Improvement of the inflammation-damaged intestinal barrier and modulation of the gut microbiota in ulcerative colitis after FMT in the SHIME® model
Abstract Background Fecal microbiota transplantation (FMT) seems to be a promising approach in ulcerative colitis (UC) management with the aim of repopulating a patient’s dysbiotic microbiota with beneficial bacteria and restore its metabolic activity to its healthy characteristics. Metabolites pres...
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BMC
2025-04-01
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| Series: | BMC Complementary Medicine and Therapies |
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| Online Access: | https://doi.org/10.1186/s12906-025-04889-9 |
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| author | Anna Kamlárová Monika Kvaková Ľuboš Ambro René Link Izabela Bertková Zdenka Hertelyová Martin Janíčko Emília Hijová Jana Štofilová |
| author_facet | Anna Kamlárová Monika Kvaková Ľuboš Ambro René Link Izabela Bertková Zdenka Hertelyová Martin Janíčko Emília Hijová Jana Štofilová |
| author_sort | Anna Kamlárová |
| collection | DOAJ |
| description | Abstract Background Fecal microbiota transplantation (FMT) seems to be a promising approach in ulcerative colitis (UC) management with the aim of repopulating a patient’s dysbiotic microbiota with beneficial bacteria and restore its metabolic activity to its healthy characteristics. Metabolites present after FMT may improve the function and integrity of the intestinal barrier, reduce inflammation, and thus induce remission in an UC patient. In this study we evaluated whether the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®) model may be a suitable non-invasive alternative for studying and modifying the dysbiotic microbiota in UC by FMT application. Methods SHIME® model was used to investigate microbial and metabolic changes in the gut microbiota of UC patient induced by FMT application. FMT-modified metabolites from SHIME® were applied to an in vitro model of the intestinal barrier (differentiated Caco-2 and HT-29-MTX-E12 cell lines) compromised by pro-inflammatory cytokines to study the effect of FMT on the intestinal barrier. Results Qualitative and quantitative microbial analyses showed that FMT increased the diversity and variability of the microbiota in UC patient associated with a significant increase in total bacteria, Bacteroidota and Lactobacillus, as well as an increase in butyrate levels. In addition, an increase in the relative abundance of some important species such as Faecalibacterium prausnitzii and Bifidobacterium longum was observed, and there was also an enrichment of the microbiota with new species such as Blautia obeum, Roseburia faecis, Bifidobacterium adolescentis, Fusicatenibacter saccharivorans and Eubacterium rectale. Furthermore, microbial metabolites modulated by FMT from the SHIME® model prevented intestinal barrier damage and inhibited interleukin 8 (IL-8) and monocyte chemoattractant protein 1 (MCP-1) secretion when cell barriers were pretreated with FMT medium for 24 h. In summary, this study confirmed that a single dose of FMT beneficially modulated the composition and metabolic activity of the UC microbiota in the SHIME® model. Conclusions FMT favorably modulates the gut microbiota of UC patient cultured in the SHIME® model. FMT-modulated SHIME-derived microbial metabolites improve intact and inflamed intestinal barrier properties in vitro. Repeated applications are necessary to maintain the beneficial effect of FMT in SHIME® model. |
| format | Article |
| id | doaj-art-7b8719f5c0af401a9b24dc7769beeace |
| institution | OA Journals |
| issn | 2662-7671 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMC |
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| spelling | doaj-art-7b8719f5c0af401a9b24dc7769beeace2025-08-20T02:30:19ZengBMCBMC Complementary Medicine and Therapies2662-76712025-04-0125111910.1186/s12906-025-04889-9Improvement of the inflammation-damaged intestinal barrier and modulation of the gut microbiota in ulcerative colitis after FMT in the SHIME® modelAnna Kamlárová0Monika Kvaková1Ľuboš Ambro2René Link3Izabela Bertková4Zdenka Hertelyová5Martin Janíčko6Emília Hijová7Jana Štofilová8Center of Clinical and Preclinical Research – MediPark, Faculty of Medicine, P. J. Šafárik UniversityCenter of Clinical and Preclinical Research – MediPark, Faculty of Medicine, P. J. Šafárik UniversityCenter for Interdisciplinary Biosciences, Technology and Innovation Park, P.J. Šafárik UniversityCenter of Clinical and Preclinical Research – MediPark, Faculty of Medicine, P. J. Šafárik UniversityCenter of Clinical and Preclinical Research – MediPark, Faculty of Medicine, P. J. Šafárik UniversityCenter of Clinical and Preclinical Research – MediPark, Faculty of Medicine, P. J. Šafárik University2nd Department of Internal Medicine, Faculty of Medicine, P. J. Šafárik UniversityCenter of Clinical and Preclinical Research – MediPark, Faculty of Medicine, P. J. Šafárik UniversityCenter of Clinical and Preclinical Research – MediPark, Faculty of Medicine, P. J. Šafárik UniversityAbstract Background Fecal microbiota transplantation (FMT) seems to be a promising approach in ulcerative colitis (UC) management with the aim of repopulating a patient’s dysbiotic microbiota with beneficial bacteria and restore its metabolic activity to its healthy characteristics. Metabolites present after FMT may improve the function and integrity of the intestinal barrier, reduce inflammation, and thus induce remission in an UC patient. In this study we evaluated whether the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®) model may be a suitable non-invasive alternative for studying and modifying the dysbiotic microbiota in UC by FMT application. Methods SHIME® model was used to investigate microbial and metabolic changes in the gut microbiota of UC patient induced by FMT application. FMT-modified metabolites from SHIME® were applied to an in vitro model of the intestinal barrier (differentiated Caco-2 and HT-29-MTX-E12 cell lines) compromised by pro-inflammatory cytokines to study the effect of FMT on the intestinal barrier. Results Qualitative and quantitative microbial analyses showed that FMT increased the diversity and variability of the microbiota in UC patient associated with a significant increase in total bacteria, Bacteroidota and Lactobacillus, as well as an increase in butyrate levels. In addition, an increase in the relative abundance of some important species such as Faecalibacterium prausnitzii and Bifidobacterium longum was observed, and there was also an enrichment of the microbiota with new species such as Blautia obeum, Roseburia faecis, Bifidobacterium adolescentis, Fusicatenibacter saccharivorans and Eubacterium rectale. Furthermore, microbial metabolites modulated by FMT from the SHIME® model prevented intestinal barrier damage and inhibited interleukin 8 (IL-8) and monocyte chemoattractant protein 1 (MCP-1) secretion when cell barriers were pretreated with FMT medium for 24 h. In summary, this study confirmed that a single dose of FMT beneficially modulated the composition and metabolic activity of the UC microbiota in the SHIME® model. Conclusions FMT favorably modulates the gut microbiota of UC patient cultured in the SHIME® model. FMT-modulated SHIME-derived microbial metabolites improve intact and inflamed intestinal barrier properties in vitro. Repeated applications are necessary to maintain the beneficial effect of FMT in SHIME® model.https://doi.org/10.1186/s12906-025-04889-9Ulcerative colitisFecal microbiota transplantationDysbiosisIntestinal barrierInflammation |
| spellingShingle | Anna Kamlárová Monika Kvaková Ľuboš Ambro René Link Izabela Bertková Zdenka Hertelyová Martin Janíčko Emília Hijová Jana Štofilová Improvement of the inflammation-damaged intestinal barrier and modulation of the gut microbiota in ulcerative colitis after FMT in the SHIME® model BMC Complementary Medicine and Therapies Ulcerative colitis Fecal microbiota transplantation Dysbiosis Intestinal barrier Inflammation |
| title | Improvement of the inflammation-damaged intestinal barrier and modulation of the gut microbiota in ulcerative colitis after FMT in the SHIME® model |
| title_full | Improvement of the inflammation-damaged intestinal barrier and modulation of the gut microbiota in ulcerative colitis after FMT in the SHIME® model |
| title_fullStr | Improvement of the inflammation-damaged intestinal barrier and modulation of the gut microbiota in ulcerative colitis after FMT in the SHIME® model |
| title_full_unstemmed | Improvement of the inflammation-damaged intestinal barrier and modulation of the gut microbiota in ulcerative colitis after FMT in the SHIME® model |
| title_short | Improvement of the inflammation-damaged intestinal barrier and modulation of the gut microbiota in ulcerative colitis after FMT in the SHIME® model |
| title_sort | improvement of the inflammation damaged intestinal barrier and modulation of the gut microbiota in ulcerative colitis after fmt in the shime r model |
| topic | Ulcerative colitis Fecal microbiota transplantation Dysbiosis Intestinal barrier Inflammation |
| url | https://doi.org/10.1186/s12906-025-04889-9 |
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