Phage Cocktail Alleviates Bacterial Canker of Kiwifruit by Modulating Bacterial Community Structure in Field Trial
Bacterial canker of kiwifruit is the most destructive bacterial disease caused by <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>. Bacteriophages are regarded as promising biocontrol agents against kiwifruit bacterial pathogens due to their exceptional host specificity and...
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2025-01-01
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| author | Ran Hu Xiaohan Xu Yajun Jia Cancan Zhu Lin Wang Minxin Song Qian Xu Mian Xia Xiaoqing He Yi Jin |
| author_facet | Ran Hu Xiaohan Xu Yajun Jia Cancan Zhu Lin Wang Minxin Song Qian Xu Mian Xia Xiaoqing He Yi Jin |
| author_sort | Ran Hu |
| collection | DOAJ |
| description | Bacterial canker of kiwifruit is the most destructive bacterial disease caused by <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>. Bacteriophages are regarded as promising biocontrol agents against kiwifruit bacterial pathogens due to their exceptional host specificity and environmentally friendly nature. However, the underlying mechanism of phages in the control of kiwifruit bacterial canker disease remains elusive. In this study, the field trial results showed that phage cocktail could significantly reduce the incidence of bacterial canker in kiwifruit. The high throughput sequencing results showed that the phage cocktail regulated the impact of pathogen invasion on branch endophytic communities, adjusted the diversity of the bacterial community structure, regulated the composition of rare taxa and abundant taxa, and increased the proportion of deterministic processes in community assembly processes. The phage cocktail significantly reduced the relative abundance of <i>Pseudomonadaceae</i>, <i>Pectobacteriaceae</i>, and <i>Yersiniacea</i>. Furthermore, the application of the phage cocktail resulted in an increase in the relative abundance of <i>Beijerinckiaceae</i>, <i>Sphingomonadaceae</i>, and <i>Xanthomonadaceae</i>, most of which are abundant taxa of the corresponding microbial communities. Additionally, the composition of rare taxa was also altered under the influence of phages. These findings offer perspectives on the phage-mediated biocontrol of kiwifruit bacterial canker and provide practical backing for the implementation of phage cocktails in sustainable agriculture. |
| format | Article |
| id | doaj-art-7b4fe3703384431da3dd1452458b6a65 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-7b4fe3703384431da3dd1452458b6a652025-01-24T13:42:40ZengMDPI AGMicroorganisms2076-26072025-01-0113110410.3390/microorganisms13010104Phage Cocktail Alleviates Bacterial Canker of Kiwifruit by Modulating Bacterial Community Structure in Field TrialRan Hu0Xiaohan Xu1Yajun Jia2Cancan Zhu3Lin Wang4Minxin Song5Qian Xu6Mian Xia7Xiaoqing He8Yi Jin9College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, ChinaHainan Yazhou Bay Seed Laboratory, Yazhou 572024, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, ChinaHainan Yazhou Bay Seed Laboratory, Yazhou 572024, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, ChinaBacterial canker of kiwifruit is the most destructive bacterial disease caused by <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>. Bacteriophages are regarded as promising biocontrol agents against kiwifruit bacterial pathogens due to their exceptional host specificity and environmentally friendly nature. However, the underlying mechanism of phages in the control of kiwifruit bacterial canker disease remains elusive. In this study, the field trial results showed that phage cocktail could significantly reduce the incidence of bacterial canker in kiwifruit. The high throughput sequencing results showed that the phage cocktail regulated the impact of pathogen invasion on branch endophytic communities, adjusted the diversity of the bacterial community structure, regulated the composition of rare taxa and abundant taxa, and increased the proportion of deterministic processes in community assembly processes. The phage cocktail significantly reduced the relative abundance of <i>Pseudomonadaceae</i>, <i>Pectobacteriaceae</i>, and <i>Yersiniacea</i>. Furthermore, the application of the phage cocktail resulted in an increase in the relative abundance of <i>Beijerinckiaceae</i>, <i>Sphingomonadaceae</i>, and <i>Xanthomonadaceae</i>, most of which are abundant taxa of the corresponding microbial communities. Additionally, the composition of rare taxa was also altered under the influence of phages. These findings offer perspectives on the phage-mediated biocontrol of kiwifruit bacterial canker and provide practical backing for the implementation of phage cocktails in sustainable agriculture.https://www.mdpi.com/2076-2607/13/1/104phage biocontrol<i>Pseudomonas syringae</i> pv. <i>actinidiae</i>endophytic microbiomecommunity assembly processes |
| spellingShingle | Ran Hu Xiaohan Xu Yajun Jia Cancan Zhu Lin Wang Minxin Song Qian Xu Mian Xia Xiaoqing He Yi Jin Phage Cocktail Alleviates Bacterial Canker of Kiwifruit by Modulating Bacterial Community Structure in Field Trial Microorganisms phage biocontrol <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> endophytic microbiome community assembly processes |
| title | Phage Cocktail Alleviates Bacterial Canker of Kiwifruit by Modulating Bacterial Community Structure in Field Trial |
| title_full | Phage Cocktail Alleviates Bacterial Canker of Kiwifruit by Modulating Bacterial Community Structure in Field Trial |
| title_fullStr | Phage Cocktail Alleviates Bacterial Canker of Kiwifruit by Modulating Bacterial Community Structure in Field Trial |
| title_full_unstemmed | Phage Cocktail Alleviates Bacterial Canker of Kiwifruit by Modulating Bacterial Community Structure in Field Trial |
| title_short | Phage Cocktail Alleviates Bacterial Canker of Kiwifruit by Modulating Bacterial Community Structure in Field Trial |
| title_sort | phage cocktail alleviates bacterial canker of kiwifruit by modulating bacterial community structure in field trial |
| topic | phage biocontrol <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> endophytic microbiome community assembly processes |
| url | https://www.mdpi.com/2076-2607/13/1/104 |
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