Multiparameter Flow Cytometric Analysis of the Conventional and Monocyte-Derived DC Compartment in the Murine Spleen
Dendritic cells (DCs) are present in almost all tissues, where they act as sentinels involved in innate recognition and the initiation of adaptive immune responses. The DC family consists of several cell lineages that are heterogenous in their development, phenotype, and function. Within these DC li...
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MDPI AG
2024-11-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/12/11/1294 |
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| author | Ronald A. Backer Hans Christian Probst Björn E. Clausen |
| author_facet | Ronald A. Backer Hans Christian Probst Björn E. Clausen |
| author_sort | Ronald A. Backer |
| collection | DOAJ |
| description | Dendritic cells (DCs) are present in almost all tissues, where they act as sentinels involved in innate recognition and the initiation of adaptive immune responses. The DC family consists of several cell lineages that are heterogenous in their development, phenotype, and function. Within these DC lineages, further subdivisions exist, resulting in smaller, less characterized subpopulations, each with its unique immunomodulatory capabilities. Given the interest in utilizing DC for experimental studies and for vaccination purposes, it becomes increasingly crucial to thoroughly classify and characterize these diverse DC subpopulations. This understanding is vital for comprehending their relative contribution to the initiation, regulation, and propagation of immune responses. To facilitate such investigation, we here provide an easy and ready-to-use multicolor flow cytometry staining panel for the analysis of conventional DC, plasmacytoid DC, and monocyte-derived DC populations isolated from mouse spleens. This adaptable panel can be easily customized for the analysis of other tissue-specific DC populations, providing a valuable tool for DC research. |
| format | Article |
| id | doaj-art-7b007cc834da4a56ba4fb9a1b1ce1c39 |
| institution | OA Journals |
| issn | 2076-393X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj-art-7b007cc834da4a56ba4fb9a1b1ce1c392025-08-20T02:27:41ZengMDPI AGVaccines2076-393X2024-11-011211129410.3390/vaccines12111294Multiparameter Flow Cytometric Analysis of the Conventional and Monocyte-Derived DC Compartment in the Murine SpleenRonald A. Backer0Hans Christian Probst1Björn E. Clausen2Institute for Molecular Medicine, Paul Klein Center for Immune Intervention, University Medical Center of the Johannes Gutenberg-University Mainz, 55131 Mainz, GermanyResearch Center for Immunotherapy (FZI), University Medical Center of the Johannes Gutenberg-University Mainz, 55131 Mainz, GermanyInstitute for Molecular Medicine, Paul Klein Center for Immune Intervention, University Medical Center of the Johannes Gutenberg-University Mainz, 55131 Mainz, GermanyDendritic cells (DCs) are present in almost all tissues, where they act as sentinels involved in innate recognition and the initiation of adaptive immune responses. The DC family consists of several cell lineages that are heterogenous in their development, phenotype, and function. Within these DC lineages, further subdivisions exist, resulting in smaller, less characterized subpopulations, each with its unique immunomodulatory capabilities. Given the interest in utilizing DC for experimental studies and for vaccination purposes, it becomes increasingly crucial to thoroughly classify and characterize these diverse DC subpopulations. This understanding is vital for comprehending their relative contribution to the initiation, regulation, and propagation of immune responses. To facilitate such investigation, we here provide an easy and ready-to-use multicolor flow cytometry staining panel for the analysis of conventional DC, plasmacytoid DC, and monocyte-derived DC populations isolated from mouse spleens. This adaptable panel can be easily customized for the analysis of other tissue-specific DC populations, providing a valuable tool for DC research.https://www.mdpi.com/2076-393X/12/11/1294dendritic celldendritic cell subpopulationsmacrophagesmonocytesmulticolor flow cytometrymyeloid cells |
| spellingShingle | Ronald A. Backer Hans Christian Probst Björn E. Clausen Multiparameter Flow Cytometric Analysis of the Conventional and Monocyte-Derived DC Compartment in the Murine Spleen Vaccines dendritic cell dendritic cell subpopulations macrophages monocytes multicolor flow cytometry myeloid cells |
| title | Multiparameter Flow Cytometric Analysis of the Conventional and Monocyte-Derived DC Compartment in the Murine Spleen |
| title_full | Multiparameter Flow Cytometric Analysis of the Conventional and Monocyte-Derived DC Compartment in the Murine Spleen |
| title_fullStr | Multiparameter Flow Cytometric Analysis of the Conventional and Monocyte-Derived DC Compartment in the Murine Spleen |
| title_full_unstemmed | Multiparameter Flow Cytometric Analysis of the Conventional and Monocyte-Derived DC Compartment in the Murine Spleen |
| title_short | Multiparameter Flow Cytometric Analysis of the Conventional and Monocyte-Derived DC Compartment in the Murine Spleen |
| title_sort | multiparameter flow cytometric analysis of the conventional and monocyte derived dc compartment in the murine spleen |
| topic | dendritic cell dendritic cell subpopulations macrophages monocytes multicolor flow cytometry myeloid cells |
| url | https://www.mdpi.com/2076-393X/12/11/1294 |
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