Circulating PIWI-interacting RNAs in Acute Ischemic Stroke patients
Background: Stroke refers to an abrupt neurological deficit, caused by an acute focal injury of the central nervous system via infarction or hemorrhage due to impaired vascularity, and remains among the leading causes of disability and death worldwide. Stroke is often preceded by an episode of neuro...
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KeAi Communications Co., Ltd.
2025-04-01
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| Series: | Non-coding RNA Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468054025000174 |
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| author | Salman M. Toor Eman K. Aldous Aijaz Parray Naveed Akhtar Yasser Al-Sarraj Abdelilah Arredouani Ghulam Jeelani Pir Sajitha V. Pananchikkal Omar El-Agnaf Ashfaq Shuaib Nehad M. Alajez Omar M.E. Albagha |
| author_facet | Salman M. Toor Eman K. Aldous Aijaz Parray Naveed Akhtar Yasser Al-Sarraj Abdelilah Arredouani Ghulam Jeelani Pir Sajitha V. Pananchikkal Omar El-Agnaf Ashfaq Shuaib Nehad M. Alajez Omar M.E. Albagha |
| author_sort | Salman M. Toor |
| collection | DOAJ |
| description | Background: Stroke refers to an abrupt neurological deficit, caused by an acute focal injury of the central nervous system via infarction or hemorrhage due to impaired vascularity, and remains among the leading causes of disability and death worldwide. Stroke is often preceded by an episode of neuronal deficit termed transient ischemic attack (TIA), which presents an effective opportunity for mitigating the risk of an eminent acute ischemic stroke (AIS). Circulating non-coding RNAs (ncRNAs) have emerged as important biomarkers for stroke, but PIWI-interacting RNAs (piRNAs), a class of small regulatory ncRNAs, have not been previously explored as diagnostic or prognostic biomarkers for stroke. Methods: We conducted comprehensive circulating piRNA profiling of AIS and TIA patients using RNA-seq on serum samples collected within 24 h of clinical diagnosis. The study cohort was divided into discovery and cross-validation datasets to identify replicated piRNAs using stringent analysis cut-offs. The expression levels of the panel of differentially regulated piRNAs between AIS and TIA patients were also compared with healthy controls. Results: We identified a panel of 10 differentially regulated piRNAs between AIS and TIA patients; hsa-piR-28272, -piR-32972, -piR-28247, -piR-24553, -piR-24552, -piR-28275, -piR-28707 and -piR-32882 were upregulated, while hsa-piR-23058 and -piR-23136 were downregulated in AIS patients. Moreover, these 10 piRNAs were also differentially expressed in AIS patients compared to healthy controls. In addition, we investigated the potential gene targets of the dysregulated piRNAs and their plausible involvement in pathophysiological processes affected in stroke. Conclusions: The imbalances in the circulating piRnome of AIS and TIA patients presented herein provide important insights into the roles of piRNAs following ischemic brain injury and potentially provide opportunities to mitigate stroke-induced mortality and morbidity. |
| format | Article |
| id | doaj-art-7b9037c553f64b19b4e1ff35319a8db8 |
| institution | OA Journals |
| issn | 2468-0540 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Non-coding RNA Research |
| spelling | doaj-art-7b9037c553f64b19b4e1ff35319a8db82025-08-20T02:13:40ZengKeAi Communications Co., Ltd.Non-coding RNA Research2468-05402025-04-011129430210.1016/j.ncrna.2025.01.005Circulating PIWI-interacting RNAs in Acute Ischemic Stroke patientsSalman M. Toor0Eman K. Aldous1Aijaz Parray2Naveed Akhtar3Yasser Al-Sarraj4Abdelilah Arredouani5Ghulam Jeelani Pir6Sajitha V. Pananchikkal7Omar El-Agnaf8Ashfaq Shuaib9Nehad M. Alajez10Omar M.E. Albagha11College of Health and Life Sciences (CHLS), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), P.O. Box 34110, Doha, Qatar; Corresponding authors. College of Health and Life Sciences (CHLS), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), P.O. Box 34110, Doha, Qatar.College of Health and Life Sciences (CHLS), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), P.O. Box 34110, Doha, QatarThe Neuroscience Institute, Academic Health System, Hamad Medical Corporation (HMC), P.O. Box 3050, Doha, QatarThe Neuroscience Institute, Academic Health System, Hamad Medical Corporation (HMC), P.O. Box 3050, Doha, Qatar; Department of Internal Medicine, University of Manitoba, MB R3A 1R9, Winnipeg, CanadaQatar Genome Program (QGP), Qatar Foundation Research, Development and Innovation, Qatar Foundation (QF), P.O. Box 5825, Doha, QatarCollege of Health and Life Sciences (CHLS), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), P.O. Box 34110, Doha, Qatar; Diabetes Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), P.O. Box 34110, Doha, QatarThe Neuroscience Institute, Academic Health System, Hamad Medical Corporation (HMC), P.O. Box 3050, Doha, QatarThe Neuroscience Institute, Academic Health System, Hamad Medical Corporation (HMC), P.O. Box 3050, Doha, QatarNeurological Disorders Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), P.O. Box 34110, Doha, QatarDivision of Neurology, Department of Medicine, University of Alberta, AB T6G 2R3, Edmonton, Canada; Department of Neurology, Hamad Medical Corporation (HMC), P.O. Box 5825, Doha, QatarCollege of Health and Life Sciences (CHLS), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), P.O. Box 34110, Doha, Qatar; Translational Oncology Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), P.O. Box 34110, Doha, QatarCollege of Health and Life Sciences (CHLS), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), P.O. Box 34110, Doha, Qatar; Diabetes Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), P.O. Box 34110, Doha, Qatar; Centre for Genomic and Experimental Medicine, Institute of Genetics and Cancer, University of Edinburgh, EH4 2XU, Edinburgh, UK; Corresponding authors. College of Health and Life Sciences (CHLS), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), P.O. Box 34110, Doha, Qatar.Background: Stroke refers to an abrupt neurological deficit, caused by an acute focal injury of the central nervous system via infarction or hemorrhage due to impaired vascularity, and remains among the leading causes of disability and death worldwide. Stroke is often preceded by an episode of neuronal deficit termed transient ischemic attack (TIA), which presents an effective opportunity for mitigating the risk of an eminent acute ischemic stroke (AIS). Circulating non-coding RNAs (ncRNAs) have emerged as important biomarkers for stroke, but PIWI-interacting RNAs (piRNAs), a class of small regulatory ncRNAs, have not been previously explored as diagnostic or prognostic biomarkers for stroke. Methods: We conducted comprehensive circulating piRNA profiling of AIS and TIA patients using RNA-seq on serum samples collected within 24 h of clinical diagnosis. The study cohort was divided into discovery and cross-validation datasets to identify replicated piRNAs using stringent analysis cut-offs. The expression levels of the panel of differentially regulated piRNAs between AIS and TIA patients were also compared with healthy controls. Results: We identified a panel of 10 differentially regulated piRNAs between AIS and TIA patients; hsa-piR-28272, -piR-32972, -piR-28247, -piR-24553, -piR-24552, -piR-28275, -piR-28707 and -piR-32882 were upregulated, while hsa-piR-23058 and -piR-23136 were downregulated in AIS patients. Moreover, these 10 piRNAs were also differentially expressed in AIS patients compared to healthy controls. In addition, we investigated the potential gene targets of the dysregulated piRNAs and their plausible involvement in pathophysiological processes affected in stroke. Conclusions: The imbalances in the circulating piRnome of AIS and TIA patients presented herein provide important insights into the roles of piRNAs following ischemic brain injury and potentially provide opportunities to mitigate stroke-induced mortality and morbidity.http://www.sciencedirect.com/science/article/pii/S2468054025000174Non-coding RNAPIWIpiRNAIschemicStrokeBiomarkers |
| spellingShingle | Salman M. Toor Eman K. Aldous Aijaz Parray Naveed Akhtar Yasser Al-Sarraj Abdelilah Arredouani Ghulam Jeelani Pir Sajitha V. Pananchikkal Omar El-Agnaf Ashfaq Shuaib Nehad M. Alajez Omar M.E. Albagha Circulating PIWI-interacting RNAs in Acute Ischemic Stroke patients Non-coding RNA Research Non-coding RNA PIWI piRNA Ischemic Stroke Biomarkers |
| title | Circulating PIWI-interacting RNAs in Acute Ischemic Stroke patients |
| title_full | Circulating PIWI-interacting RNAs in Acute Ischemic Stroke patients |
| title_fullStr | Circulating PIWI-interacting RNAs in Acute Ischemic Stroke patients |
| title_full_unstemmed | Circulating PIWI-interacting RNAs in Acute Ischemic Stroke patients |
| title_short | Circulating PIWI-interacting RNAs in Acute Ischemic Stroke patients |
| title_sort | circulating piwi interacting rnas in acute ischemic stroke patients |
| topic | Non-coding RNA PIWI piRNA Ischemic Stroke Biomarkers |
| url | http://www.sciencedirect.com/science/article/pii/S2468054025000174 |
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