Low-Intensity Light-Responsive Anticancer Activity of Platinum(II) Complex Nanocolloids on 2D and 3D In Vitro Cancer Cell Model
This study aimed to evaluate the therapeutic efficacy of low-intensity visible light responsive nanocolloids of a Pt-based drug using a 2D and three-dimensional (3D) in vitro cancer cell model. Biocompatible and biodegradable polymeric nanocolloids, obtained using the ultrasonication method coupled...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2022/9571217 |
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| author | Viviana Vergaro Francesca Baldassarre Federica De Castro Danilo Migoni Maria Michela Dell’Anna Piero Mastrorilli Francesco Paolo Fanizzi Giuseppe Ciccarella |
| author_facet | Viviana Vergaro Francesca Baldassarre Federica De Castro Danilo Migoni Maria Michela Dell’Anna Piero Mastrorilli Francesco Paolo Fanizzi Giuseppe Ciccarella |
| author_sort | Viviana Vergaro |
| collection | DOAJ |
| description | This study aimed to evaluate the therapeutic efficacy of low-intensity visible light responsive nanocolloids of a Pt-based drug using a 2D and three-dimensional (3D) in vitro cancer cell model. Biocompatible and biodegradable polymeric nanocolloids, obtained using the ultrasonication method coupled with Layer by Layer technology, were characterized in terms of size (100 ± 20 nm), physical stability, drug loading (78%), and photoactivation through spectroscopy studies. The in vitro biological effects were assessed in terms of efficacy, apoptosis induction, and DNA-Pt adducts formation. Biological experiments were performed both in dark and under visible light irradiation conditions, exploiting the complex photochemical properties. The light-stimuli responsive nanoformulation gave a significant enhancement in drug bioactivity. This allowed us to achieve satisfying results by using nanomolar drug concentration (50 nM), which was ineffective in darkness condition. Furthermore, our nanocolloids were validated in 3D in vitro spheroids using confocal microscopy and cytofluorimetric assay to compare their behavior on culture in 2D monolayers. The obtained results confirmed that these nanocolloids are promising tools for delivering Pt-based drugs. |
| format | Article |
| id | doaj-art-7af532fd3520495aace0ac9790310d74 |
| institution | Kabale University |
| issn | 1687-479X |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-7af532fd3520495aace0ac9790310d742025-02-03T01:22:25ZengWileyBioinorganic Chemistry and Applications1687-479X2022-01-01202210.1155/2022/9571217Low-Intensity Light-Responsive Anticancer Activity of Platinum(II) Complex Nanocolloids on 2D and 3D In Vitro Cancer Cell ModelViviana Vergaro0Francesca Baldassarre1Federica De Castro2Danilo Migoni3Maria Michela Dell’Anna4Piero Mastrorilli5Francesco Paolo Fanizzi6Giuseppe Ciccarella7Institute of NanotechnologyInstitute of NanotechnologyBiological and Environmental Sciences DepartmentBiological and Environmental Sciences DepartmentDICATEChDICATEChBiological and Environmental Sciences DepartmentInstitute of NanotechnologyThis study aimed to evaluate the therapeutic efficacy of low-intensity visible light responsive nanocolloids of a Pt-based drug using a 2D and three-dimensional (3D) in vitro cancer cell model. Biocompatible and biodegradable polymeric nanocolloids, obtained using the ultrasonication method coupled with Layer by Layer technology, were characterized in terms of size (100 ± 20 nm), physical stability, drug loading (78%), and photoactivation through spectroscopy studies. The in vitro biological effects were assessed in terms of efficacy, apoptosis induction, and DNA-Pt adducts formation. Biological experiments were performed both in dark and under visible light irradiation conditions, exploiting the complex photochemical properties. The light-stimuli responsive nanoformulation gave a significant enhancement in drug bioactivity. This allowed us to achieve satisfying results by using nanomolar drug concentration (50 nM), which was ineffective in darkness condition. Furthermore, our nanocolloids were validated in 3D in vitro spheroids using confocal microscopy and cytofluorimetric assay to compare their behavior on culture in 2D monolayers. The obtained results confirmed that these nanocolloids are promising tools for delivering Pt-based drugs.http://dx.doi.org/10.1155/2022/9571217 |
| spellingShingle | Viviana Vergaro Francesca Baldassarre Federica De Castro Danilo Migoni Maria Michela Dell’Anna Piero Mastrorilli Francesco Paolo Fanizzi Giuseppe Ciccarella Low-Intensity Light-Responsive Anticancer Activity of Platinum(II) Complex Nanocolloids on 2D and 3D In Vitro Cancer Cell Model Bioinorganic Chemistry and Applications |
| title | Low-Intensity Light-Responsive Anticancer Activity of Platinum(II) Complex Nanocolloids on 2D and 3D In Vitro Cancer Cell Model |
| title_full | Low-Intensity Light-Responsive Anticancer Activity of Platinum(II) Complex Nanocolloids on 2D and 3D In Vitro Cancer Cell Model |
| title_fullStr | Low-Intensity Light-Responsive Anticancer Activity of Platinum(II) Complex Nanocolloids on 2D and 3D In Vitro Cancer Cell Model |
| title_full_unstemmed | Low-Intensity Light-Responsive Anticancer Activity of Platinum(II) Complex Nanocolloids on 2D and 3D In Vitro Cancer Cell Model |
| title_short | Low-Intensity Light-Responsive Anticancer Activity of Platinum(II) Complex Nanocolloids on 2D and 3D In Vitro Cancer Cell Model |
| title_sort | low intensity light responsive anticancer activity of platinum ii complex nanocolloids on 2d and 3d in vitro cancer cell model |
| url | http://dx.doi.org/10.1155/2022/9571217 |
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