Conditioned Medium of IGF1-Induced Human Wharton’s Jelly Mesenchymal Stem Cells Effects on Sox9 Gene Expression and Level of COL2 and IL1β in Osteoarthritic Chondrocytes
Wharton’s jelly mesenchymal stem cells (WJMSCs) hold promises for treating osteoarthritis (OA) due to their great capacity for regeneration and their accessibility. However, obstacles like poor cell survival and differentiation prevent them from being used in transplants. Thus, this study examined...
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ITB Journal Publisher
2024-12-01
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| Series: | Journal of Mathematical and Fundamental Sciences |
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| Online Access: | http://167.205.195.146/ojsnew/index.php/jmfs/article/view/18326 |
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| author | Wahyu Widowati Hanna Sari Widya Kusuma Rizal Azis The Fransiska Eltania Faradhina Salfa Nindya |
| author_facet | Wahyu Widowati Hanna Sari Widya Kusuma Rizal Azis The Fransiska Eltania Faradhina Salfa Nindya |
| author_sort | Wahyu Widowati |
| collection | DOAJ |
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Wharton’s jelly mesenchymal stem cells (WJMSCs) hold promises for treating osteoarthritis (OA) due to their great capacity for regeneration and their accessibility. However, obstacles like poor cell survival and differentiation prevent them from being used in transplants. Thus, this study examined WJMSCs’ Conditioned Medium (CM) (WJMSCs-CM) for OA treatment. In order to determine how well IL1β-induced chondrocyte cells (IL1β-CHON002) heal OA-damaged cells, they were treated with both IGF1-induced and uninduced WJMSCs-CM. Key parameters measured were IL1β, Sox9 and COL2. The STRING database was explored to evaluate the proteins contained in WJMSCs-CM and to see their interactions and CM potential in OA exposure. The STRING database indicates WJMSCs-CM to possess the ability to manage OA because of the growth factors they contain, which raise the expression of cartilage marker genes. The results indicated that 15% WJMSCs-CM with 150 ng/mL IGF1 had the highest Sox9 expression, significant reduction in pro-inflammatory mediators, and an increase in COL2 levels. Additionally, 14 days of treatment resulted in better outcomes compared to 7 days. CM from WJMSCs treated with IGF1 with a concentration of 15% exhibited the highest level for each parameter. Both durations showed difference across parameters, highlighting the potential for OA therapy.
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| format | Article |
| id | doaj-art-7b8ff377c2d949b0949f336509fba3ab |
| institution | Kabale University |
| issn | 2337-5760 2338-5510 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | ITB Journal Publisher |
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| series | Journal of Mathematical and Fundamental Sciences |
| spelling | doaj-art-7b8ff377c2d949b0949f336509fba3ab2025-08-20T03:42:18ZengITB Journal PublisherJournal of Mathematical and Fundamental Sciences2337-57602338-55102024-12-0156310.5614/j.math.fund.sci.2024.56.3.1Conditioned Medium of IGF1-Induced Human Wharton’s Jelly Mesenchymal Stem Cells Effects on Sox9 Gene Expression and Level of COL2 and IL1β in Osteoarthritic ChondrocytesWahyu Widowati0Hanna Sari Widya Kusuma1Rizal Azis2The Fransiska Eltania3Faradhina Salfa Nindya4Faculty of Medicine, Maranatha Christian University, Jalan Surya Sumantri No. 65, Bandung, West Java 40164 IndonesiaBiomolecular and Biomedical Research Center, Aretha Medika Utama, Jalan Babakan Jeruk II No. 9, Bandung, West Java 40163 IndonesiaBiomolecular and Biomedical Research Center, Aretha Medika Utama, Jalan Babakan Jeruk II No. 9, Bandung, West Java 40163 IndonesiaFaculty of Medicine, Maranatha Christian University, Jalan Surya Sumantri No. 65, Bandung, West Java 40164 IndonesiaBiomolecular and Biomedical Research Center, Aretha Medika Utama, Jalan Babakan Jeruk II No. 9, Bandung, West Java 40163 Indonesia Wharton’s jelly mesenchymal stem cells (WJMSCs) hold promises for treating osteoarthritis (OA) due to their great capacity for regeneration and their accessibility. However, obstacles like poor cell survival and differentiation prevent them from being used in transplants. Thus, this study examined WJMSCs’ Conditioned Medium (CM) (WJMSCs-CM) for OA treatment. In order to determine how well IL1β-induced chondrocyte cells (IL1β-CHON002) heal OA-damaged cells, they were treated with both IGF1-induced and uninduced WJMSCs-CM. Key parameters measured were IL1β, Sox9 and COL2. The STRING database was explored to evaluate the proteins contained in WJMSCs-CM and to see their interactions and CM potential in OA exposure. The STRING database indicates WJMSCs-CM to possess the ability to manage OA because of the growth factors they contain, which raise the expression of cartilage marker genes. The results indicated that 15% WJMSCs-CM with 150 ng/mL IGF1 had the highest Sox9 expression, significant reduction in pro-inflammatory mediators, and an increase in COL2 levels. Additionally, 14 days of treatment resulted in better outcomes compared to 7 days. CM from WJMSCs treated with IGF1 with a concentration of 15% exhibited the highest level for each parameter. Both durations showed difference across parameters, highlighting the potential for OA therapy. http://167.205.195.146/ojsnew/index.php/jmfs/article/view/18326collagen 2IGF-1IL-1βsox9WJMSCs |
| spellingShingle | Wahyu Widowati Hanna Sari Widya Kusuma Rizal Azis The Fransiska Eltania Faradhina Salfa Nindya Conditioned Medium of IGF1-Induced Human Wharton’s Jelly Mesenchymal Stem Cells Effects on Sox9 Gene Expression and Level of COL2 and IL1β in Osteoarthritic Chondrocytes Journal of Mathematical and Fundamental Sciences collagen 2 IGF-1 IL-1β sox9 WJMSCs |
| title | Conditioned Medium of IGF1-Induced Human Wharton’s Jelly Mesenchymal Stem Cells Effects on Sox9 Gene Expression and Level of COL2 and IL1β in Osteoarthritic Chondrocytes |
| title_full | Conditioned Medium of IGF1-Induced Human Wharton’s Jelly Mesenchymal Stem Cells Effects on Sox9 Gene Expression and Level of COL2 and IL1β in Osteoarthritic Chondrocytes |
| title_fullStr | Conditioned Medium of IGF1-Induced Human Wharton’s Jelly Mesenchymal Stem Cells Effects on Sox9 Gene Expression and Level of COL2 and IL1β in Osteoarthritic Chondrocytes |
| title_full_unstemmed | Conditioned Medium of IGF1-Induced Human Wharton’s Jelly Mesenchymal Stem Cells Effects on Sox9 Gene Expression and Level of COL2 and IL1β in Osteoarthritic Chondrocytes |
| title_short | Conditioned Medium of IGF1-Induced Human Wharton’s Jelly Mesenchymal Stem Cells Effects on Sox9 Gene Expression and Level of COL2 and IL1β in Osteoarthritic Chondrocytes |
| title_sort | conditioned medium of igf1 induced human wharton s jelly mesenchymal stem cells effects on sox9 gene expression and level of col2 and il1β in osteoarthritic chondrocytes |
| topic | collagen 2 IGF-1 IL-1β sox9 WJMSCs |
| url | http://167.205.195.146/ojsnew/index.php/jmfs/article/view/18326 |
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