Quantitative PCR genotyping assay for the Ts65Dn mouse model of Down syndrome
The Ts65Dn mouse is a segmentally trisomic model for Down syndrome. Until now, Ts65Dn mice have been identified by the laborious methods of either chromosomal analysis of cultured peripheral lymphocytes or fluorescent in situ hybridization (FISH). We report here a quantitative PCR method for genotyp...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2003-12-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/03356st02 |
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| _version_ | 1850152322349400064 |
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| author | Dong P. Liu Cecilia Schmidt Timothy Billings Muriel T. Davisson |
| author_facet | Dong P. Liu Cecilia Schmidt Timothy Billings Muriel T. Davisson |
| author_sort | Dong P. Liu |
| collection | DOAJ |
| description | The Ts65Dn mouse is a segmentally trisomic model for Down syndrome. Until now, Ts65Dn mice have been identified by the laborious methods of either chromosomal analysis of cultured peripheral lymphocytes or fluorescent in situ hybridization (FISH). We report here a quantitative PCR method for genotyping Ts65Dn mice, as well as a phenotypic description for visually preclassifying mice to be genotyped. |
| format | Article |
| id | doaj-art-7b76cca8f70b448b9e9a6cb3033d5d60 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2003-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-7b76cca8f70b448b9e9a6cb3033d5d602025-08-20T02:26:00ZengTaylor & Francis GroupBioTechniques0736-62051940-98182003-12-013561170118010.2144/03356st02Quantitative PCR genotyping assay for the Ts65Dn mouse model of Down syndromeDong P. Liu0Cecilia Schmidt1Timothy Billings2Muriel T. Davisson31The Jackson Laboratory, Bar Harbor, ME, USA1The Jackson Laboratory, Bar Harbor, ME, USA1The Jackson Laboratory, Bar Harbor, ME, USA1The Jackson Laboratory, Bar Harbor, ME, USAThe Ts65Dn mouse is a segmentally trisomic model for Down syndrome. Until now, Ts65Dn mice have been identified by the laborious methods of either chromosomal analysis of cultured peripheral lymphocytes or fluorescent in situ hybridization (FISH). We report here a quantitative PCR method for genotyping Ts65Dn mice, as well as a phenotypic description for visually preclassifying mice to be genotyped.https://www.future-science.com/doi/10.2144/03356st02 |
| spellingShingle | Dong P. Liu Cecilia Schmidt Timothy Billings Muriel T. Davisson Quantitative PCR genotyping assay for the Ts65Dn mouse model of Down syndrome BioTechniques |
| title | Quantitative PCR genotyping assay for the Ts65Dn mouse model of Down syndrome |
| title_full | Quantitative PCR genotyping assay for the Ts65Dn mouse model of Down syndrome |
| title_fullStr | Quantitative PCR genotyping assay for the Ts65Dn mouse model of Down syndrome |
| title_full_unstemmed | Quantitative PCR genotyping assay for the Ts65Dn mouse model of Down syndrome |
| title_short | Quantitative PCR genotyping assay for the Ts65Dn mouse model of Down syndrome |
| title_sort | quantitative pcr genotyping assay for the ts65dn mouse model of down syndrome |
| url | https://www.future-science.com/doi/10.2144/03356st02 |
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