Bone‐targeted hybrid extracellular vesicles for alveolar bone regeneration
Abstract Prolonged tooth loss causes a blade‐like narrowing of the alveolar bone, severely impairing chewing function and aesthetics and complicating subsequent orthodontic or restorative treatments. Bone morphogenetic protein‐2 (BMP‐2) is widely used to induce osteogenesis; however, its lack of cel...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2025-05-01
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| Series: | Interdisciplinary Medicine |
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| Online Access: | https://doi.org/10.1002/INMD.20240126 |
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| author | Anqi Liu Gang Yang Yijie Zhao Jiajia Deng Jialiang Liu Kairun Zhang Li Mei Yan Liu Tingjiao Liu |
| author_facet | Anqi Liu Gang Yang Yijie Zhao Jiajia Deng Jialiang Liu Kairun Zhang Li Mei Yan Liu Tingjiao Liu |
| author_sort | Anqi Liu |
| collection | DOAJ |
| description | Abstract Prolonged tooth loss causes a blade‐like narrowing of the alveolar bone, severely impairing chewing function and aesthetics and complicating subsequent orthodontic or restorative treatments. Bone morphogenetic protein‐2 (BMP‐2) is widely used to induce osteogenesis; however, its lack of cellular targeting in complex microenvironments often results in significant side effects. Developing a safe, stable, and osteoblast‐targeted drug delivery system is crucial for precise bone regeneration. Nanoparticles, as ideal drug delivery vehicles, offer highly controllable cellular targeting. This study introduces an innovative approach using DNA nanostructure‐modified BMP‐2‐loaded hybrid extracellular vesicles (EVs) formed by fusing liposomes and EVs. Screening identified 180 nm as the optimal particle size for EVs fusion efficiency. The system achieved osteoblast‐specific targeting by attaching the DNA aptamer 19S to the hybrid EVs membrane. The hybrid EVs were further combined with a hydrogel sustained‐release system, creating a drug delivery platform that effectively repaired alveolar bone defects. This approach demonstrated significant potential for advancing bone tissue repair and regeneration. |
| format | Article |
| id | doaj-art-7b3a991863de48519985ded786bee3e7 |
| institution | Kabale University |
| issn | 2832-6245 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Interdisciplinary Medicine |
| spelling | doaj-art-7b3a991863de48519985ded786bee3e72025-08-20T03:26:39ZengWiley-VCHInterdisciplinary Medicine2832-62452025-05-0133n/an/a10.1002/INMD.20240126Bone‐targeted hybrid extracellular vesicles for alveolar bone regenerationAnqi Liu0Gang Yang1Yijie Zhao2Jiajia Deng3Jialiang Liu4Kairun Zhang5Li Mei6Yan Liu7Tingjiao Liu8Department of Orthodontics School of Medicine Shanghai Ninth People's Hospital College of Stomatology Shanghai Jiao Tong University Shanghai ChinaDepartment of Multidisciplinary Consultant Center Shanghai Stomatological Hospital & School of Stomatology Shanghai ChinaShanghai Key Laboratory of Craniomaxillofacial Development and Diseases Fudan University Shanghai ChinaShanghai Key Laboratory of Craniomaxillofacial Development and Diseases Fudan University Shanghai ChinaShanghai Key Laboratory of Craniomaxillofacial Development and Diseases Fudan University Shanghai ChinaShanghai Key Laboratory of Craniomaxillofacial Development and Diseases Fudan University Shanghai ChinaDepartment of Oral Sciences Faculty of Dentistry University of Otago Dunedin New ZealandCentral Laboratory Peking University School and Hospital for Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices Beijing ChinaShanghai Key Laboratory of Craniomaxillofacial Development and Diseases Fudan University Shanghai ChinaAbstract Prolonged tooth loss causes a blade‐like narrowing of the alveolar bone, severely impairing chewing function and aesthetics and complicating subsequent orthodontic or restorative treatments. Bone morphogenetic protein‐2 (BMP‐2) is widely used to induce osteogenesis; however, its lack of cellular targeting in complex microenvironments often results in significant side effects. Developing a safe, stable, and osteoblast‐targeted drug delivery system is crucial for precise bone regeneration. Nanoparticles, as ideal drug delivery vehicles, offer highly controllable cellular targeting. This study introduces an innovative approach using DNA nanostructure‐modified BMP‐2‐loaded hybrid extracellular vesicles (EVs) formed by fusing liposomes and EVs. Screening identified 180 nm as the optimal particle size for EVs fusion efficiency. The system achieved osteoblast‐specific targeting by attaching the DNA aptamer 19S to the hybrid EVs membrane. The hybrid EVs were further combined with a hydrogel sustained‐release system, creating a drug delivery platform that effectively repaired alveolar bone defects. This approach demonstrated significant potential for advancing bone tissue repair and regeneration.https://doi.org/10.1002/INMD.20240126alveolar bone defectbone targetingDNA nanostructureshybrid EVsliposomes |
| spellingShingle | Anqi Liu Gang Yang Yijie Zhao Jiajia Deng Jialiang Liu Kairun Zhang Li Mei Yan Liu Tingjiao Liu Bone‐targeted hybrid extracellular vesicles for alveolar bone regeneration Interdisciplinary Medicine alveolar bone defect bone targeting DNA nanostructures hybrid EVs liposomes |
| title | Bone‐targeted hybrid extracellular vesicles for alveolar bone regeneration |
| title_full | Bone‐targeted hybrid extracellular vesicles for alveolar bone regeneration |
| title_fullStr | Bone‐targeted hybrid extracellular vesicles for alveolar bone regeneration |
| title_full_unstemmed | Bone‐targeted hybrid extracellular vesicles for alveolar bone regeneration |
| title_short | Bone‐targeted hybrid extracellular vesicles for alveolar bone regeneration |
| title_sort | bone targeted hybrid extracellular vesicles for alveolar bone regeneration |
| topic | alveolar bone defect bone targeting DNA nanostructures hybrid EVs liposomes |
| url | https://doi.org/10.1002/INMD.20240126 |
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