Prussian blue nanofilm-sensitized plasmonic electrochemical microscopy for spatially resolved detection of the localized delivery of hydrogen peroxide
Hydrogen peroxide (H2O2) sensing has been widely investigated using various electrochemical methods, yet the challenge of finding an imaging technique capable of real-time, spatially resolved detection remains. Addressing this, we introduce a Prussian blue (PB) nanofilm-sensitized plasmonic electroc...
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| Language: | English |
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Elsevier
2024-12-01
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| Series: | Sensors and Actuators Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666053924000341 |
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| author | Adaly Garcia Christina Dhoj Samuel Groysman Kinsley Wang Stellina Ao Aimee Anguiano Tony Tran Dianlu Jiang Yixian Wang |
| author_facet | Adaly Garcia Christina Dhoj Samuel Groysman Kinsley Wang Stellina Ao Aimee Anguiano Tony Tran Dianlu Jiang Yixian Wang |
| author_sort | Adaly Garcia |
| collection | DOAJ |
| description | Hydrogen peroxide (H2O2) sensing has been widely investigated using various electrochemical methods, yet the challenge of finding an imaging technique capable of real-time, spatially resolved detection remains. Addressing this, we introduce a Prussian blue (PB) nanofilm-sensitized plasmonic electrochemical microscopy (PEM) technique that successfully visualizes the localized delivery of H2O2. The PB nanofilm was carefully characterized, and its sensing capability towards H2O2 was demonstrated in amperometric mode. Employing a precise micromanipulator system, we controlled a micropipette to create a localized concentration gradient on the sensor surface and monitored the gradient through the PB nanofilm-sensitized PEM. The accuracy of the obtained concentration values was further validated by numerical simulations based on finite-element methods. Our technique ensures dependable localized detection, and we anticipate that advancements in film uniformity will further improve the resolution. The potential applications of this technique are broad and significant, including the opportunity to investigate single-cell exocytosis with neurotransmitters like dopamine, thus offering a promising avenue for future biomedical research. |
| format | Article |
| id | doaj-art-7b9181f0049a456ea6e7f67198143b2e |
| institution | Kabale University |
| issn | 2666-0539 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensors and Actuators Reports |
| spelling | doaj-art-7b9181f0049a456ea6e7f67198143b2e2024-11-15T06:14:58ZengElsevierSensors and Actuators Reports2666-05392024-12-018100218Prussian blue nanofilm-sensitized plasmonic electrochemical microscopy for spatially resolved detection of the localized delivery of hydrogen peroxideAdaly Garcia0Christina Dhoj1Samuel Groysman2Kinsley Wang3Stellina Ao4Aimee Anguiano5Tony Tran6Dianlu Jiang7Yixian Wang8Department of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, USADepartment of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, USADepartment of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, USADepartment of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, USADepartment of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, USADepartment of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, USADepartment of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, USADepartment of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, USACorresponding author at: Department of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, USA.; Department of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, USAHydrogen peroxide (H2O2) sensing has been widely investigated using various electrochemical methods, yet the challenge of finding an imaging technique capable of real-time, spatially resolved detection remains. Addressing this, we introduce a Prussian blue (PB) nanofilm-sensitized plasmonic electrochemical microscopy (PEM) technique that successfully visualizes the localized delivery of H2O2. The PB nanofilm was carefully characterized, and its sensing capability towards H2O2 was demonstrated in amperometric mode. Employing a precise micromanipulator system, we controlled a micropipette to create a localized concentration gradient on the sensor surface and monitored the gradient through the PB nanofilm-sensitized PEM. The accuracy of the obtained concentration values was further validated by numerical simulations based on finite-element methods. Our technique ensures dependable localized detection, and we anticipate that advancements in film uniformity will further improve the resolution. The potential applications of this technique are broad and significant, including the opportunity to investigate single-cell exocytosis with neurotransmitters like dopamine, thus offering a promising avenue for future biomedical research.http://www.sciencedirect.com/science/article/pii/S2666053924000341Prussian blueElectrochemistryHydrogen peroxide sensingSurface plasmon resonanceDye-sensitized imagingNumerical simulations |
| spellingShingle | Adaly Garcia Christina Dhoj Samuel Groysman Kinsley Wang Stellina Ao Aimee Anguiano Tony Tran Dianlu Jiang Yixian Wang Prussian blue nanofilm-sensitized plasmonic electrochemical microscopy for spatially resolved detection of the localized delivery of hydrogen peroxide Sensors and Actuators Reports Prussian blue Electrochemistry Hydrogen peroxide sensing Surface plasmon resonance Dye-sensitized imaging Numerical simulations |
| title | Prussian blue nanofilm-sensitized plasmonic electrochemical microscopy for spatially resolved detection of the localized delivery of hydrogen peroxide |
| title_full | Prussian blue nanofilm-sensitized plasmonic electrochemical microscopy for spatially resolved detection of the localized delivery of hydrogen peroxide |
| title_fullStr | Prussian blue nanofilm-sensitized plasmonic electrochemical microscopy for spatially resolved detection of the localized delivery of hydrogen peroxide |
| title_full_unstemmed | Prussian blue nanofilm-sensitized plasmonic electrochemical microscopy for spatially resolved detection of the localized delivery of hydrogen peroxide |
| title_short | Prussian blue nanofilm-sensitized plasmonic electrochemical microscopy for spatially resolved detection of the localized delivery of hydrogen peroxide |
| title_sort | prussian blue nanofilm sensitized plasmonic electrochemical microscopy for spatially resolved detection of the localized delivery of hydrogen peroxide |
| topic | Prussian blue Electrochemistry Hydrogen peroxide sensing Surface plasmon resonance Dye-sensitized imaging Numerical simulations |
| url | http://www.sciencedirect.com/science/article/pii/S2666053924000341 |
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