Development and Application of a Multiplex Real-Time TaqMan qPCR Assay for the Simultaneous Detection of African Swine Fever Virus, Classical Swine Fever Virus, Porcine Reproductive and Respiratory Syndrome Virus, Pseudorabies Virus, and Porcine Circovirus Type 2

Development and Application of a Multiplex Real-Time TaqMan qPCR Assay for the Simultaneous Detection of African Swine Fever Virus, Classical Swine Fever Virus, Porcine Reproductive and Respiratory Syndrome Virus, Pseudorabies Virus, and Porcine Circovirus Type 2

Since its emergence in China in 2018, African swine fever virus (ASFV) has posed a severe threat to the pig farming industry due to its high transmissibility and mortality rate. The clinical signs of ASFV infection often overlap with those caused by other swine viruses such as classical swine fever...

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Bibliographic Details
Main Authors: Dongdong Yin, Shuangshuang Xu, Yayun Liu, Hao Guo, Mengdie Lan, Lei Yin, Jieru Wang, Yin Dai, Xuehuai Shen, Kai Zhan, Xiaocheng Pan
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Microorganisms
Subjects:
African swine fever virus
classical swine fever virus
pseudorabies virus
multiplex qPCR
differential diagnosis
TaqMan assay
Online Access:https://www.mdpi.com/2076-2607/13/7/1573
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