Proteomic analysis reveals dynamic expression related to malondialdehyde in cassava in response to cassava bacterial blight
Abstract Cassava bacterial blight (CBB) significantly affects cassava production in Thailand. Understanding tolerant cultivar responses can reveal key disease resistance mechanisms. This study identified differentially abundant proteins between the tolerant ‘Rayong72’ (R72) and susceptible ‘Hanatee’...
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-10051-9 |
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| author | Chotiros Phaisomboon Supajit Sraphet Nattaya Srisawad Piengtawan Tappiban Sittiruk Roytrakul Duncan R. Smith Kanokporn Triwitayakorn |
| author_facet | Chotiros Phaisomboon Supajit Sraphet Nattaya Srisawad Piengtawan Tappiban Sittiruk Roytrakul Duncan R. Smith Kanokporn Triwitayakorn |
| author_sort | Chotiros Phaisomboon |
| collection | DOAJ |
| description | Abstract Cassava bacterial blight (CBB) significantly affects cassava production in Thailand. Understanding tolerant cultivar responses can reveal key disease resistance mechanisms. This study identified differentially abundant proteins between the tolerant ‘Rayong72’ (R72) and susceptible ‘Hanatee’ (HN) cassava cultivars, with quantitative PCR validating mRNA-level changes. Several upregulated proteins in the R72 cultivar were linked to disease progression and defense mechanisms. Correlations between phenotypic symptoms, including malondialdehyde (MDA) levels and protein expression trends, were observed. A higher MDA level, associated with cell damage, correlated with severe symptoms in the HN at the infection site. However, in R72, despite higher MDA levels in leaves compared to HN, oxidative stress-related proteins were also upregulated. Notably, proteins involved in NAD/NADP biosynthesis (QNS), glutathione-based redox regulation (GSS), and MAPK signaling (MAPKKK18) were enriched in the tolerant cultivar, suggesting distinct reactive oxygen species (ROS) regulation and contributing to differential resistance. Taken together, these findings highlight the role of MDA and coenzyme-related proteins in cassava’s defense responses to bacterial infections, emphasizing their importance in stress regulation. This study provides molecular insights into cassava-CBB interactions and underscores the potential of proteomic analysis in advancing plant disease resistance research as well as aiding breeding programs. |
| format | Article |
| id | doaj-art-7b07720bd69c4ddc807179957e940488 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-7b07720bd69c4ddc807179957e9404882025-08-20T03:05:26ZengNature PortfolioScientific Reports2045-23222025-07-0115111510.1038/s41598-025-10051-9Proteomic analysis reveals dynamic expression related to malondialdehyde in cassava in response to cassava bacterial blightChotiros Phaisomboon0Supajit Sraphet1Nattaya Srisawad2Piengtawan Tappiban3Sittiruk Roytrakul4Duncan R. Smith5Kanokporn Triwitayakorn6Institute of Molecular Biosciences, Mahidol UniversityInstitute of Molecular Biosciences, Mahidol UniversityInstitute of Molecular Biosciences, Mahidol UniversityInstitute of Molecular Biosciences, Mahidol UniversityFunctional Proteomics Technology Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development AgencyInstitute of Molecular Biosciences, Mahidol UniversityInstitute of Molecular Biosciences, Mahidol UniversityAbstract Cassava bacterial blight (CBB) significantly affects cassava production in Thailand. Understanding tolerant cultivar responses can reveal key disease resistance mechanisms. This study identified differentially abundant proteins between the tolerant ‘Rayong72’ (R72) and susceptible ‘Hanatee’ (HN) cassava cultivars, with quantitative PCR validating mRNA-level changes. Several upregulated proteins in the R72 cultivar were linked to disease progression and defense mechanisms. Correlations between phenotypic symptoms, including malondialdehyde (MDA) levels and protein expression trends, were observed. A higher MDA level, associated with cell damage, correlated with severe symptoms in the HN at the infection site. However, in R72, despite higher MDA levels in leaves compared to HN, oxidative stress-related proteins were also upregulated. Notably, proteins involved in NAD/NADP biosynthesis (QNS), glutathione-based redox regulation (GSS), and MAPK signaling (MAPKKK18) were enriched in the tolerant cultivar, suggesting distinct reactive oxygen species (ROS) regulation and contributing to differential resistance. Taken together, these findings highlight the role of MDA and coenzyme-related proteins in cassava’s defense responses to bacterial infections, emphasizing their importance in stress regulation. This study provides molecular insights into cassava-CBB interactions and underscores the potential of proteomic analysis in advancing plant disease resistance research as well as aiding breeding programs.https://doi.org/10.1038/s41598-025-10051-9Manihot esculentaPlant defense mechanismsStem puncture inoculationXanthomonas axonopodis pv. manihotis |
| spellingShingle | Chotiros Phaisomboon Supajit Sraphet Nattaya Srisawad Piengtawan Tappiban Sittiruk Roytrakul Duncan R. Smith Kanokporn Triwitayakorn Proteomic analysis reveals dynamic expression related to malondialdehyde in cassava in response to cassava bacterial blight Scientific Reports Manihot esculenta Plant defense mechanisms Stem puncture inoculation Xanthomonas axonopodis pv. manihotis |
| title | Proteomic analysis reveals dynamic expression related to malondialdehyde in cassava in response to cassava bacterial blight |
| title_full | Proteomic analysis reveals dynamic expression related to malondialdehyde in cassava in response to cassava bacterial blight |
| title_fullStr | Proteomic analysis reveals dynamic expression related to malondialdehyde in cassava in response to cassava bacterial blight |
| title_full_unstemmed | Proteomic analysis reveals dynamic expression related to malondialdehyde in cassava in response to cassava bacterial blight |
| title_short | Proteomic analysis reveals dynamic expression related to malondialdehyde in cassava in response to cassava bacterial blight |
| title_sort | proteomic analysis reveals dynamic expression related to malondialdehyde in cassava in response to cassava bacterial blight |
| topic | Manihot esculenta Plant defense mechanisms Stem puncture inoculation Xanthomonas axonopodis pv. manihotis |
| url | https://doi.org/10.1038/s41598-025-10051-9 |
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