Expansion of metabolically labelled endocytic organelles and cytoskeletal cell structures in Giardia lamblia using optimised U-ExM protocols
Understanding cellular ultrastructure is tightly bound to microscopic resolution and the ability to identify individual components at that resolution. Expansion microscopy has revolutionised this topic. Here we present and compare two protocols of ultrastructure expansion microscopy that allow for 4...
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| Format: | Article |
| Language: | English |
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Shared Science Publishers OG
2024-06-01
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| Series: | Microbial Cell |
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| Online Access: | http://microbialcell.com/researcharticles/2024a-jetishi-microbial-cell/ |
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| author | Clirim Jetishi Erina A Balmer Bianca M Berger Carmen Faso Torsten Ochsenreiter |
| author_facet | Clirim Jetishi Erina A Balmer Bianca M Berger Carmen Faso Torsten Ochsenreiter |
| author_sort | Clirim Jetishi |
| collection | DOAJ |
| description | Understanding cellular ultrastructure is tightly bound to microscopic resolution and the ability to identify individual components at that resolution. Expansion microscopy has revolutionised this topic. Here we present and compare two protocols of ultrastructure expansion microscopy that allow for 4.5-fold mostly isotropic expansion and the use of antibodies, metabolic labelling, and DNA stains to demarcate individual regions such as the endoplasmic reticulum, the nuclei, the peripheral endocytic compartments as well as the ventral disc and the cytoskeleton in Giardia lamblia. We present an optimised, shortened, and modular protocol that can be swiftly adjusted to the investigators needs in this important protozoan model organism. |
| format | Article |
| id | doaj-art-7b19c96b5d8e42dd8ecfc2bf394741de |
| institution | DOAJ |
| issn | 2311-2638 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Shared Science Publishers OG |
| record_format | Article |
| series | Microbial Cell |
| spelling | doaj-art-7b19c96b5d8e42dd8ecfc2bf394741de2025-08-20T02:52:38ZengShared Science Publishers OGMicrobial Cell2311-26382024-06-011119820610.15698/mic2024.06.825Expansion of metabolically labelled endocytic organelles and cytoskeletal cell structures in Giardia lamblia using optimised U-ExM protocolsClirim Jetishi0https://orcid.org/0000-0002-5632-7075Erina A Balmer1https://orcid.org/0000-0003-4815-2065Bianca M Berger2https://orcid.org/0000-0003-1009-4293Carmen Faso3https://orcid.org/0000-0002-1831-9365Torsten Ochsenreiter4https://orcid.org/0000-0002-8846-8526Institute of Cell Biology, University of Bern, Bern, SwitzerlandInstitute of Cell Biology, University of Bern, Bern, SwitzerlandInstitute of Cell Biology, University of Bern, Bern, SwitzerlandInstitute of Cell Biology, University of Bern, Bern, SwitzerlandInstitute of Cell Biology, University of Bern, Bern, SwitzerlandUnderstanding cellular ultrastructure is tightly bound to microscopic resolution and the ability to identify individual components at that resolution. Expansion microscopy has revolutionised this topic. Here we present and compare two protocols of ultrastructure expansion microscopy that allow for 4.5-fold mostly isotropic expansion and the use of antibodies, metabolic labelling, and DNA stains to demarcate individual regions such as the endoplasmic reticulum, the nuclei, the peripheral endocytic compartments as well as the ventral disc and the cytoskeleton in Giardia lamblia. We present an optimised, shortened, and modular protocol that can be swiftly adjusted to the investigators needs in this important protozoan model organism.http://microbialcell.com/researcharticles/2024a-jetishi-microbial-cell/giardia lambliaexpansion microscopysubcellular compartmentmetabolic labellingendocytosiscytoskeletonendoplasmic reticulum |
| spellingShingle | Clirim Jetishi Erina A Balmer Bianca M Berger Carmen Faso Torsten Ochsenreiter Expansion of metabolically labelled endocytic organelles and cytoskeletal cell structures in Giardia lamblia using optimised U-ExM protocols Microbial Cell giardia lamblia expansion microscopy subcellular compartment metabolic labelling endocytosis cytoskeleton endoplasmic reticulum |
| title | Expansion of metabolically labelled endocytic organelles and cytoskeletal cell structures in Giardia lamblia using optimised U-ExM protocols |
| title_full | Expansion of metabolically labelled endocytic organelles and cytoskeletal cell structures in Giardia lamblia using optimised U-ExM protocols |
| title_fullStr | Expansion of metabolically labelled endocytic organelles and cytoskeletal cell structures in Giardia lamblia using optimised U-ExM protocols |
| title_full_unstemmed | Expansion of metabolically labelled endocytic organelles and cytoskeletal cell structures in Giardia lamblia using optimised U-ExM protocols |
| title_short | Expansion of metabolically labelled endocytic organelles and cytoskeletal cell structures in Giardia lamblia using optimised U-ExM protocols |
| title_sort | expansion of metabolically labelled endocytic organelles and cytoskeletal cell structures in giardia lamblia using optimised u exm protocols |
| topic | giardia lamblia expansion microscopy subcellular compartment metabolic labelling endocytosis cytoskeleton endoplasmic reticulum |
| url | http://microbialcell.com/researcharticles/2024a-jetishi-microbial-cell/ |
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