Can CAPRIN-1 Be Responsible for the Recurrence Potential of Odontogenic Keratocysts?
Objectives: The purpose of this retrospective study is to compare dentigerous cysts and odontogenic keratocysts for cytoplasmic activation/proliferation - associated protein-1 antibodies via immunohistochemical staining to obtain a new perspective about the specific behavioural characteristics of od...
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Lithuanian University of Health Sciences, Faculty of Odontology
2024-06-01
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| Series: | eJournal of Oral Maxillofacial Research |
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| Online Access: | https://www.ejomr.org/JOMR/archives/2024/2/e4/v15n2e4ht.htm |
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| author | Nelli Yildirimyan Mehmet Ali Altay İrem Hicran Özbudak |
| author_facet | Nelli Yildirimyan Mehmet Ali Altay İrem Hicran Özbudak |
| author_sort | Nelli Yildirimyan |
| collection | DOAJ |
| description | Objectives: The purpose of this retrospective study is to compare dentigerous cysts and odontogenic keratocysts for cytoplasmic activation/proliferation - associated protein-1 antibodies via immunohistochemical staining to obtain a new perspective about the specific behavioural characteristics of odontogenic keratocysts at the molecular level.
Material and Methods: Forty dentigerous cysts (DC) and forty odontogenic keratocysts (OKC) tissue samples were examined using immunohistochemical staining to detect cytoplasmic activation/proliferation - associated protein-1 (CAPRIN-1) antibodies. Nuclear and/or cytoplasmic staining was evaluated as “positive”. Cell staining rate (%) and cell staining intensity were determined, and a staining intensity distribution (SID) score was calculated for each sample. Cases were considered “negative” if they showed no staining for CAPRIN-1 antibodies, thus were given a SID score of zero. According to the SID scores, the expression levels were rated as negative, mild, moderate, or high.
Results: Of 80 samples, 16 that could adversely affect immunohistochemical evaluation were excluded. Ten negative, 21 positive and three negative, 30 positive CAPRIN-1 expressions were observed in DC and OKC groups, respectively. The difference between the negative and positive cases within groups was significant only in the OKC group (P = 0.000). The SID score range and mean were 0 to 160 and 31.1 (SD 35.7) for DC and 0 to 160 and 57.3 (SD 42.3) for OKC groups. CAPRIN-1 expression was significantly higher in the OKC group (P = 0.043).
Conclusions: The molecular basis for increased mitotic activity, high recurrence rates or presence of satellite cysts in odontogenic keratocysts may be attributed to the expression of cytoplasmic activation/proliferation - associated protein-1. |
| format | Article |
| id | doaj-art-7b4fc84fbbb44ccd815ded8fc720e628 |
| institution | DOAJ |
| issn | 2029-283X |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Lithuanian University of Health Sciences, Faculty of Odontology |
| record_format | Article |
| series | eJournal of Oral Maxillofacial Research |
| spelling | doaj-art-7b4fc84fbbb44ccd815ded8fc720e6282025-08-20T03:14:58ZengLithuanian University of Health Sciences, Faculty of OdontologyeJournal of Oral Maxillofacial Research2029-283X2024-06-01152e410.5037/jomr.2024.15204Can CAPRIN-1 Be Responsible for the Recurrence Potential of Odontogenic Keratocysts?Nelli YildirimyanMehmet Ali Altayİrem Hicran ÖzbudakObjectives: The purpose of this retrospective study is to compare dentigerous cysts and odontogenic keratocysts for cytoplasmic activation/proliferation - associated protein-1 antibodies via immunohistochemical staining to obtain a new perspective about the specific behavioural characteristics of odontogenic keratocysts at the molecular level. Material and Methods: Forty dentigerous cysts (DC) and forty odontogenic keratocysts (OKC) tissue samples were examined using immunohistochemical staining to detect cytoplasmic activation/proliferation - associated protein-1 (CAPRIN-1) antibodies. Nuclear and/or cytoplasmic staining was evaluated as “positive”. Cell staining rate (%) and cell staining intensity were determined, and a staining intensity distribution (SID) score was calculated for each sample. Cases were considered “negative” if they showed no staining for CAPRIN-1 antibodies, thus were given a SID score of zero. According to the SID scores, the expression levels were rated as negative, mild, moderate, or high. Results: Of 80 samples, 16 that could adversely affect immunohistochemical evaluation were excluded. Ten negative, 21 positive and three negative, 30 positive CAPRIN-1 expressions were observed in DC and OKC groups, respectively. The difference between the negative and positive cases within groups was significant only in the OKC group (P = 0.000). The SID score range and mean were 0 to 160 and 31.1 (SD 35.7) for DC and 0 to 160 and 57.3 (SD 42.3) for OKC groups. CAPRIN-1 expression was significantly higher in the OKC group (P = 0.043). Conclusions: The molecular basis for increased mitotic activity, high recurrence rates or presence of satellite cysts in odontogenic keratocysts may be attributed to the expression of cytoplasmic activation/proliferation - associated protein-1.https://www.ejomr.org/JOMR/archives/2024/2/e4/v15n2e4ht.htmcell proliferationdentigerous cystjaw cystsodontogenic cystsrecurrence |
| spellingShingle | Nelli Yildirimyan Mehmet Ali Altay İrem Hicran Özbudak Can CAPRIN-1 Be Responsible for the Recurrence Potential of Odontogenic Keratocysts? eJournal of Oral Maxillofacial Research cell proliferation dentigerous cyst jaw cysts odontogenic cysts recurrence |
| title | Can CAPRIN-1 Be Responsible for the Recurrence Potential of Odontogenic Keratocysts? |
| title_full | Can CAPRIN-1 Be Responsible for the Recurrence Potential of Odontogenic Keratocysts? |
| title_fullStr | Can CAPRIN-1 Be Responsible for the Recurrence Potential of Odontogenic Keratocysts? |
| title_full_unstemmed | Can CAPRIN-1 Be Responsible for the Recurrence Potential of Odontogenic Keratocysts? |
| title_short | Can CAPRIN-1 Be Responsible for the Recurrence Potential of Odontogenic Keratocysts? |
| title_sort | can caprin 1 be responsible for the recurrence potential of odontogenic keratocysts |
| topic | cell proliferation dentigerous cyst jaw cysts odontogenic cysts recurrence |
| url | https://www.ejomr.org/JOMR/archives/2024/2/e4/v15n2e4ht.htm |
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