132018-01-01201810.1155/2018/70217137021713The Antibacterial Effect In Vitro of Honey Derived from Various Danish FloraReem Dina Matzen0Julie Zinck Leth-Espensen1Therese Jansson2Dennis Sandris Nielsen3Marianne N. Lund4Steen Matzen5Department of Plastic Surgery and Breast Surgery, Zealand University Hospital, Roskilde, DenmarkDepartment of Food Science, Faculty of Science, University of Copenhagen, DenmarkDepartment of Food Science, Faculty of Science, University of Copenhagen, DenmarkDepartment of Food Science, Faculty of Science, University of Copenhagen, DenmarkDepartment of Food Science, Faculty of Science, University of Copenhagen, DenmarkDepartment of Plastic Surgery and Breast Surgery, Zealand University Hospital, Roskilde, DenmarkThe mechanism behind the biologic actions of honey as a wound remedy has been intensively studied; however, there is no published data regarding any antibacterial effect of honey derived from Danish flora. We surveyed 11 honeys of various Danish floral sources for their antibacterial activity and compared them to a culinary processed commercial honey (Jakobsens) and a raw and a medical grade Manuka (Leptospermum scoparium) honey using the agar-well diffusion method. We tested the effect on three gram-positive bacteria (two strains of Staphylococcus aureus and one strain of Staphylococcus epidermidis) and two gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli). All samples, except the commercial honey, exhibited antibacterial activity, and samples derived from Water Mint (Mentha aquatica), Organic 2 (mixed organic flora), and Linden (Tilia cordata) honey had consistent effects on all bacteria tested and showed greater effect than medical grade and raw Manuka (L. scoparium) honey. The content of methylglyoxal was low in the Danish honey (< 2 μg/mL) and significantly (p<0.05) higher in both the raw and the medical grade Manuka (L. scoparium) honey, where the concentrations were, respectively, 6.29 μg/mL and 54.33 μg/mL. The antibacterial effect of Danish honeys was mostly due to hydrogen peroxide. We conclude that honeys derived from Danish flora possess antibacterial effect, probably by a hurdle effect of viscosity, osmolality, acidity, bioactive peptides, and most importantly the content of hydrogen peroxide. These findings indicate that honeys of various Danish floral sources may have clinical potential, although further studies are necessary to elucidate this in order to determine whether the results of our in vitro experiments also apply to a clinical setting.http://dx.doi.org/10.1155/2018/7021713 |
| spellingShingle | Reem Dina Matzen Julie Zinck Leth-Espensen Therese Jansson Dennis Sandris Nielsen Marianne N. Lund Steen Matzen The Antibacterial Effect In Vitro of Honey Derived from Various Danish Flora Dermatology Research and Practice |
| title | The Antibacterial Effect In Vitro of Honey Derived from Various Danish Flora |
| title_full | The Antibacterial Effect In Vitro of Honey Derived from Various Danish Flora |
| title_fullStr | The Antibacterial Effect In Vitro of Honey Derived from Various Danish Flora |
| title_full_unstemmed | The Antibacterial Effect In Vitro of Honey Derived from Various Danish Flora |
| title_short | The Antibacterial Effect In Vitro of Honey Derived from Various Danish Flora |
| title_sort | antibacterial effect in vitro of honey derived from various danish flora |
| url | http://dx.doi.org/10.1155/2018/7021713 |
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