Caprine Endometrial Mesenchymal Stromal Stem Cell: Multilineage Potential, Characterization, and Growth Kinetics in Breeding and Anestrous Stages
The endometrial layer of the uterus contains a population of cells with similar characteristics of mesenchymal stem cells (MSCs). In the present study, caprine endometrial mesenchymal stromal stem cells (En-MSCs) characters and differentiation potential to chondrogenic, osteogenic, and adipogenic ce...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2017/5052801 |
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| author | Amin Tamadon Davood Mehrabani Younes Zarezadeh Farhad Rahmanifar Mehdi Dianatpour Shahrokh Zare |
| author_facet | Amin Tamadon Davood Mehrabani Younes Zarezadeh Farhad Rahmanifar Mehdi Dianatpour Shahrokh Zare |
| author_sort | Amin Tamadon |
| collection | DOAJ |
| description | The endometrial layer of the uterus contains a population of cells with similar characteristics of mesenchymal stem cells (MSCs). In the present study, caprine endometrial mesenchymal stromal stem cells (En-MSCs) characters and differentiation potential to chondrogenic, osteogenic, and adipogenic cell lines as well as their growth kinetics in breeding and anestrous stages were evaluated. En-MSCs were enzymatically isolated from endometrial layer of the uterus of adult goats and were cultured and subcultured until passage 4. The growth kinetics and population doubling time (PDT) of caprine En-MSCs in breeding and anestrous stages were determined. En-MSCs in passage 4 were used for the karyotyping and differentiation into chondrocytes, osteocytes, and adipocytes. The PDT in anestrus phase was 40.6 h and in cyclic goats was 53 h. En-MSCs were fibroblast-like in all passages. The number of chromosomes was normal (2n=60) with no chromosomal instability. Chondrogenic, osteogenic, and adipogenic differentiation of En-MSCs was confirmed by staining with Alcian blue, Alizarin red, and Oil Red O, respectively. Caprine En-MSCs demonstrated to be an alternative source of MSCs for cell therapy purposes in regenerative medicine. |
| format | Article |
| id | doaj-art-7b97630e7f1b488e9b9cfb0cc80fdcb4 |
| institution | OA Journals |
| issn | 2090-8113 2042-0048 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-7b97630e7f1b488e9b9cfb0cc80fdcb42025-08-20T02:06:54ZengWileyVeterinary Medicine International2090-81132042-00482017-01-01201710.1155/2017/50528015052801Caprine Endometrial Mesenchymal Stromal Stem Cell: Multilineage Potential, Characterization, and Growth Kinetics in Breeding and Anestrous StagesAmin Tamadon0Davood Mehrabani1Younes Zarezadeh2Farhad Rahmanifar3Mehdi Dianatpour4Shahrokh Zare5Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IranStem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IranSchool of Veterinary Medicine, Shiraz University, Shiraz, IranDepartment of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, IranStem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IranStem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IranThe endometrial layer of the uterus contains a population of cells with similar characteristics of mesenchymal stem cells (MSCs). In the present study, caprine endometrial mesenchymal stromal stem cells (En-MSCs) characters and differentiation potential to chondrogenic, osteogenic, and adipogenic cell lines as well as their growth kinetics in breeding and anestrous stages were evaluated. En-MSCs were enzymatically isolated from endometrial layer of the uterus of adult goats and were cultured and subcultured until passage 4. The growth kinetics and population doubling time (PDT) of caprine En-MSCs in breeding and anestrous stages were determined. En-MSCs in passage 4 were used for the karyotyping and differentiation into chondrocytes, osteocytes, and adipocytes. The PDT in anestrus phase was 40.6 h and in cyclic goats was 53 h. En-MSCs were fibroblast-like in all passages. The number of chromosomes was normal (2n=60) with no chromosomal instability. Chondrogenic, osteogenic, and adipogenic differentiation of En-MSCs was confirmed by staining with Alcian blue, Alizarin red, and Oil Red O, respectively. Caprine En-MSCs demonstrated to be an alternative source of MSCs for cell therapy purposes in regenerative medicine.http://dx.doi.org/10.1155/2017/5052801 |
| spellingShingle | Amin Tamadon Davood Mehrabani Younes Zarezadeh Farhad Rahmanifar Mehdi Dianatpour Shahrokh Zare Caprine Endometrial Mesenchymal Stromal Stem Cell: Multilineage Potential, Characterization, and Growth Kinetics in Breeding and Anestrous Stages Veterinary Medicine International |
| title | Caprine Endometrial Mesenchymal Stromal Stem Cell: Multilineage Potential, Characterization, and Growth Kinetics in Breeding and Anestrous Stages |
| title_full | Caprine Endometrial Mesenchymal Stromal Stem Cell: Multilineage Potential, Characterization, and Growth Kinetics in Breeding and Anestrous Stages |
| title_fullStr | Caprine Endometrial Mesenchymal Stromal Stem Cell: Multilineage Potential, Characterization, and Growth Kinetics in Breeding and Anestrous Stages |
| title_full_unstemmed | Caprine Endometrial Mesenchymal Stromal Stem Cell: Multilineage Potential, Characterization, and Growth Kinetics in Breeding and Anestrous Stages |
| title_short | Caprine Endometrial Mesenchymal Stromal Stem Cell: Multilineage Potential, Characterization, and Growth Kinetics in Breeding and Anestrous Stages |
| title_sort | caprine endometrial mesenchymal stromal stem cell multilineage potential characterization and growth kinetics in breeding and anestrous stages |
| url | http://dx.doi.org/10.1155/2017/5052801 |
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