Potential Clinical Application of Analysis of Bisphenols in Pericardial Fluid from Patients with Coronary Artery Disease with the Use of Liquid Chromatography Combined with Fluorescence Detection and Triple Quadrupole Mass Spectrometry
Bisphenols may negatively impact human health. In this study, we propose the use of HPLC–FLD for the simultaneous determination of bisphenols in pericardial fluid samples collected from patients with coronary artery disease undergoing coronary artery bypass surgery. For sample preparation, a fast, s...
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2025-01-01
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| author | Tomasz Tuzimski Szymon Szubartowski Janusz Stążka Kamil Baczewski Daria Janiszewska Viorica Railean Bogusław Buszewski Małgorzata Szultka-Młyńska |
| author_facet | Tomasz Tuzimski Szymon Szubartowski Janusz Stążka Kamil Baczewski Daria Janiszewska Viorica Railean Bogusław Buszewski Małgorzata Szultka-Młyńska |
| author_sort | Tomasz Tuzimski |
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| description | Bisphenols may negatively impact human health. In this study, we propose the use of HPLC–FLD for the simultaneous determination of bisphenols in pericardial fluid samples collected from patients with coronary artery disease undergoing coronary artery bypass surgery. For sample preparation, a fast, simple, and ”green” DLLME method was used, achieving mean recovery values in the range of 62%–98% with relative standard deviations between 2% and 6% for all analytes. Quantitative analysis of bisphenols in the samples was then performed by LC–MS/MS on a triple quadrupole (QqQ) mass spectrometer and electrospray ionization (ESI-/ESI+) was applied in the negative and positive ion modes, respectively. The LODs and LOQs ranged from 0.04 ng/mL to 0.37 ng/mL and 0.12 ng/mL to 1.11 ng/mL, respectively. Pericardial fluid was collected from patients with coronary artery disease during coronary artery bypass surgery. Bisphenol residues were identified and quantified in samples from 19 patients. The procedure was successfully applied to the biomonitoring of free forms of 14 bisphenols in pericardial fluid. After statistical examination of the relationships between the selected variables, a strongly positive correlation was found between creatinine kinase and troponin I, as well as the number of venous anastomoses, circulation time, and clamp cap time. |
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| institution | Kabale University |
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| publishDate | 2025-01-01 |
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| spelling | doaj-art-7b2b551a7170458da6dccf52849d543d2025-01-10T13:19:00ZengMDPI AGMolecules1420-30492025-01-0130114010.3390/molecules30010140Potential Clinical Application of Analysis of Bisphenols in Pericardial Fluid from Patients with Coronary Artery Disease with the Use of Liquid Chromatography Combined with Fluorescence Detection and Triple Quadrupole Mass SpectrometryTomasz Tuzimski0Szymon Szubartowski1Janusz Stążka2Kamil Baczewski3Daria Janiszewska4Viorica Railean5Bogusław Buszewski6Małgorzata Szultka-Młyńska7Department of Physical Chemistry, Faculty of Pharmacy, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, PolandDepartment of Physical Chemistry, Faculty of Pharmacy, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, PolandDepartment of Cardiac Surgery, Medical University of Lublin, Jaczewskiego 8 (USK Nr 4), 20-093 Lublin, PolandDepartment of Cardiac Surgery, Medical University of Lublin, Jaczewskiego 8 (USK Nr 4), 20-093 Lublin, PolandDepartment of Environmental Chemistry and Bioanalytics, Gagarina 7, Faculty of Chemistry, Nicolaus Copernicus University, 87-100 Torun, PolandDepartment of Infectious, Invasive Diseases and Veterinary Administration, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, Gagarina 7, 87-100 Torun, PolandDepartment of Environmental Chemistry and Bioanalytics, Gagarina 7, Faculty of Chemistry, Nicolaus Copernicus University, 87-100 Torun, PolandDepartment of Environmental Chemistry and Bioanalytics, Gagarina 7, Faculty of Chemistry, Nicolaus Copernicus University, 87-100 Torun, PolandBisphenols may negatively impact human health. In this study, we propose the use of HPLC–FLD for the simultaneous determination of bisphenols in pericardial fluid samples collected from patients with coronary artery disease undergoing coronary artery bypass surgery. For sample preparation, a fast, simple, and ”green” DLLME method was used, achieving mean recovery values in the range of 62%–98% with relative standard deviations between 2% and 6% for all analytes. Quantitative analysis of bisphenols in the samples was then performed by LC–MS/MS on a triple quadrupole (QqQ) mass spectrometer and electrospray ionization (ESI-/ESI+) was applied in the negative and positive ion modes, respectively. The LODs and LOQs ranged from 0.04 ng/mL to 0.37 ng/mL and 0.12 ng/mL to 1.11 ng/mL, respectively. Pericardial fluid was collected from patients with coronary artery disease during coronary artery bypass surgery. Bisphenol residues were identified and quantified in samples from 19 patients. The procedure was successfully applied to the biomonitoring of free forms of 14 bisphenols in pericardial fluid. After statistical examination of the relationships between the selected variables, a strongly positive correlation was found between creatinine kinase and troponin I, as well as the number of venous anastomoses, circulation time, and clamp cap time.https://www.mdpi.com/1420-3049/30/1/140dispersive liquid–liquid microextraction (DLLME)LC–ESI–QqQpericardial fluidbisphenolbisphenol A diglicydyl ether (BADGE) with analogs |
| spellingShingle | Tomasz Tuzimski Szymon Szubartowski Janusz Stążka Kamil Baczewski Daria Janiszewska Viorica Railean Bogusław Buszewski Małgorzata Szultka-Młyńska Potential Clinical Application of Analysis of Bisphenols in Pericardial Fluid from Patients with Coronary Artery Disease with the Use of Liquid Chromatography Combined with Fluorescence Detection and Triple Quadrupole Mass Spectrometry Molecules dispersive liquid–liquid microextraction (DLLME) LC–ESI–QqQ pericardial fluid bisphenol bisphenol A diglicydyl ether (BADGE) with analogs |
| title | Potential Clinical Application of Analysis of Bisphenols in Pericardial Fluid from Patients with Coronary Artery Disease with the Use of Liquid Chromatography Combined with Fluorescence Detection and Triple Quadrupole Mass Spectrometry |
| title_full | Potential Clinical Application of Analysis of Bisphenols in Pericardial Fluid from Patients with Coronary Artery Disease with the Use of Liquid Chromatography Combined with Fluorescence Detection and Triple Quadrupole Mass Spectrometry |
| title_fullStr | Potential Clinical Application of Analysis of Bisphenols in Pericardial Fluid from Patients with Coronary Artery Disease with the Use of Liquid Chromatography Combined with Fluorescence Detection and Triple Quadrupole Mass Spectrometry |
| title_full_unstemmed | Potential Clinical Application of Analysis of Bisphenols in Pericardial Fluid from Patients with Coronary Artery Disease with the Use of Liquid Chromatography Combined with Fluorescence Detection and Triple Quadrupole Mass Spectrometry |
| title_short | Potential Clinical Application of Analysis of Bisphenols in Pericardial Fluid from Patients with Coronary Artery Disease with the Use of Liquid Chromatography Combined with Fluorescence Detection and Triple Quadrupole Mass Spectrometry |
| title_sort | potential clinical application of analysis of bisphenols in pericardial fluid from patients with coronary artery disease with the use of liquid chromatography combined with fluorescence detection and triple quadrupole mass spectrometry |
| topic | dispersive liquid–liquid microextraction (DLLME) LC–ESI–QqQ pericardial fluid bisphenol bisphenol A diglicydyl ether (BADGE) with analogs |
| url | https://www.mdpi.com/1420-3049/30/1/140 |
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