Multi modal optical coherence tomography flowmetry of organ on chip devices
Abstract Organ-on-chip (OoC) systems are microfluidic devices for maintaining live tissue under physiologically relevant (flow) conditions. Imaging of structure and flow is important for the characterization of OoC device design and visualizing tissue/fluid interaction. Here, we present 3D tissue an...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-10089-9 |
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| author | Devrim Tugberk Konstantine Cheishvili Peter Speets William Quirós-Solano Anish Ballal Nikolas Gaio Jeroen Kalkman |
| author_facet | Devrim Tugberk Konstantine Cheishvili Peter Speets William Quirós-Solano Anish Ballal Nikolas Gaio Jeroen Kalkman |
| author_sort | Devrim Tugberk |
| collection | DOAJ |
| description | Abstract Organ-on-chip (OoC) systems are microfluidic devices for maintaining live tissue under physiologically relevant (flow) conditions. Imaging of structure and flow is important for the characterization of OoC device design and visualizing tissue/fluid interaction. Here, we present 3D tissue and flow imaging in an OoC device with multi-modal optical coherence tomography (OCT) using a combination of OCT structural imaging and flow imaging with Doppler OCT, number fluctuation dynamic light scattering OCT, and particle image velocimetry OCT. We demonstrate the feasibility of combined imaging of OoC tissue culture morphology and high flow velocities. We also measure low velocities in the OoC tissue well showing good agreement with computational fluid dynamics simulations. Our results open up the way for studying the effect of flow on living tissue in OoC devices. |
| format | Article |
| id | doaj-art-7b1bee5ecb444846a1567acd86a63f41 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-7b1bee5ecb444846a1567acd86a63f412025-08-20T03:46:03ZengNature PortfolioScientific Reports2045-23222025-07-011511910.1038/s41598-025-10089-9Multi modal optical coherence tomography flowmetry of organ on chip devicesDevrim Tugberk0Konstantine Cheishvili1Peter Speets2William Quirós-Solano3Anish Ballal4Nikolas Gaio5Jeroen Kalkman6Department of Imaging Physics, Delft University of TechnologyDepartment of Imaging Physics, Delft University of TechnologyDepartment of Imaging Physics, Delft University of TechnologyBIOND Solutions B.V.BIOND Solutions B.V.BIOND Solutions B.V.Department of Imaging Physics, Delft University of TechnologyAbstract Organ-on-chip (OoC) systems are microfluidic devices for maintaining live tissue under physiologically relevant (flow) conditions. Imaging of structure and flow is important for the characterization of OoC device design and visualizing tissue/fluid interaction. Here, we present 3D tissue and flow imaging in an OoC device with multi-modal optical coherence tomography (OCT) using a combination of OCT structural imaging and flow imaging with Doppler OCT, number fluctuation dynamic light scattering OCT, and particle image velocimetry OCT. We demonstrate the feasibility of combined imaging of OoC tissue culture morphology and high flow velocities. We also measure low velocities in the OoC tissue well showing good agreement with computational fluid dynamics simulations. Our results open up the way for studying the effect of flow on living tissue in OoC devices.https://doi.org/10.1038/s41598-025-10089-9 |
| spellingShingle | Devrim Tugberk Konstantine Cheishvili Peter Speets William Quirós-Solano Anish Ballal Nikolas Gaio Jeroen Kalkman Multi modal optical coherence tomography flowmetry of organ on chip devices Scientific Reports |
| title | Multi modal optical coherence tomography flowmetry of organ on chip devices |
| title_full | Multi modal optical coherence tomography flowmetry of organ on chip devices |
| title_fullStr | Multi modal optical coherence tomography flowmetry of organ on chip devices |
| title_full_unstemmed | Multi modal optical coherence tomography flowmetry of organ on chip devices |
| title_short | Multi modal optical coherence tomography flowmetry of organ on chip devices |
| title_sort | multi modal optical coherence tomography flowmetry of organ on chip devices |
| url | https://doi.org/10.1038/s41598-025-10089-9 |
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