Effect of Perfluoran on the Morphology of a Red Blood Cell in Acute Blood Loss
Objective: to evaluate the cytoprotective effect of perfluoran when pre-administered in massive blood loss. Material and methods. Experiments were performed on 21 nembutal-anesthetized rats. Acute blood loss from the rat caudal artery served as a model for a terminal state. Perfluoran had been injec...
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| Format: | Article |
| Language: | English |
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Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia
2013-10-01
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| Series: | Общая реаниматология |
| Online Access: | https://www.reanimatology.com/rmt/article/view/93 |
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| author | V. V. Moroz I. S. Novoderzhkina E. M. Antoshina A. V. Afanasyev |
| author_facet | V. V. Moroz I. S. Novoderzhkina E. M. Antoshina A. V. Afanasyev |
| author_sort | V. V. Moroz |
| collection | DOAJ |
| description | Objective: to evaluate the cytoprotective effect of perfluoran when pre-administered in massive blood loss. Material and methods. Experiments were performed on 21 nembutal-anesthetized rats. Acute blood loss from the rat caudal artery served as a model for a terminal state. Perfluoran had been injected 45 minutes prior to blood loss. Morphological and morphome-tric analyses of monolayer dried unstained blood smear slides were made using an Olympus BX-50 light microscope at a magnification of 1000 (10X100). Cytometry was carried out using Image scope color software. Results. In the control group of experiments, i. e. without perfluoran preadministration, at 5 minutes of blood loss, the circulating blood showed only 20% of discoid forms; the other forms of the red blood cells were grades 1-to-3 transformation stomatocytes. By 60 minutes of hypotension, there was a decrease in stomatocyte counts to 5.2% and a change in the ratio of circulating red blood cell populations. The number of smaller, i.e. senescent, cells was increased and that of large cells was reduced. The preventive administration of perfluoran in small amounts playing no prominent role in the delivery of oxygen and the alteration of circulating blood volume, substantially changed the ratio of different shapes and sizes of erythrocytes, increased their average diameter, and stabilized the membrane of a red blood cell. Conclusion. The preadministration of low-dose perfluoran before blood loss prevents deformational stress, increases the surface of a red blood cell, and stabilizes its membrane. Key words: blood loss, perfluoran, red blood cell, stomatocyte, deformation stress, rheology. |
| format | Article |
| id | doaj-art-7afe25cf2bdf43c1a9282e82c948dc49 |
| institution | Kabale University |
| issn | 1813-9779 2411-7110 |
| language | English |
| publishDate | 2013-10-01 |
| publisher | Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia |
| record_format | Article |
| series | Общая реаниматология |
| spelling | doaj-art-7afe25cf2bdf43c1a9282e82c948dc492025-08-20T03:59:40ZengFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, RussiaОбщая реаниматология1813-97792411-71102013-10-019510.15360/1813-9779-2013-5-593Effect of Perfluoran on the Morphology of a Red Blood Cell in Acute Blood LossV. V. MorozI. S. NovoderzhkinaE. M. AntoshinaA. V. AfanasyevObjective: to evaluate the cytoprotective effect of perfluoran when pre-administered in massive blood loss. Material and methods. Experiments were performed on 21 nembutal-anesthetized rats. Acute blood loss from the rat caudal artery served as a model for a terminal state. Perfluoran had been injected 45 minutes prior to blood loss. Morphological and morphome-tric analyses of monolayer dried unstained blood smear slides were made using an Olympus BX-50 light microscope at a magnification of 1000 (10X100). Cytometry was carried out using Image scope color software. Results. In the control group of experiments, i. e. without perfluoran preadministration, at 5 minutes of blood loss, the circulating blood showed only 20% of discoid forms; the other forms of the red blood cells were grades 1-to-3 transformation stomatocytes. By 60 minutes of hypotension, there was a decrease in stomatocyte counts to 5.2% and a change in the ratio of circulating red blood cell populations. The number of smaller, i.e. senescent, cells was increased and that of large cells was reduced. The preventive administration of perfluoran in small amounts playing no prominent role in the delivery of oxygen and the alteration of circulating blood volume, substantially changed the ratio of different shapes and sizes of erythrocytes, increased their average diameter, and stabilized the membrane of a red blood cell. Conclusion. The preadministration of low-dose perfluoran before blood loss prevents deformational stress, increases the surface of a red blood cell, and stabilizes its membrane. Key words: blood loss, perfluoran, red blood cell, stomatocyte, deformation stress, rheology.https://www.reanimatology.com/rmt/article/view/93 |
| spellingShingle | V. V. Moroz I. S. Novoderzhkina E. M. Antoshina A. V. Afanasyev Effect of Perfluoran on the Morphology of a Red Blood Cell in Acute Blood Loss Общая реаниматология |
| title | Effect of Perfluoran on the Morphology of a Red Blood Cell in Acute Blood Loss |
| title_full | Effect of Perfluoran on the Morphology of a Red Blood Cell in Acute Blood Loss |
| title_fullStr | Effect of Perfluoran on the Morphology of a Red Blood Cell in Acute Blood Loss |
| title_full_unstemmed | Effect of Perfluoran on the Morphology of a Red Blood Cell in Acute Blood Loss |
| title_short | Effect of Perfluoran on the Morphology of a Red Blood Cell in Acute Blood Loss |
| title_sort | effect of perfluoran on the morphology of a red blood cell in acute blood loss |
| url | https://www.reanimatology.com/rmt/article/view/93 |
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