Long-Term Leukocyte Filtration Should Be Avoided during Extracorporeal Circulation
Filtration during extracorporeal circulation (ECC) not only removes but also activates leukocytes; therefore, long-term leukocyte filtration may cause adverse effects. In the present study, we tested this hypothesis by priming ECC with 300 mL of canine blood and examining filtration effects in 3 gro...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2013/612848 |
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| author | Jiali Tang Kaiyu Tao Jing Zhou Chongwei Zhang Lina Gong Nanfu Luo Lei Du |
| author_facet | Jiali Tang Kaiyu Tao Jing Zhou Chongwei Zhang Lina Gong Nanfu Luo Lei Du |
| author_sort | Jiali Tang |
| collection | DOAJ |
| description | Filtration during extracorporeal circulation (ECC) not only removes but also activates leukocytes; therefore, long-term leukocyte filtration may cause adverse effects. In the present study, we tested this hypothesis by priming ECC with 300 mL of canine blood and examining filtration effects in 3 groups (n=6 each) during 60 min ECC. In the control group (Group C) blood was filtrated with an arterial filter for 60 min; in long-term (Group L) and short-term (Group S) groups, blood was filtrated with a leukocyte filter for 60 and 5 min. We found that about 90% of leukocytes were removed after 5 min of filtration in both Groups L and S. Although leukocyte count continued to reduce, mean fluorescent intensities of CD11/CD18, free hemoglobin, and neutrophil elastase increased in Group L and were higher than those in Groups C and S at 60 min. Leukocyte rupture, cytoplasmic leakage, and circulating naked nuclei were also found in Group L. The data support our hypothesis that long-term filtration can induce inflammation and lead to leukocyte destruction. |
| format | Article |
| id | doaj-art-7b4a271682e84495a2e6240df2b75321 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-7b4a271682e84495a2e6240df2b753212025-02-03T07:25:30ZengWileyMediators of Inflammation0962-93511466-18612013-01-01201310.1155/2013/612848612848Long-Term Leukocyte Filtration Should Be Avoided during Extracorporeal CirculationJiali Tang0Kaiyu Tao1Jing Zhou2Chongwei Zhang3Lina Gong4Nanfu Luo5Lei Du6Department of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University, 37 Wainan Guoxuexiang, Chengdu, Sichuan 610041, ChinaDepartment of Thoracic Cardiovascular Surgery, Affiliated No. 2 Hospital, Zhejiang University, No. 88 Jiefang Road, Hangzhou, Zhejiang 310009, ChinaDepartment of Laboratory Medicine, West China Hospital, Sichuan University, 37 Wainan Guoxuexiang, Chengdu, Sichuan 610041, ChinaDepartment of Laboratory Medicine, West China Hospital, Sichuan University, 37 Wainan Guoxuexiang, Chengdu, Sichuan 610041, ChinaDepartment of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University, 37 Wainan Guoxuexiang, Chengdu, Sichuan 610041, ChinaDepartment of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University, 37 Wainan Guoxuexiang, Chengdu, Sichuan 610041, ChinaDepartment of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University, 37 Wainan Guoxuexiang, Chengdu, Sichuan 610041, ChinaFiltration during extracorporeal circulation (ECC) not only removes but also activates leukocytes; therefore, long-term leukocyte filtration may cause adverse effects. In the present study, we tested this hypothesis by priming ECC with 300 mL of canine blood and examining filtration effects in 3 groups (n=6 each) during 60 min ECC. In the control group (Group C) blood was filtrated with an arterial filter for 60 min; in long-term (Group L) and short-term (Group S) groups, blood was filtrated with a leukocyte filter for 60 and 5 min. We found that about 90% of leukocytes were removed after 5 min of filtration in both Groups L and S. Although leukocyte count continued to reduce, mean fluorescent intensities of CD11/CD18, free hemoglobin, and neutrophil elastase increased in Group L and were higher than those in Groups C and S at 60 min. Leukocyte rupture, cytoplasmic leakage, and circulating naked nuclei were also found in Group L. The data support our hypothesis that long-term filtration can induce inflammation and lead to leukocyte destruction.http://dx.doi.org/10.1155/2013/612848 |
| spellingShingle | Jiali Tang Kaiyu Tao Jing Zhou Chongwei Zhang Lina Gong Nanfu Luo Lei Du Long-Term Leukocyte Filtration Should Be Avoided during Extracorporeal Circulation Mediators of Inflammation |
| title | Long-Term Leukocyte Filtration Should Be Avoided during Extracorporeal Circulation |
| title_full | Long-Term Leukocyte Filtration Should Be Avoided during Extracorporeal Circulation |
| title_fullStr | Long-Term Leukocyte Filtration Should Be Avoided during Extracorporeal Circulation |
| title_full_unstemmed | Long-Term Leukocyte Filtration Should Be Avoided during Extracorporeal Circulation |
| title_short | Long-Term Leukocyte Filtration Should Be Avoided during Extracorporeal Circulation |
| title_sort | long term leukocyte filtration should be avoided during extracorporeal circulation |
| url | http://dx.doi.org/10.1155/2013/612848 |
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