Effects of <i>trans</i> n-6 fatty acids on the fatty acid profile of tissues and liver microsomal desaturation in the rat
18:2Δ 9c,12t and 18:2 Δ9t,12c are present in our diet, as result of heat treatment of vegetable oils. A nutritional study was carried out in order to obtain more precise information on the conversion of these two isomers into long chain polyunsaturated fatty acids (PUFA) by rat tissues. This in viv...
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Consejo Superior de Investigaciones Científicas
1996-04-01
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| Series: | Grasas y Aceites |
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| Online Access: | https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/849 |
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| author | Olivier Berdeaux Jean Louis Sébédio Jean Michel Chardigny Jean Paul Blond Thierry Mairot Jean Michel Vatèles Didier Poullain Jean Pierre Nöel |
| author_facet | Olivier Berdeaux Jean Louis Sébédio Jean Michel Chardigny Jean Paul Blond Thierry Mairot Jean Michel Vatèles Didier Poullain Jean Pierre Nöel |
| author_sort | Olivier Berdeaux |
| collection | DOAJ |
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18:2Δ 9c,12t and 18:2 Δ9t,12c are present in our diet, as result of heat treatment of vegetable oils. A nutritional study was carried out in order to obtain more precise information on the conversion of these two isomers into long chain polyunsaturated fatty acids (PUFA) by rat tissues. This in vivo study performed using rat fed with small quantities of mono trans linoleic acid isomers (0.6% of total energy) showed that 18:2 Δ9c,12t was converted into 20:4 Δ5c,8c,11c,14t while 18:2 Δ9t,12c was only slightly converted into 20:4 Δ5c,8c,11t,14c. Furthermore 18:2 Δ9t,12c was preferentially elongated into 20:2 Δ11t,14c. Each C20 metabolite of these mono trans 18:2 isomers was isolated as methyl ester by semi-preparative high-performance liquid chromatography (HPLC) followed by silver nitrate thin layer chromatography (AgNO3-TLC).The structure of the components was identified using partial hydrazine reduction, AgNO3-TLC of the resulting monoenes and gas-liquid chromatography coupled with mass spectrometry (GC-MS) of the 4,4-dimethyloxazoline (DMOX) derivatives. Fourier-transform-infrared spectroscopy (GC-FTIR) confirmed the frans geometry. Gas-liquid chromatography (GC) analyses showed that 18:2 Δ9c,12t and 18:2 Δ9t,12c were present in different tissue lipids (liver, heart, testes, brain and adipose tissue), and without any modification in the amount of 20:4n-6. 20:4 Δ5c, 8c,11c,14t was incorporated in different rat tissues except in brain. Furthermore, its incorporation followed that of its structural analogue, 20:3n-9 in liver phospholipid classes (phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine). Finally, an in vitro study carried out with rat liver microsomes showed that dietary trans 18:2 isomers could inhibit the Δ6- desaturation of 18:2n-6 to 18:3n-6 and the Δ5-desaturation of 20:3n-6 to 20:4n-6.
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| format | Article |
| id | doaj-art-7b4e8cbc0a324278b8543b04650bfb46 |
| institution | OA Journals |
| issn | 0017-3495 1988-4214 |
| language | English |
| publishDate | 1996-04-01 |
| publisher | Consejo Superior de Investigaciones Científicas |
| record_format | Article |
| series | Grasas y Aceites |
| spelling | doaj-art-7b4e8cbc0a324278b8543b04650bfb462025-08-20T02:23:26ZengConsejo Superior de Investigaciones CientíficasGrasas y Aceites0017-34951988-42141996-04-01471-210.3989/gya.1996.v47.i1-2.849833Effects of <i>trans</i> n-6 fatty acids on the fatty acid profile of tissues and liver microsomal desaturation in the ratOlivier Berdeaux0Jean Louis Sébédio1Jean Michel Chardigny2Jean Paul Blond3Thierry Mairot4Jean Michel Vatèles5Didier Poullain6Jean Pierre Nöel7NRA, Unite de Nutrition Lipidique, DijonNRA, Unite de Nutrition Lipidique, DijonNRA, Unite de Nutrition Lipidique, DijonUnite de Nutrition Cellulaire et Métabolique, Université de Bourgogne, DijonNRA, Unite de Nutrition Lipidique, DijonLaboratoire de Chimie Organique 1, Université Claude Bernard, LyonCEA-Saclay, Service des Molécules MarquéesCEA-Saclay, Service des Molécules Marquées 18:2Δ 9c,12t and 18:2 Δ9t,12c are present in our diet, as result of heat treatment of vegetable oils. A nutritional study was carried out in order to obtain more precise information on the conversion of these two isomers into long chain polyunsaturated fatty acids (PUFA) by rat tissues. This in vivo study performed using rat fed with small quantities of mono trans linoleic acid isomers (0.6% of total energy) showed that 18:2 Δ9c,12t was converted into 20:4 Δ5c,8c,11c,14t while 18:2 Δ9t,12c was only slightly converted into 20:4 Δ5c,8c,11t,14c. Furthermore 18:2 Δ9t,12c was preferentially elongated into 20:2 Δ11t,14c. Each C20 metabolite of these mono trans 18:2 isomers was isolated as methyl ester by semi-preparative high-performance liquid chromatography (HPLC) followed by silver nitrate thin layer chromatography (AgNO3-TLC).The structure of the components was identified using partial hydrazine reduction, AgNO3-TLC of the resulting monoenes and gas-liquid chromatography coupled with mass spectrometry (GC-MS) of the 4,4-dimethyloxazoline (DMOX) derivatives. Fourier-transform-infrared spectroscopy (GC-FTIR) confirmed the frans geometry. Gas-liquid chromatography (GC) analyses showed that 18:2 Δ9c,12t and 18:2 Δ9t,12c were present in different tissue lipids (liver, heart, testes, brain and adipose tissue), and without any modification in the amount of 20:4n-6. 20:4 Δ5c, 8c,11c,14t was incorporated in different rat tissues except in brain. Furthermore, its incorporation followed that of its structural analogue, 20:3n-9 in liver phospholipid classes (phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine). Finally, an in vitro study carried out with rat liver microsomes showed that dietary trans 18:2 isomers could inhibit the Δ6- desaturation of 18:2n-6 to 18:3n-6 and the Δ5-desaturation of 20:3n-6 to 20:4n-6. https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/849DesaturationDietLinoleic acidMetabolismMicrosomesRat |
| spellingShingle | Olivier Berdeaux Jean Louis Sébédio Jean Michel Chardigny Jean Paul Blond Thierry Mairot Jean Michel Vatèles Didier Poullain Jean Pierre Nöel Effects of <i>trans</i> n-6 fatty acids on the fatty acid profile of tissues and liver microsomal desaturation in the rat Grasas y Aceites Desaturation Diet Linoleic acid Metabolism Microsomes Rat |
| title | Effects of <i>trans</i> n-6 fatty acids on the fatty acid profile of tissues and liver microsomal desaturation in the rat |
| title_full | Effects of <i>trans</i> n-6 fatty acids on the fatty acid profile of tissues and liver microsomal desaturation in the rat |
| title_fullStr | Effects of <i>trans</i> n-6 fatty acids on the fatty acid profile of tissues and liver microsomal desaturation in the rat |
| title_full_unstemmed | Effects of <i>trans</i> n-6 fatty acids on the fatty acid profile of tissues and liver microsomal desaturation in the rat |
| title_short | Effects of <i>trans</i> n-6 fatty acids on the fatty acid profile of tissues and liver microsomal desaturation in the rat |
| title_sort | effects of i trans i n 6 fatty acids on the fatty acid profile of tissues and liver microsomal desaturation in the rat |
| topic | Desaturation Diet Linoleic acid Metabolism Microsomes Rat |
| url | https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/849 |
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