Larvacide Activity of Tumih (Combretocarpus rotundatus (Miq.)Danser) Leaf Extracts against Aedes aegypti
This research aimed to measure the content of Combretocarpus Rotundatus (Miq.) Danser leaf extracts and to test its larvicidal activity against Aedes aegypti. The leaves were macerated and fractionated using methanol, n-hexane, ethyl acetate, and ethanol. The extract contents from the leaves extract...
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Universitas Gadjah Mada
2019-05-01
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| Series: | Jurnal Ilmu Kehutanan |
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| Online Access: | https://jurnal.ugm.ac.id/jikfkt/article/view/46208 |
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| author | Renhart Jemi Royda Dara Ertini Damanik Lies Indrayanti |
| author_facet | Renhart Jemi Royda Dara Ertini Damanik Lies Indrayanti |
| author_sort | Renhart Jemi |
| collection | DOAJ |
| description | This research aimed to measure the content of Combretocarpus Rotundatus (Miq.) Danser leaf extracts and to test its larvicidal activity against Aedes aegypti. The leaves were macerated and fractionated using methanol, n-hexane, ethyl acetate, and ethanol. The extract contents from the leaves extraction were metanol extract of 15%, n-hexane extract of 51%, ethyl acetate extract of 35% and ethanol extract of 85%. The larvicidal activity of extracts was tested with concentration of 0, 5, 10, 25, 50, 75, and 100 ppm. Phytochemicals test exhibited that the methanol extract of Combretocarpus rotundatus (Miq.) Danser leaves contained alkaloids, flavonoids, saponins, and tannins. Larvicidal test conducted on the extracts exhibited an effect on the mortality levels against Aedes aegypti larvae. The larvicidal activity of leaf extracts was optimum in the ethyl acetate extract at LC(50) = 24.54 ppm, methanol extract at LC(50) = 45.65 ppm, ethanol extract at LC(50) = 46.77 ppm, and n-hexane extract at LC(50 = 48.97 ppm. It was found that the ethyl acetate extract was the most active larvicide. FT-IR analysis showed existing functional groups of C-H alkanes and C=C aromatics. Those functional groups were assumed to be flavanoid, alkaloid, saponin, and tannin constituents. Results of LC-MS analysis indicated 7 bioactive compounds i.e.hexadecyl-ferulate, 21-o-methyl toosendanopentaol, 23-acetate alismaketone, dehydroxy-24-acetate alisol, prosapogenin 2, and stigmastan-3,6- dione. |
| format | Article |
| id | doaj-art-7b45529179c14f9598588318d30858a3 |
| institution | Kabale University |
| issn | 0126-4451 2477-3751 |
| language | English |
| publishDate | 2019-05-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Jurnal Ilmu Kehutanan |
| spelling | doaj-art-7b45529179c14f9598588318d30858a32025-01-23T03:53:03ZengUniversitas Gadjah MadaJurnal Ilmu Kehutanan0126-44512477-37512019-05-01131778610.22146/jik.4620824222Larvacide Activity of Tumih (Combretocarpus rotundatus (Miq.)Danser) Leaf Extracts against Aedes aegyptiRenhart Jemi0Royda Dara Ertini Damanik1Lies Indrayanti2Department of Forestry, Faculty of Agriculture, Universitas Palangka Raya, IndonesiaDepartment of Forestry, Faculty of Agriculture, Universitas Palangka Raya, IndonesiaDepartment of Forestry, Faculty of Agriculture, Universitas Palangka Raya, IndonesiaThis research aimed to measure the content of Combretocarpus Rotundatus (Miq.) Danser leaf extracts and to test its larvicidal activity against Aedes aegypti. The leaves were macerated and fractionated using methanol, n-hexane, ethyl acetate, and ethanol. The extract contents from the leaves extraction were metanol extract of 15%, n-hexane extract of 51%, ethyl acetate extract of 35% and ethanol extract of 85%. The larvicidal activity of extracts was tested with concentration of 0, 5, 10, 25, 50, 75, and 100 ppm. Phytochemicals test exhibited that the methanol extract of Combretocarpus rotundatus (Miq.) Danser leaves contained alkaloids, flavonoids, saponins, and tannins. Larvicidal test conducted on the extracts exhibited an effect on the mortality levels against Aedes aegypti larvae. The larvicidal activity of leaf extracts was optimum in the ethyl acetate extract at LC(50) = 24.54 ppm, methanol extract at LC(50) = 45.65 ppm, ethanol extract at LC(50) = 46.77 ppm, and n-hexane extract at LC(50 = 48.97 ppm. It was found that the ethyl acetate extract was the most active larvicide. FT-IR analysis showed existing functional groups of C-H alkanes and C=C aromatics. Those functional groups were assumed to be flavanoid, alkaloid, saponin, and tannin constituents. Results of LC-MS analysis indicated 7 bioactive compounds i.e.hexadecyl-ferulate, 21-o-methyl toosendanopentaol, 23-acetate alismaketone, dehydroxy-24-acetate alisol, prosapogenin 2, and stigmastan-3,6- dione.https://jurnal.ugm.ac.id/jikfkt/article/view/46208aedes aegyptimortalitlarvicideleaves extractlc(50) |
| spellingShingle | Renhart Jemi Royda Dara Ertini Damanik Lies Indrayanti Larvacide Activity of Tumih (Combretocarpus rotundatus (Miq.)Danser) Leaf Extracts against Aedes aegypti Jurnal Ilmu Kehutanan aedes aegypti mortalit larvicide leaves extract lc(50) |
| title | Larvacide Activity of Tumih (Combretocarpus rotundatus (Miq.)Danser) Leaf Extracts against Aedes aegypti |
| title_full | Larvacide Activity of Tumih (Combretocarpus rotundatus (Miq.)Danser) Leaf Extracts against Aedes aegypti |
| title_fullStr | Larvacide Activity of Tumih (Combretocarpus rotundatus (Miq.)Danser) Leaf Extracts against Aedes aegypti |
| title_full_unstemmed | Larvacide Activity of Tumih (Combretocarpus rotundatus (Miq.)Danser) Leaf Extracts against Aedes aegypti |
| title_short | Larvacide Activity of Tumih (Combretocarpus rotundatus (Miq.)Danser) Leaf Extracts against Aedes aegypti |
| title_sort | larvacide activity of tumih combretocarpus rotundatus miq danser leaf extracts against aedes aegypti |
| topic | aedes aegypti mortalit larvicide leaves extract lc(50) |
| url | https://jurnal.ugm.ac.id/jikfkt/article/view/46208 |
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