Clinical, Biochemical and Molecular study of Mycoplasma haemocanis in Dogs in Southern Provinces of Iraq
The present study aimed to detection and identified the infection of Mycoplasma haemocanis in dogs through clinical manifestation, blood film, conventional PCR technique and sequences analysis. The total number of dogs examined clinically were one hundred, and 25 clinically healthy control in southe...
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College of Veterinary Medicine, University of Basrah, Iraq
2022-12-01
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| Series: | Basrah Journal of Veterinary Research |
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| Online Access: | https://bjvr.uobasrah.edu.iq/article_177418_eed5bb86c5cece5af76fc69c3b86478c.pdf |
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| author | Ali Radhi Israa Alsaad |
| author_facet | Ali Radhi Israa Alsaad |
| author_sort | Ali Radhi |
| collection | DOAJ |
| description | The present study aimed to detection and identified the infection of Mycoplasma haemocanis in dogs through clinical manifestation, blood film, conventional PCR technique and sequences analysis. The total number of dogs examined clinically were one hundred, and 25 clinically healthy control in southern Iraq provinces. Examined dogs showed various clinical signs. Blood samples were taking for hematological and biochemical analysis. 72 (72%) of blood smear were positive for Mycoplasma haemocanis, whereas 33(45.8%) positive based on conventional PCR technique. hematological examination in infected animals showed a significant decrease in mean of TRBC, Hb and PCV and significant increase (p>0.05) in the TLC. biochemical examination indicates a significant increase in AST, ALP and ALT in infected animals. The local Mycoplasma haemocanis were showed genetic variant related to NCBI-BLAST Mycoplasma haemocanis Turkey, Thailand, Brazil, and India isolates. In conclusion Mycoplasma haemocanis have been diagnosed in dogs in Basrah city with various clinical manifestation from in apparent to sever anemia, emphasized the infection using conventional PCR technique, and Phylogenetic analysis confirm the identification of Mycoplasma haemocanis as a new submission of local Iraq, with 99% identical to India, Brazil, Thailand and Turkey DNA sequence. |
| format | Article |
| id | doaj-art-7b13b06c73e54d0bb006ec9210e5bbd0 |
| institution | DOAJ |
| issn | 1813-8497 2410-8456 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | College of Veterinary Medicine, University of Basrah, Iraq |
| record_format | Article |
| series | Basrah Journal of Veterinary Research |
| spelling | doaj-art-7b13b06c73e54d0bb006ec9210e5bbd02025-08-20T03:09:55ZengCollege of Veterinary Medicine, University of Basrah, IraqBasrah Journal of Veterinary Research1813-84972410-84562022-12-0121S1445710.23975/bjvetr.2022.177418177418Clinical, Biochemical and Molecular study of Mycoplasma haemocanis in Dogs in Southern Provinces of IraqAli Radhi0Israa Alsaad11Ministry of Interior, Police Affairs Agency, Dhi-Qar Governorate Police Directorate, Department of Administration and Training of Police Dogs.Department of Internal and Preventive Medicine, College of Veterinary Medicine, University of BasrahThe present study aimed to detection and identified the infection of Mycoplasma haemocanis in dogs through clinical manifestation, blood film, conventional PCR technique and sequences analysis. The total number of dogs examined clinically were one hundred, and 25 clinically healthy control in southern Iraq provinces. Examined dogs showed various clinical signs. Blood samples were taking for hematological and biochemical analysis. 72 (72%) of blood smear were positive for Mycoplasma haemocanis, whereas 33(45.8%) positive based on conventional PCR technique. hematological examination in infected animals showed a significant decrease in mean of TRBC, Hb and PCV and significant increase (p>0.05) in the TLC. biochemical examination indicates a significant increase in AST, ALP and ALT in infected animals. The local Mycoplasma haemocanis were showed genetic variant related to NCBI-BLAST Mycoplasma haemocanis Turkey, Thailand, Brazil, and India isolates. In conclusion Mycoplasma haemocanis have been diagnosed in dogs in Basrah city with various clinical manifestation from in apparent to sever anemia, emphasized the infection using conventional PCR technique, and Phylogenetic analysis confirm the identification of Mycoplasma haemocanis as a new submission of local Iraq, with 99% identical to India, Brazil, Thailand and Turkey DNA sequence.https://bjvr.uobasrah.edu.iq/article_177418_eed5bb86c5cece5af76fc69c3b86478c.pdfmycoplasma haemocanisclinical signshematological changesbiochemical changes pcr technique |
| spellingShingle | Ali Radhi Israa Alsaad Clinical, Biochemical and Molecular study of Mycoplasma haemocanis in Dogs in Southern Provinces of Iraq Basrah Journal of Veterinary Research mycoplasma haemocanis clinical signs hematological changes biochemical changes pcr technique |
| title | Clinical, Biochemical and Molecular study of Mycoplasma haemocanis in Dogs in Southern Provinces of Iraq |
| title_full | Clinical, Biochemical and Molecular study of Mycoplasma haemocanis in Dogs in Southern Provinces of Iraq |
| title_fullStr | Clinical, Biochemical and Molecular study of Mycoplasma haemocanis in Dogs in Southern Provinces of Iraq |
| title_full_unstemmed | Clinical, Biochemical and Molecular study of Mycoplasma haemocanis in Dogs in Southern Provinces of Iraq |
| title_short | Clinical, Biochemical and Molecular study of Mycoplasma haemocanis in Dogs in Southern Provinces of Iraq |
| title_sort | clinical biochemical and molecular study of mycoplasma haemocanis in dogs in southern provinces of iraq |
| topic | mycoplasma haemocanis clinical signs hematological changes biochemical changes pcr technique |
| url | https://bjvr.uobasrah.edu.iq/article_177418_eed5bb86c5cece5af76fc69c3b86478c.pdf |
| work_keys_str_mv | AT aliradhi clinicalbiochemicalandmolecularstudyofmycoplasmahaemocanisindogsinsouthernprovincesofiraq AT israaalsaad clinicalbiochemicalandmolecularstudyofmycoplasmahaemocanisindogsinsouthernprovincesofiraq |