Metallothionein 3 attenuated the apoptosis of neurons in the CA1 region of the hippocampus in the senescence-accelerated mouse/PRONE8 (SAMP8)
OBJECTIVE: Metallothionein 3 (MT-3) has been shown to protect against apoptotic neuronal death in the brains of patients with Alzheimer's disease. Zinc is a potent inhibitor of caspase-3 and its deficiency was found to promote apoptosis. Here, we measured the zinc and copper content in the brai...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Thieme Revinter Publicações
2011-02-01
|
| Series: | Arquivos de Neuro-Psiquiatria |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2011000100020&lng=en&tlng=en |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850220495262187520 |
|---|---|
| author | Feiyu Ma Hu Wang Bin Chen Feng Wang Haixiong Xu |
| author_facet | Feiyu Ma Hu Wang Bin Chen Feng Wang Haixiong Xu |
| author_sort | Feiyu Ma |
| collection | DOAJ |
| description | OBJECTIVE: Metallothionein 3 (MT-3) has been shown to protect against apoptotic neuronal death in the brains of patients with Alzheimer's disease. Zinc is a potent inhibitor of caspase-3 and its deficiency was found to promote apoptosis. Here, we measured the zinc and copper content in the brains of senescence-accelerated mouse/PRONE8 (SAMP8) and sought to investigate the effect of MT-3 on the apoptosis of neurons in the hippocampal CA1 region of these mice. METHOD: The zinc and copper content in the brain samples of SAMP8 and normal control SAMR1 mice were determined using an atomic absorption spectrophotometer. The mice were administered intraperitoneally for four weeks with MT-3 or MT1 and thereafter apoptosis was measured using the TUNEL method and the expression of anti-apoptotic protein Bcl-2 and proapoptotic protein Bax was examined by immunohistochemistry. RESULTS: Compared with that in SMAR1 mice, the content of zinc in the brains of SAMP8 mice was significantly reduced (P<0.05). Moreover, significant levels of apoptosis of neurons were observed in the hippocampus of SAMP8 mice, which, compared with those in SMAR1 mice, also showed significantly lower levels of Bcl-2 and higher levels of Bax (P<0.05). MT-3 increased zinc concentration in the hippocampus of SAMP8 mice and also significantly decreased apoptosis in these neurons dose-dependently and increased the levels of Bcl-2 and decreased the levels of Bax. CONCLUSION: MT-3 could attenuate apoptotic neuron death in the hippocampus of SAMP8, suggesting that the protein may lessen the development of neurodegeneration. |
| format | Article |
| id | doaj-art-7b11aa0e3eca44c6b18eb307c93a674b |
| institution | OA Journals |
| issn | 1678-4227 |
| language | English |
| publishDate | 2011-02-01 |
| publisher | Thieme Revinter Publicações |
| record_format | Article |
| series | Arquivos de Neuro-Psiquiatria |
| spelling | doaj-art-7b11aa0e3eca44c6b18eb307c93a674b2025-08-20T02:07:02ZengThieme Revinter PublicaçõesArquivos de Neuro-Psiquiatria1678-42272011-02-0169110511110.1590/S0004-282X2011000100020S0004-282X2011000100020Metallothionein 3 attenuated the apoptosis of neurons in the CA1 region of the hippocampus in the senescence-accelerated mouse/PRONE8 (SAMP8)Feiyu Ma0Hu Wang1Bin Chen2Feng Wang3Haixiong Xu4Sun Yat-sen UniversityShantou University Medical CollegeShantou University Medical CollegeXiamen UniversitySun Yat-sen UniversityOBJECTIVE: Metallothionein 3 (MT-3) has been shown to protect against apoptotic neuronal death in the brains of patients with Alzheimer's disease. Zinc is a potent inhibitor of caspase-3 and its deficiency was found to promote apoptosis. Here, we measured the zinc and copper content in the brains of senescence-accelerated mouse/PRONE8 (SAMP8) and sought to investigate the effect of MT-3 on the apoptosis of neurons in the hippocampal CA1 region of these mice. METHOD: The zinc and copper content in the brain samples of SAMP8 and normal control SAMR1 mice were determined using an atomic absorption spectrophotometer. The mice were administered intraperitoneally for four weeks with MT-3 or MT1 and thereafter apoptosis was measured using the TUNEL method and the expression of anti-apoptotic protein Bcl-2 and proapoptotic protein Bax was examined by immunohistochemistry. RESULTS: Compared with that in SMAR1 mice, the content of zinc in the brains of SAMP8 mice was significantly reduced (P<0.05). Moreover, significant levels of apoptosis of neurons were observed in the hippocampus of SAMP8 mice, which, compared with those in SMAR1 mice, also showed significantly lower levels of Bcl-2 and higher levels of Bax (P<0.05). MT-3 increased zinc concentration in the hippocampus of SAMP8 mice and also significantly decreased apoptosis in these neurons dose-dependently and increased the levels of Bcl-2 and decreased the levels of Bax. CONCLUSION: MT-3 could attenuate apoptotic neuron death in the hippocampus of SAMP8, suggesting that the protein may lessen the development of neurodegeneration.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2011000100020&lng=en&tlng=enmetalotioneína 3 (MT-3)SAMP8neurodegeneraçãoapoptose |
| spellingShingle | Feiyu Ma Hu Wang Bin Chen Feng Wang Haixiong Xu Metallothionein 3 attenuated the apoptosis of neurons in the CA1 region of the hippocampus in the senescence-accelerated mouse/PRONE8 (SAMP8) Arquivos de Neuro-Psiquiatria metalotioneína 3 (MT-3) SAMP8 neurodegeneração apoptose |
| title | Metallothionein 3 attenuated the apoptosis of neurons in the CA1 region of the hippocampus in the senescence-accelerated mouse/PRONE8 (SAMP8) |
| title_full | Metallothionein 3 attenuated the apoptosis of neurons in the CA1 region of the hippocampus in the senescence-accelerated mouse/PRONE8 (SAMP8) |
| title_fullStr | Metallothionein 3 attenuated the apoptosis of neurons in the CA1 region of the hippocampus in the senescence-accelerated mouse/PRONE8 (SAMP8) |
| title_full_unstemmed | Metallothionein 3 attenuated the apoptosis of neurons in the CA1 region of the hippocampus in the senescence-accelerated mouse/PRONE8 (SAMP8) |
| title_short | Metallothionein 3 attenuated the apoptosis of neurons in the CA1 region of the hippocampus in the senescence-accelerated mouse/PRONE8 (SAMP8) |
| title_sort | metallothionein 3 attenuated the apoptosis of neurons in the ca1 region of the hippocampus in the senescence accelerated mouse prone8 samp8 |
| topic | metalotioneína 3 (MT-3) SAMP8 neurodegeneração apoptose |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2011000100020&lng=en&tlng=en |
| work_keys_str_mv | AT feiyuma metallothionein3attenuatedtheapoptosisofneuronsintheca1regionofthehippocampusinthesenescenceacceleratedmouseprone8samp8 AT huwang metallothionein3attenuatedtheapoptosisofneuronsintheca1regionofthehippocampusinthesenescenceacceleratedmouseprone8samp8 AT binchen metallothionein3attenuatedtheapoptosisofneuronsintheca1regionofthehippocampusinthesenescenceacceleratedmouseprone8samp8 AT fengwang metallothionein3attenuatedtheapoptosisofneuronsintheca1regionofthehippocampusinthesenescenceacceleratedmouseprone8samp8 AT haixiongxu metallothionein3attenuatedtheapoptosisofneuronsintheca1regionofthehippocampusinthesenescenceacceleratedmouseprone8samp8 |