Ultrasound-assisted extraction of phenolic compounds from ear mushrooms (Auricularia auricula-judae): Assessing composition and antioxidant activity during fruiting body development
Auricularia auricula-judae (ear mushroom) exhibits significant biological and pharmacological properties, particularly as an antioxidant due to its phenolic compounds. This study introduces a novel ultrasound-assisted extraction technique to quantify phenolic compounds and assess antioxidant activit...
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| Format: | Article |
| Language: | English |
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AIMS Press
2024-12-01
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| Series: | AIMS Agriculture and Food |
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| Online Access: | https://www.aimspress.com/article/doi/10.3934/agrfood.2024059 |
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| author | Albert Nugraha Asadin Briliantama M Umar Harun Li Sing-Chung Chin Xuan Tan Vuanghao Lim Amir Husni Widiastuti Setyaningsih |
| author_facet | Albert Nugraha Asadin Briliantama M Umar Harun Li Sing-Chung Chin Xuan Tan Vuanghao Lim Amir Husni Widiastuti Setyaningsih |
| author_sort | Albert Nugraha |
| collection | DOAJ |
| description | Auricularia auricula-judae (ear mushroom) exhibits significant biological and pharmacological properties, particularly as an antioxidant due to its phenolic compounds. This study introduces a novel ultrasound-assisted extraction technique to quantify phenolic compounds and assess antioxidant activity in ear mushrooms. Key extraction factors, including solvent-to-sample ratio (10:1, 20:1, 30:1 mL/g), pulse duty cycle (0.2, 0.5, 0.8 s-1), and temperature (10, 35, 60 ℃), were optimized using a Box–Behnken design and response surface methodology (RSM). Methanol was identified as the most effective solvent, yielding the highest total phenolic content (TPC) and antioxidant activity. The optimal conditions for TPC and 2, 2-diphenyl-1-picryl hydrazyl (DPPH) inhibition were determined to be 1 g of sample with 18 mL of methanol at 59 ℃ and a pulse duty cycle of 0.7 s-1. This method achieved a recovery rate of 94.85% for TPC and 92.71% for antioxidant inhibition and was validated with high precision (CV < 5%). Application of this method during fruiting body development (7–19 d) revealed that the optimal harvest time for maximum TPC and antioxidant activity was 8 d fruiting age. |
| format | Article |
| id | doaj-art-7b157d7afc134d969320367d966d0eec |
| institution | Kabale University |
| issn | 2471-2086 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | AIMS Press |
| record_format | Article |
| series | AIMS Agriculture and Food |
| spelling | doaj-art-7b157d7afc134d969320367d966d0eec2025-01-24T01:24:30ZengAIMS PressAIMS Agriculture and Food2471-20862024-12-01941134115010.3934/agrfood.2024059Ultrasound-assisted extraction of phenolic compounds from ear mushrooms (Auricularia auricula-judae): Assessing composition and antioxidant activity during fruiting body developmentAlbert Nugraha0Asadin Briliantama1M Umar Harun2Li Sing-Chung3Chin Xuan Tan4Vuanghao Lim5Amir Husni6Widiastuti Setyaningsih7Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jalan Flora, Depok, Sleman, Yogyakarta 55281, IndonesiaDepartment of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jalan Flora, Depok, Sleman, Yogyakarta 55281, IndonesiaDepartment of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jalan Flora, Depok, Sleman, Yogyakarta 55281, IndonesiaSchool of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei City 11031, TaiwanDepartment of Allied Health Sciences, Faculty of Science, Universiti Tunku Abdul Rahman, Jalan Universiti, Kampar, MalaysiaDepartment of Toxicology, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam 13200, Kepala Batas, Penang, MalaysiaFaculty of Agriculture Universitas Gadjah Mada Jalan Flora, Depok, Sleman, Yogyakarta 55281, IndonesiaDepartment of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jalan Flora, Depok, Sleman, Yogyakarta 55281, IndonesiaAuricularia auricula-judae (ear mushroom) exhibits significant biological and pharmacological properties, particularly as an antioxidant due to its phenolic compounds. This study introduces a novel ultrasound-assisted extraction technique to quantify phenolic compounds and assess antioxidant activity in ear mushrooms. Key extraction factors, including solvent-to-sample ratio (10:1, 20:1, 30:1 mL/g), pulse duty cycle (0.2, 0.5, 0.8 s-1), and temperature (10, 35, 60 ℃), were optimized using a Box–Behnken design and response surface methodology (RSM). Methanol was identified as the most effective solvent, yielding the highest total phenolic content (TPC) and antioxidant activity. The optimal conditions for TPC and 2, 2-diphenyl-1-picryl hydrazyl (DPPH) inhibition were determined to be 1 g of sample with 18 mL of methanol at 59 ℃ and a pulse duty cycle of 0.7 s-1. This method achieved a recovery rate of 94.85% for TPC and 92.71% for antioxidant inhibition and was validated with high precision (CV < 5%). Application of this method during fruiting body development (7–19 d) revealed that the optimal harvest time for maximum TPC and antioxidant activity was 8 d fruiting age.https://www.aimspress.com/article/doi/10.3934/agrfood.2024059box-behnken designmethod developmenttpcantioxidantvalidation |
| spellingShingle | Albert Nugraha Asadin Briliantama M Umar Harun Li Sing-Chung Chin Xuan Tan Vuanghao Lim Amir Husni Widiastuti Setyaningsih Ultrasound-assisted extraction of phenolic compounds from ear mushrooms (Auricularia auricula-judae): Assessing composition and antioxidant activity during fruiting body development AIMS Agriculture and Food box-behnken design method development tpc antioxidant validation |
| title | Ultrasound-assisted extraction of phenolic compounds from ear mushrooms (Auricularia auricula-judae): Assessing composition and antioxidant activity during fruiting body development |
| title_full | Ultrasound-assisted extraction of phenolic compounds from ear mushrooms (Auricularia auricula-judae): Assessing composition and antioxidant activity during fruiting body development |
| title_fullStr | Ultrasound-assisted extraction of phenolic compounds from ear mushrooms (Auricularia auricula-judae): Assessing composition and antioxidant activity during fruiting body development |
| title_full_unstemmed | Ultrasound-assisted extraction of phenolic compounds from ear mushrooms (Auricularia auricula-judae): Assessing composition and antioxidant activity during fruiting body development |
| title_short | Ultrasound-assisted extraction of phenolic compounds from ear mushrooms (Auricularia auricula-judae): Assessing composition and antioxidant activity during fruiting body development |
| title_sort | ultrasound assisted extraction of phenolic compounds from ear mushrooms auricularia auricula judae assessing composition and antioxidant activity during fruiting body development |
| topic | box-behnken design method development tpc antioxidant validation |
| url | https://www.aimspress.com/article/doi/10.3934/agrfood.2024059 |
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