Protocol for correlating the gut microbiome and metabolomics in patients with intracranial aneurysms
Summary: Gut-microbiome-combined metabolomics studies in cerebrovascular disease highlight the microbiota-gut-brain axis in neurological disorders. Here, we present a protocol for correlating the gut microbiome and metabolomics in patients with intracranial aneurysms. We describe steps for sample co...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724007470 |
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| author | Hongyu Xu Zetian Jia Junhui Liu Runming Liu Wei Wei Xiang Li |
| author_facet | Hongyu Xu Zetian Jia Junhui Liu Runming Liu Wei Wei Xiang Li |
| author_sort | Hongyu Xu |
| collection | DOAJ |
| description | Summary: Gut-microbiome-combined metabolomics studies in cerebrovascular disease highlight the microbiota-gut-brain axis in neurological disorders. Here, we present a protocol for correlating the gut microbiome and metabolomics in patients with intracranial aneurysms. We describe steps for sample collection, fecal genomic DNA extraction, rRNA PCR amplification, sequencing library construction, and rRNA sequencing. We then detail procedures for metabolite extraction, liquid chromatography-tandem mass spectrometry (LC-MS/MS) non-targeted metabolomics sequencing, and ELISA for cerebrospinal fluid and plasma samples. Finally, we perform combined multi-omics analysis.For complete details on the use and execution of this protocol, please refer to Xu et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-7b6ecd4aa3004120aba22e4642339314 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-7b6ecd4aa3004120aba22e46423393142025-01-23T05:27:45ZengElsevierSTAR Protocols2666-16672025-03-0161103582Protocol for correlating the gut microbiome and metabolomics in patients with intracranial aneurysmsHongyu Xu0Zetian Jia1Junhui Liu2Runming Liu3Wei Wei4Xiang Li5Brain Research Center, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, China; Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, China; Corresponding authorBrain Research Center, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, China; Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, ChinaBrain Research Center, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, China; Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, ChinaBrain Research Center, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, China; Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, ChinaBrain Research Center, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, China; Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, China; Corresponding authorBrain Research Center, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, China; Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, China; Frontier Science Center for Immunology and Metabolism, Wuhan University, Wuhan 430072, China; Medical Research Institute, Wuhan University, Wuhan 430072, China; Sino-Italian Ascula Brain Science Joint Laboratory, Wuhan University, Wuhan 430071, China; Corresponding authorSummary: Gut-microbiome-combined metabolomics studies in cerebrovascular disease highlight the microbiota-gut-brain axis in neurological disorders. Here, we present a protocol for correlating the gut microbiome and metabolomics in patients with intracranial aneurysms. We describe steps for sample collection, fecal genomic DNA extraction, rRNA PCR amplification, sequencing library construction, and rRNA sequencing. We then detail procedures for metabolite extraction, liquid chromatography-tandem mass spectrometry (LC-MS/MS) non-targeted metabolomics sequencing, and ELISA for cerebrospinal fluid and plasma samples. Finally, we perform combined multi-omics analysis.For complete details on the use and execution of this protocol, please refer to Xu et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724007470Clinical ProtocolSequencingMetabolomicsMicrobiology |
| spellingShingle | Hongyu Xu Zetian Jia Junhui Liu Runming Liu Wei Wei Xiang Li Protocol for correlating the gut microbiome and metabolomics in patients with intracranial aneurysms STAR Protocols Clinical Protocol Sequencing Metabolomics Microbiology |
| title | Protocol for correlating the gut microbiome and metabolomics in patients with intracranial aneurysms |
| title_full | Protocol for correlating the gut microbiome and metabolomics in patients with intracranial aneurysms |
| title_fullStr | Protocol for correlating the gut microbiome and metabolomics in patients with intracranial aneurysms |
| title_full_unstemmed | Protocol for correlating the gut microbiome and metabolomics in patients with intracranial aneurysms |
| title_short | Protocol for correlating the gut microbiome and metabolomics in patients with intracranial aneurysms |
| title_sort | protocol for correlating the gut microbiome and metabolomics in patients with intracranial aneurysms |
| topic | Clinical Protocol Sequencing Metabolomics Microbiology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724007470 |
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