Continuous Nondestructive Monitoring Method Using the Reconstructed Three-Dimensional Conductivity Images via GREIT for Tissue Engineering

A continuous Nondestructive monitoring method is required to apply proper feedback controls during tissue regeneration. Conductivity is one of valuable information to assess the physiological function and structural formation of regenerated tissues or cultured cells. However, conductivity imaging me...

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Main Authors: Sujin Ahn, Hun Wi, Tong In Oh, Alistair Lee McEwan, Sung Chan Jun, Eung Je Woo
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:Journal of Applied Mathematics
Online Access:http://dx.doi.org/10.1155/2014/562176
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author Sujin Ahn
Hun Wi
Tong In Oh
Alistair Lee McEwan
Sung Chan Jun
Eung Je Woo
author_facet Sujin Ahn
Hun Wi
Tong In Oh
Alistair Lee McEwan
Sung Chan Jun
Eung Je Woo
author_sort Sujin Ahn
collection DOAJ
description A continuous Nondestructive monitoring method is required to apply proper feedback controls during tissue regeneration. Conductivity is one of valuable information to assess the physiological function and structural formation of regenerated tissues or cultured cells. However, conductivity imaging methods suffered from inherited ill-posed characteristics in image reconstruction, unknown boundary geometry, uncertainty in electrode position, and systematic artifacts. In order to overcome the limitation of microscopic electrical impedance tomography (micro-EIT), we applied a 3D-specific container with a fixed boundary geometry and electrode configuration to maximize the performance of Graz consensus reconstruction algorithm for EIT (GREIT). The separation of driving and sensing electrodes allows us to simplify the hardware complexity and obtain higher measurement accuracy from a large number of small sensing electrodes. We investigated the applicability of the GREIT to 3D micro-EIT images via numerical simulations and large-scale phantom experiments. We could reconstruct multiple objects regardless of the location. The resolution was 5 mm3 with 30 dB SNR and the position error was less than 2.54 mm. This shows that the new micro-EIT system integrated with GREIT is robust with the intended resolution. With further refinement and scaling down to a microscale container, it may be a continuous nondestructive monitoring tool for tissue engineering applications.
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institution Kabale University
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spelling doaj-art-7b6e7bfcdcba4b52bfb2ab2d74e6b4ab2025-08-20T03:35:02ZengWileyJournal of Applied Mathematics1110-757X1687-00422014-01-01201410.1155/2014/562176562176Continuous Nondestructive Monitoring Method Using the Reconstructed Three-Dimensional Conductivity Images via GREIT for Tissue EngineeringSujin Ahn0Hun Wi1Tong In Oh2Alistair Lee McEwan3Sung Chan Jun4Eung Je Woo5School of Information & Communications, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of KoreaImpedance Imaging Research Center and Department of Biomedical Engineering, Kyung Hee University, Yongin 446-701, Republic of KoreaImpedance Imaging Research Center and Department of Biomedical Engineering, Kyung Hee University, Yongin 446-701, Republic of KoreaImpedance Imaging Research Center and Department of Biomedical Engineering, Kyung Hee University, Yongin 446-701, Republic of KoreaSchool of Information & Communications, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of KoreaImpedance Imaging Research Center and Department of Biomedical Engineering, Kyung Hee University, Yongin 446-701, Republic of KoreaA continuous Nondestructive monitoring method is required to apply proper feedback controls during tissue regeneration. Conductivity is one of valuable information to assess the physiological function and structural formation of regenerated tissues or cultured cells. However, conductivity imaging methods suffered from inherited ill-posed characteristics in image reconstruction, unknown boundary geometry, uncertainty in electrode position, and systematic artifacts. In order to overcome the limitation of microscopic electrical impedance tomography (micro-EIT), we applied a 3D-specific container with a fixed boundary geometry and electrode configuration to maximize the performance of Graz consensus reconstruction algorithm for EIT (GREIT). The separation of driving and sensing electrodes allows us to simplify the hardware complexity and obtain higher measurement accuracy from a large number of small sensing electrodes. We investigated the applicability of the GREIT to 3D micro-EIT images via numerical simulations and large-scale phantom experiments. We could reconstruct multiple objects regardless of the location. The resolution was 5 mm3 with 30 dB SNR and the position error was less than 2.54 mm. This shows that the new micro-EIT system integrated with GREIT is robust with the intended resolution. With further refinement and scaling down to a microscale container, it may be a continuous nondestructive monitoring tool for tissue engineering applications.http://dx.doi.org/10.1155/2014/562176
spellingShingle Sujin Ahn
Hun Wi
Tong In Oh
Alistair Lee McEwan
Sung Chan Jun
Eung Je Woo
Continuous Nondestructive Monitoring Method Using the Reconstructed Three-Dimensional Conductivity Images via GREIT for Tissue Engineering
Journal of Applied Mathematics
title Continuous Nondestructive Monitoring Method Using the Reconstructed Three-Dimensional Conductivity Images via GREIT for Tissue Engineering
title_full Continuous Nondestructive Monitoring Method Using the Reconstructed Three-Dimensional Conductivity Images via GREIT for Tissue Engineering
title_fullStr Continuous Nondestructive Monitoring Method Using the Reconstructed Three-Dimensional Conductivity Images via GREIT for Tissue Engineering
title_full_unstemmed Continuous Nondestructive Monitoring Method Using the Reconstructed Three-Dimensional Conductivity Images via GREIT for Tissue Engineering
title_short Continuous Nondestructive Monitoring Method Using the Reconstructed Three-Dimensional Conductivity Images via GREIT for Tissue Engineering
title_sort continuous nondestructive monitoring method using the reconstructed three dimensional conductivity images via greit for tissue engineering
url http://dx.doi.org/10.1155/2014/562176
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