Comparative evaluation of Gingival Crevicular Fluid (GCF) levels of Interleukin-34 levels in periodontally healthy and in patients with chronic and aggressive periodontitis- A cross-sectional study
Background and objectives: Proinflammatory cytokines appear to have a central role in the destruction of periodontal tissues. By binding to Receptor Activator of Nuclear Factor κb (RANK) on osteoclast progenitor cells, these cytokines are locally responsible for the activation of bone resorbing oste...
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Springer
2019-07-01
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| Series: | Saudi Dental Journal |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1013905218305121 |
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| author | Parichaya Batra Sushma Das Poonam Patel |
| author_facet | Parichaya Batra Sushma Das Poonam Patel |
| author_sort | Parichaya Batra |
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| description | Background and objectives: Proinflammatory cytokines appear to have a central role in the destruction of periodontal tissues. By binding to Receptor Activator of Nuclear Factor κb (RANK) on osteoclast progenitor cells, these cytokines are locally responsible for the activation of bone resorbing osteoclasts differentiation and function. Interleukin-34 (IL-34) is a proinflammatory cytokine derived from the osteoblasts and plays an important role in osteoclastogenesis. The present study was carried out to assess the levels of IL-34 in Gingival Crevicular Fluid (GCF) in periodontally healthy patients, chronic periodontitis and aggressive periodontitis. Materials and method: A total of 90 patients were recruited in the study and were divided into 3 groups: Periodontally healthy patients (Group I), Chronic periodontitis (Group II) and Aggressive periodontitis (Group III). Clinical Parameters like plaque index, gingival index, probing depth, clinical attachment levels were recorded and GCF samples were collected at baseline for the assessment of IL-34 levels in all the three groups. IL-34 levels were calculated using Enzyme-Linked Immune-Sorbent Assay (ELISA). Results: The mean GCF levels of IL-34 in Group I was 47.22 ± 18.04 pg/ml, Group II was 103.76 ± 26.61 pg/ml and in Group III was 191.71 ± 49.24 pg/ml. The mean GCF IL-34 level was found to be higher in Group III followed by Group II and Group I. The IL-34 levels correlated with clinical parameters like plaque index, gingival index, probing depth, clinical attachment levels. But correlation with probing depth and clinical attachment level were significantly higher in Group III followed by Group II and Group I. Interpretation and conclusion: The study demonstrated higher levels of IL-34 in aggressive periodontitis when compared with chronic periodontitis. IL-34, a novel diagnostic marker which was found to be at higher level in GCF of aggressive and chronic periodontitis patients. Keywords: Interleukin-34, Aggressive periodontitis, Chronic periodontitis, Cytokine |
| format | Article |
| id | doaj-art-7b61b928bc3344b2bc96bf99f2c2bb04 |
| institution | OA Journals |
| issn | 1013-9052 |
| language | English |
| publishDate | 2019-07-01 |
| publisher | Springer |
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| series | Saudi Dental Journal |
| spelling | doaj-art-7b61b928bc3344b2bc96bf99f2c2bb042025-08-20T02:07:03ZengSpringerSaudi Dental Journal1013-90522019-07-0131331632110.1016/j.sdentj.2019.03.010Comparative evaluation of Gingival Crevicular Fluid (GCF) levels of Interleukin-34 levels in periodontally healthy and in patients with chronic and aggressive periodontitis- A cross-sectional studyParichaya Batra0Sushma Das1Poonam Patel2The Department of Periodontics, The Oxford Dental College, Bommanahalli, Bangalore, India; Corresponding author at: The Department of Periodontics, Vydehi Institute of Dental Sciences & Research Centre, No. 82, EPIP Area, Nallurahalli, Whitefield, Bangalore 560066, India.The Department of Periodontics, The Oxford Dental College, Bommanahalli, Bangalore, IndiaThe Department of Public Health Dentistry, Vydehi Institute of Dental Sciences & Research Centre, No. 82, EPIP Area, Nallurahalli, Whitefield, Bangalore 560066, IndiaBackground and objectives: Proinflammatory cytokines appear to have a central role in the destruction of periodontal tissues. By binding to Receptor Activator of Nuclear Factor κb (RANK) on osteoclast progenitor cells, these cytokines are locally responsible for the activation of bone resorbing osteoclasts differentiation and function. Interleukin-34 (IL-34) is a proinflammatory cytokine derived from the osteoblasts and plays an important role in osteoclastogenesis. The present study was carried out to assess the levels of IL-34 in Gingival Crevicular Fluid (GCF) in periodontally healthy patients, chronic periodontitis and aggressive periodontitis. Materials and method: A total of 90 patients were recruited in the study and were divided into 3 groups: Periodontally healthy patients (Group I), Chronic periodontitis (Group II) and Aggressive periodontitis (Group III). Clinical Parameters like plaque index, gingival index, probing depth, clinical attachment levels were recorded and GCF samples were collected at baseline for the assessment of IL-34 levels in all the three groups. IL-34 levels were calculated using Enzyme-Linked Immune-Sorbent Assay (ELISA). Results: The mean GCF levels of IL-34 in Group I was 47.22 ± 18.04 pg/ml, Group II was 103.76 ± 26.61 pg/ml and in Group III was 191.71 ± 49.24 pg/ml. The mean GCF IL-34 level was found to be higher in Group III followed by Group II and Group I. The IL-34 levels correlated with clinical parameters like plaque index, gingival index, probing depth, clinical attachment levels. But correlation with probing depth and clinical attachment level were significantly higher in Group III followed by Group II and Group I. Interpretation and conclusion: The study demonstrated higher levels of IL-34 in aggressive periodontitis when compared with chronic periodontitis. IL-34, a novel diagnostic marker which was found to be at higher level in GCF of aggressive and chronic periodontitis patients. Keywords: Interleukin-34, Aggressive periodontitis, Chronic periodontitis, Cytokinehttp://www.sciencedirect.com/science/article/pii/S1013905218305121 |
| spellingShingle | Parichaya Batra Sushma Das Poonam Patel Comparative evaluation of Gingival Crevicular Fluid (GCF) levels of Interleukin-34 levels in periodontally healthy and in patients with chronic and aggressive periodontitis- A cross-sectional study Saudi Dental Journal |
| title | Comparative evaluation of Gingival Crevicular Fluid (GCF) levels of Interleukin-34 levels in periodontally healthy and in patients with chronic and aggressive periodontitis- A cross-sectional study |
| title_full | Comparative evaluation of Gingival Crevicular Fluid (GCF) levels of Interleukin-34 levels in periodontally healthy and in patients with chronic and aggressive periodontitis- A cross-sectional study |
| title_fullStr | Comparative evaluation of Gingival Crevicular Fluid (GCF) levels of Interleukin-34 levels in periodontally healthy and in patients with chronic and aggressive periodontitis- A cross-sectional study |
| title_full_unstemmed | Comparative evaluation of Gingival Crevicular Fluid (GCF) levels of Interleukin-34 levels in periodontally healthy and in patients with chronic and aggressive periodontitis- A cross-sectional study |
| title_short | Comparative evaluation of Gingival Crevicular Fluid (GCF) levels of Interleukin-34 levels in periodontally healthy and in patients with chronic and aggressive periodontitis- A cross-sectional study |
| title_sort | comparative evaluation of gingival crevicular fluid gcf levels of interleukin 34 levels in periodontally healthy and in patients with chronic and aggressive periodontitis a cross sectional study |
| url | http://www.sciencedirect.com/science/article/pii/S1013905218305121 |
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