NanoSSIEFARL polymeric nanoparticles-based immunotherapeutic for the treatment of genital herpes
Abstract This study aimed to investigate the therapeutic potential of a nanoparticle formulation containing the immunodominant peptide SSIEFARL from Herpes simplex virus 2 glycoprotein B A against genital herpes. A nanoparticle formulation (NanoSSIEFARL) was engineered and characterized for its phys...
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Associação Brasileira de Polímeros
2025-04-01
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| Series: | Polímeros |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282025000200601&lng=en&tlng=en |
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| author | Renata Zorzetto Flávia Pires Peña Aline Cláudio de Oliveira Jayme de Castilhos Ferreira Neto Gabriel Tardin Mota Hilario Fernanda Teresa Bovi Frozza Marvin Paulo Lins Fernanda Poletto Marcelo Jung Eberhardt Pedro Roosevelt Torres Romão Tanira Alessandra Silveira Aguirre Luiz Carlos Rodrigues Junior |
| author_facet | Renata Zorzetto Flávia Pires Peña Aline Cláudio de Oliveira Jayme de Castilhos Ferreira Neto Gabriel Tardin Mota Hilario Fernanda Teresa Bovi Frozza Marvin Paulo Lins Fernanda Poletto Marcelo Jung Eberhardt Pedro Roosevelt Torres Romão Tanira Alessandra Silveira Aguirre Luiz Carlos Rodrigues Junior |
| author_sort | Renata Zorzetto |
| collection | DOAJ |
| description | Abstract This study aimed to investigate the therapeutic potential of a nanoparticle formulation containing the immunodominant peptide SSIEFARL from Herpes simplex virus 2 glycoprotein B A against genital herpes. A nanoparticle formulation (NanoSSIEFARL) was engineered and characterized for its physicochemical and immunomodulatory properties. NanoSSIEFARL displayed mean particle size of 212 ± 5 nm, polydispersity index of 0.12 ± 0.01 and zeta potential of -7.4 ± 2.5 mV, exhibiting spherical morphology. pH stability remained consistent over 30 days (day 0: 4.5 ± 0.5; day 30: 5.0 ± 0.5). A novel high-performance liquid chromatography method was validated for SSIEFARL quantification. Peptide association efficiency reached 98.3% ± 2.1, with 41.6% ± 4.4 peptide release from nanoparticles after 4 hours. Cytotoxicity assessment revealed cellular viability exceeding 90%, with macrophage uptake observed after 4 hours. Altogether, these results suggest that NanoSSIEFARL is a promising candidate for effective immunotherapy against genital herpes. |
| format | Article |
| id | doaj-art-7b1396e91b9e47ec9017cf15ef32607d |
| institution | DOAJ |
| issn | 1678-5169 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Associação Brasileira de Polímeros |
| record_format | Article |
| series | Polímeros |
| spelling | doaj-art-7b1396e91b9e47ec9017cf15ef32607d2025-08-20T03:11:47ZengAssociação Brasileira de PolímerosPolímeros1678-51692025-04-0135210.1590/0104-1428.20240042NanoSSIEFARL polymeric nanoparticles-based immunotherapeutic for the treatment of genital herpesRenata Zorzettohttps://orcid.org/0000-0003-3105-1014Flávia Pires Peñahttps://orcid.org/0009-0002-6877-4646Aline Cláudio de Oliveirahttps://orcid.org/0009-0006-3529-7803Jayme de Castilhos Ferreira Netohttps://orcid.org/0009-0009-5172-3771Gabriel Tardin Mota Hilariohttps://orcid.org/0000-0001-5314-2131Fernanda Teresa Bovi Frozzahttps://orcid.org/0000-0001-9718-7243Marvin Paulo Linshttps://orcid.org/0000-0003-4247-0345Fernanda Polettohttps://orcid.org/0000-0001-7217-0007Marcelo Jung Eberhardthttps://orcid.org/0000-0001-5139-6680Pedro Roosevelt Torres Romãohttps://orcid.org/0000-0002-1039-2509Tanira Alessandra Silveira Aguirrehttps://orcid.org/0000-0001-7178-6686Luiz Carlos Rodrigues Juniorhttps://orcid.org/0000-0002-5380-8930Abstract This study aimed to investigate the therapeutic potential of a nanoparticle formulation containing the immunodominant peptide SSIEFARL from Herpes simplex virus 2 glycoprotein B A against genital herpes. A nanoparticle formulation (NanoSSIEFARL) was engineered and characterized for its physicochemical and immunomodulatory properties. NanoSSIEFARL displayed mean particle size of 212 ± 5 nm, polydispersity index of 0.12 ± 0.01 and zeta potential of -7.4 ± 2.5 mV, exhibiting spherical morphology. pH stability remained consistent over 30 days (day 0: 4.5 ± 0.5; day 30: 5.0 ± 0.5). A novel high-performance liquid chromatography method was validated for SSIEFARL quantification. Peptide association efficiency reached 98.3% ± 2.1, with 41.6% ± 4.4 peptide release from nanoparticles after 4 hours. Cytotoxicity assessment revealed cellular viability exceeding 90%, with macrophage uptake observed after 4 hours. Altogether, these results suggest that NanoSSIEFARL is a promising candidate for effective immunotherapy against genital herpes.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282025000200601&lng=en&tlng=enbabassu oilgenital herpesHPLCpolymeric nanoparticle |
| spellingShingle | Renata Zorzetto Flávia Pires Peña Aline Cláudio de Oliveira Jayme de Castilhos Ferreira Neto Gabriel Tardin Mota Hilario Fernanda Teresa Bovi Frozza Marvin Paulo Lins Fernanda Poletto Marcelo Jung Eberhardt Pedro Roosevelt Torres Romão Tanira Alessandra Silveira Aguirre Luiz Carlos Rodrigues Junior NanoSSIEFARL polymeric nanoparticles-based immunotherapeutic for the treatment of genital herpes Polímeros babassu oil genital herpes HPLC polymeric nanoparticle |
| title | NanoSSIEFARL polymeric nanoparticles-based immunotherapeutic for the treatment of genital herpes |
| title_full | NanoSSIEFARL polymeric nanoparticles-based immunotherapeutic for the treatment of genital herpes |
| title_fullStr | NanoSSIEFARL polymeric nanoparticles-based immunotherapeutic for the treatment of genital herpes |
| title_full_unstemmed | NanoSSIEFARL polymeric nanoparticles-based immunotherapeutic for the treatment of genital herpes |
| title_short | NanoSSIEFARL polymeric nanoparticles-based immunotherapeutic for the treatment of genital herpes |
| title_sort | nanossiefarl polymeric nanoparticles based immunotherapeutic for the treatment of genital herpes |
| topic | babassu oil genital herpes HPLC polymeric nanoparticle |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282025000200601&lng=en&tlng=en |
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