Dietary Genistein Influences Number of Acetylcholine Receptors in Female Diabetic Jejunum
Background. Intestinal dysfunction in the ob/ob mouse model of diabetes mimics that seen clinically. Methods. We determined the effects of a 4-week genistein diet (600 mg genistein/kg food) on intestinal function (contractility, morphology, AChR, and motility) in female ob/ob and lean mice. Results....
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2017/3568146 |
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| author | Sydney Schacht Faisal Masood Shawn Catmull Robert Dolan RussL Altabtabaee Wade Grow Layla Al-Nakkash |
| author_facet | Sydney Schacht Faisal Masood Shawn Catmull Robert Dolan RussL Altabtabaee Wade Grow Layla Al-Nakkash |
| author_sort | Sydney Schacht |
| collection | DOAJ |
| description | Background. Intestinal dysfunction in the ob/ob mouse model of diabetes mimics that seen clinically. Methods. We determined the effects of a 4-week genistein diet (600 mg genistein/kg food) on intestinal function (contractility, morphology, AChR, and motility) in female ob/ob and lean mice. Results. Contractility of the jejunum in response to incrementally increasing concentrations of KCl was comparable in ob/ob females and lean controls regardless of a genistein-diet. There were no changes in the wall thickness measured. We assessed the number of clusters of AChR in the jejunum wall; AChR were decreased by 48% in ob/ob mice versus leans, and the genistein diet reversed this. In utilizing a video-imaging system to evaluate gastrointestinal motility, we determined that the distance between consecutive contractile events was significantly increased by 1.87-fold in ob/ob mice versus leans, and the genistein diet was without effect. Conclusions. These data suggest that slowed intestinal transit in the diabetic ob/ob mouse may be due in part to decreased AChR and decreased contraction events occurring per unit time. A genistein diet rescues the number of AChR to levels of leans yet did not change the number of contractile events. Feeding ob/ob mice a genistein-rich diet has potential therapeutic benefits towards improving the debilitating diabetes-related gastrointestinal dysfunction. |
| format | Article |
| id | doaj-art-7b595f9d15bf48348be3297f6639a2c1 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-7b595f9d15bf48348be3297f6639a2c12025-08-20T03:35:03ZengWileyJournal of Diabetes Research2314-67452314-67532017-01-01201710.1155/2017/35681463568146Dietary Genistein Influences Number of Acetylcholine Receptors in Female Diabetic JejunumSydney Schacht0Faisal Masood1Shawn Catmull2Robert Dolan3RussL Altabtabaee4Wade Grow5Layla Al-Nakkash6Department of Physiology, Midwestern University, 19555 N. 59th Avenue, Glendale, AZ 85308, USADepartment of Physiology, Midwestern University, 19555 N. 59th Avenue, Glendale, AZ 85308, USADepartment of Physiology, Midwestern University, 19555 N. 59th Avenue, Glendale, AZ 85308, USADepartment of Physiology, Midwestern University, 19555 N. 59th Avenue, Glendale, AZ 85308, USADepartment of Physiology, Midwestern University, 19555 N. 59th Avenue, Glendale, AZ 85308, USADepartment of Anatomy, Arizona College of Osteopathic Medicine, Midwestern University, 19555 N. 59th Avenue, Glendale, AZ 85308, USADepartment of Physiology, Midwestern University, 19555 N. 59th Avenue, Glendale, AZ 85308, USABackground. Intestinal dysfunction in the ob/ob mouse model of diabetes mimics that seen clinically. Methods. We determined the effects of a 4-week genistein diet (600 mg genistein/kg food) on intestinal function (contractility, morphology, AChR, and motility) in female ob/ob and lean mice. Results. Contractility of the jejunum in response to incrementally increasing concentrations of KCl was comparable in ob/ob females and lean controls regardless of a genistein-diet. There were no changes in the wall thickness measured. We assessed the number of clusters of AChR in the jejunum wall; AChR were decreased by 48% in ob/ob mice versus leans, and the genistein diet reversed this. In utilizing a video-imaging system to evaluate gastrointestinal motility, we determined that the distance between consecutive contractile events was significantly increased by 1.87-fold in ob/ob mice versus leans, and the genistein diet was without effect. Conclusions. These data suggest that slowed intestinal transit in the diabetic ob/ob mouse may be due in part to decreased AChR and decreased contraction events occurring per unit time. A genistein diet rescues the number of AChR to levels of leans yet did not change the number of contractile events. Feeding ob/ob mice a genistein-rich diet has potential therapeutic benefits towards improving the debilitating diabetes-related gastrointestinal dysfunction.http://dx.doi.org/10.1155/2017/3568146 |
| spellingShingle | Sydney Schacht Faisal Masood Shawn Catmull Robert Dolan RussL Altabtabaee Wade Grow Layla Al-Nakkash Dietary Genistein Influences Number of Acetylcholine Receptors in Female Diabetic Jejunum Journal of Diabetes Research |
| title | Dietary Genistein Influences Number of Acetylcholine Receptors in Female Diabetic Jejunum |
| title_full | Dietary Genistein Influences Number of Acetylcholine Receptors in Female Diabetic Jejunum |
| title_fullStr | Dietary Genistein Influences Number of Acetylcholine Receptors in Female Diabetic Jejunum |
| title_full_unstemmed | Dietary Genistein Influences Number of Acetylcholine Receptors in Female Diabetic Jejunum |
| title_short | Dietary Genistein Influences Number of Acetylcholine Receptors in Female Diabetic Jejunum |
| title_sort | dietary genistein influences number of acetylcholine receptors in female diabetic jejunum |
| url | http://dx.doi.org/10.1155/2017/3568146 |
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