High Blood Pressure Effects on the Blood to Cerebrospinal Fluid Barrier and Cerebrospinal Fluid Protein Composition: A Two-Dimensional Electrophoresis Study in Spontaneously Hypertensive Rats
The aim of the present work is to analyze the cerebrospinal fluid proteomic profile, trying to find possible biomarkers of the effects of hypertension of the blood to CSF barrier disruption in the brain and their participation in the cholesterol and β-amyloid metabolism and inflammatory processes. C...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Hypertension |
| Online Access: | http://dx.doi.org/10.1155/2013/164653 |
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| author | Ibrahim González-Marrero Leandro Castañeyra-Ruiz Juan M. González-Toledo Agustín Castañeyra-Ruiz Hector de Paz-Carmona Rafael Castro Juan R. Hernandez-Fernaud Agustín Castañeyra-Perdomo Emilia M. Carmona-Calero |
| author_facet | Ibrahim González-Marrero Leandro Castañeyra-Ruiz Juan M. González-Toledo Agustín Castañeyra-Ruiz Hector de Paz-Carmona Rafael Castro Juan R. Hernandez-Fernaud Agustín Castañeyra-Perdomo Emilia M. Carmona-Calero |
| author_sort | Ibrahim González-Marrero |
| collection | DOAJ |
| description | The aim of the present work is to analyze the cerebrospinal fluid proteomic profile, trying to find possible biomarkers of the effects of hypertension of the blood to CSF barrier disruption in the brain and their participation in the cholesterol and β-amyloid metabolism and inflammatory processes. Cerebrospinal fluid (CSF) is a system linked to the brain and its composition can be altered not only by encephalic disorder, but also by systemic diseases such as arterial hypertension, which produces alterations in the choroid plexus and cerebrospinal fluid protein composition. 2D gel electrophoresis in cerebrospinal fluid extracted from the cistern magna before sacrifice of hypertensive and control rats was performed. The results showed different proteomic profiles between SHR and WKY, that α-1-antitrypsin, apolipoprotein A1, albumin, immunoglobulin G, vitamin D binding protein, haptoglobin and α-1-macroglobulin were found to be up-regulated in SHR, and apolipoprotein E, transthyretin, α-2-HS-glycoprotein, transferrin, α-1β-glycoprotein, kininogen and carbonic anhidrase II were down-regulated in SHR. The conclusion made here is that hypertension in SHR produces important variations in cerebrospinal fluid proteins that could be due to a choroid plexus dysfunction and this fact supports the close connection between hypertension and blood to cerebrospinal fluid barrier disruption. |
| format | Article |
| id | doaj-art-7b27671ba11146e99026b59166916219 |
| institution | Kabale University |
| issn | 2090-0384 2090-0392 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Hypertension |
| spelling | doaj-art-7b27671ba11146e99026b591669162192025-02-03T05:53:20ZengWileyInternational Journal of Hypertension2090-03842090-03922013-01-01201310.1155/2013/164653164653High Blood Pressure Effects on the Blood to Cerebrospinal Fluid Barrier and Cerebrospinal Fluid Protein Composition: A Two-Dimensional Electrophoresis Study in Spontaneously Hypertensive RatsIbrahim González-Marrero0Leandro Castañeyra-Ruiz1Juan M. González-Toledo2Agustín Castañeyra-Ruiz3Hector de Paz-Carmona4Rafael Castro5Juan R. Hernandez-Fernaud6Agustín Castañeyra-Perdomo7Emilia M. Carmona-Calero8Departamento de Anatomía, Facultad de Medicina, Universidad de La Laguna, Ofra s/n, 38071 La Laguna, Tenerife, SpainDepartamento de Anatomía, Facultad de Medicina, Universidad de La Laguna, Ofra s/n, 38071 La Laguna, Tenerife, SpainDepartamento de Anatomía, Facultad de Medicina, Universidad de La Laguna, Ofra s/n, 38071 La Laguna, Tenerife, SpainInstituto de Investigación y Ciencias de Puerto del Rosario, c/Tenerife n 35, 35600 Puerto del Rosario, Fuerteventura, SpainInstituto de Investigación y Ciencias de Puerto del Rosario, c/Tenerife n 35, 35600 Puerto del Rosario, Fuerteventura, SpainDepartamento de Fisiología, Facultad de Medicina, Universidad de La Laguna, Ofra s/n, 38071 La Laguna, Tenerife, SpainInstituto de Investigación y Ciencias de Puerto del Rosario, c/Tenerife n 35, 35600 Puerto del Rosario, Fuerteventura, SpainDepartamento de Anatomía, Facultad de Medicina, Universidad de La Laguna, Ofra s/n, 38071 La Laguna, Tenerife, SpainDepartamento de Anatomía, Facultad de Medicina, Universidad de La Laguna, Ofra s/n, 38071 La Laguna, Tenerife, SpainThe aim of the present work is to analyze the cerebrospinal fluid proteomic profile, trying to find possible biomarkers of the effects of hypertension of the blood to CSF barrier disruption in the brain and their participation in the cholesterol and β-amyloid metabolism and inflammatory processes. Cerebrospinal fluid (CSF) is a system linked to the brain and its composition can be altered not only by encephalic disorder, but also by systemic diseases such as arterial hypertension, which produces alterations in the choroid plexus and cerebrospinal fluid protein composition. 2D gel electrophoresis in cerebrospinal fluid extracted from the cistern magna before sacrifice of hypertensive and control rats was performed. The results showed different proteomic profiles between SHR and WKY, that α-1-antitrypsin, apolipoprotein A1, albumin, immunoglobulin G, vitamin D binding protein, haptoglobin and α-1-macroglobulin were found to be up-regulated in SHR, and apolipoprotein E, transthyretin, α-2-HS-glycoprotein, transferrin, α-1β-glycoprotein, kininogen and carbonic anhidrase II were down-regulated in SHR. The conclusion made here is that hypertension in SHR produces important variations in cerebrospinal fluid proteins that could be due to a choroid plexus dysfunction and this fact supports the close connection between hypertension and blood to cerebrospinal fluid barrier disruption.http://dx.doi.org/10.1155/2013/164653 |
| spellingShingle | Ibrahim González-Marrero Leandro Castañeyra-Ruiz Juan M. González-Toledo Agustín Castañeyra-Ruiz Hector de Paz-Carmona Rafael Castro Juan R. Hernandez-Fernaud Agustín Castañeyra-Perdomo Emilia M. Carmona-Calero High Blood Pressure Effects on the Blood to Cerebrospinal Fluid Barrier and Cerebrospinal Fluid Protein Composition: A Two-Dimensional Electrophoresis Study in Spontaneously Hypertensive Rats International Journal of Hypertension |
| title | High Blood Pressure Effects on the Blood to Cerebrospinal Fluid Barrier and Cerebrospinal Fluid Protein Composition: A Two-Dimensional Electrophoresis Study in Spontaneously Hypertensive Rats |
| title_full | High Blood Pressure Effects on the Blood to Cerebrospinal Fluid Barrier and Cerebrospinal Fluid Protein Composition: A Two-Dimensional Electrophoresis Study in Spontaneously Hypertensive Rats |
| title_fullStr | High Blood Pressure Effects on the Blood to Cerebrospinal Fluid Barrier and Cerebrospinal Fluid Protein Composition: A Two-Dimensional Electrophoresis Study in Spontaneously Hypertensive Rats |
| title_full_unstemmed | High Blood Pressure Effects on the Blood to Cerebrospinal Fluid Barrier and Cerebrospinal Fluid Protein Composition: A Two-Dimensional Electrophoresis Study in Spontaneously Hypertensive Rats |
| title_short | High Blood Pressure Effects on the Blood to Cerebrospinal Fluid Barrier and Cerebrospinal Fluid Protein Composition: A Two-Dimensional Electrophoresis Study in Spontaneously Hypertensive Rats |
| title_sort | high blood pressure effects on the blood to cerebrospinal fluid barrier and cerebrospinal fluid protein composition a two dimensional electrophoresis study in spontaneously hypertensive rats |
| url | http://dx.doi.org/10.1155/2013/164653 |
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