Halotolerance of Phytoplankton and Invasion Success of Nostocalean Cyanobacteria Under Freshwater Salinization

Disturbed ecosystems are particularly susceptible to biological invasions. Increasing freshwater salinization, caused by anthropogenic factors, can alter the phytoplankton community and favour newly arrived halotolerant species. This study investigates the halotolerance of four Nostocalean cyanobact...

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Main Authors: Izabelė Šuikaitė, Gabrielė Šiurkutė, Robert Ptacnik, Judita Koreivienė
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Microorganisms
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Online Access:https://www.mdpi.com/2076-2607/13/6/1378
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author Izabelė Šuikaitė
Gabrielė Šiurkutė
Robert Ptacnik
Judita Koreivienė
author_facet Izabelė Šuikaitė
Gabrielė Šiurkutė
Robert Ptacnik
Judita Koreivienė
author_sort Izabelė Šuikaitė
collection DOAJ
description Disturbed ecosystems are particularly susceptible to biological invasions. Increasing freshwater salinization, caused by anthropogenic factors, can alter the phytoplankton community and favour newly arrived halotolerant species. This study investigates the halotolerance of four Nostocalean cyanobacterial species—the native to Europe, <i>Aphanizomenon gracile</i>, and alien <i>Chrysosporum bergii</i>, <i>Cuspidothrix issatschenkoi</i>, and <i>Sphaerospermopsis aphanizomenoides</i>—using monoculture experiments under varying NaCl concentrations. Additionally, we performed two microcosm experiments to explore shifts in biodiversity in freshwater phytoplankton communities sourced from artificial reservoirs and assess their susceptibility to cyanobacterial invasion under salinity stress. Results showed that all Nostocalean cyanobacteria were halotolerant under mild salinities (up to 1 g/L NaCl), with <i>Chrysosporum bergii</i> and <i>Sphaerospermopsis aphanizomenoides</i> demonstrating the most salt tolerance. In the microcosm experiment, changes in community composition were driven by the halotolerance of dominant groups. Water body 1, dominated by Bacillariophytina, reduced its biomass of phytoplankton at high salinity (5 g/L NaCl), while water body 2, dominated by Chlorophytina, remained stable regardless of disturbance. Both cyanobacteria successfully invaded both halotolerant and halosensitive communities, increasing their dominance as salinity rose. Our findings suggest that anthropogenic stressors such as freshwater salinization can alter the phytoplankton community and increase a competitive advantage to certain taxa, including widespread alien cyanobacteria, potentially promoting invasions and bloom formation.
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spelling doaj-art-7aec74e58e974764b643852cf76971e42025-08-20T02:21:14ZengMDPI AGMicroorganisms2076-26072025-06-01136137810.3390/microorganisms13061378Halotolerance of Phytoplankton and Invasion Success of Nostocalean Cyanobacteria Under Freshwater SalinizationIzabelė Šuikaitė0Gabrielė Šiurkutė1Robert Ptacnik2Judita Koreivienė3Laboratory of Algology and Microorganisms Ecology, State Scientific Research Institute Nature Research Centre, Akademijos St. 2, LT-08412 Vilnius, LithuaniaLaboratory of Algology and Microorganisms Ecology, State Scientific Research Institute Nature Research Centre, Akademijos St. 2, LT-08412 Vilnius, LithuaniaFaculty of Technology, Natural Sciences and Maritime Sciences, University of South-Eastern Norway, Lærerskoleveien 40, 3679 Notodden, NorwayLaboratory of Algology and Microorganisms Ecology, State Scientific Research Institute Nature Research Centre, Akademijos St. 2, LT-08412 Vilnius, LithuaniaDisturbed ecosystems are particularly susceptible to biological invasions. Increasing freshwater salinization, caused by anthropogenic factors, can alter the phytoplankton community and favour newly arrived halotolerant species. This study investigates the halotolerance of four Nostocalean cyanobacterial species—the native to Europe, <i>Aphanizomenon gracile</i>, and alien <i>Chrysosporum bergii</i>, <i>Cuspidothrix issatschenkoi</i>, and <i>Sphaerospermopsis aphanizomenoides</i>—using monoculture experiments under varying NaCl concentrations. Additionally, we performed two microcosm experiments to explore shifts in biodiversity in freshwater phytoplankton communities sourced from artificial reservoirs and assess their susceptibility to cyanobacterial invasion under salinity stress. Results showed that all Nostocalean cyanobacteria were halotolerant under mild salinities (up to 1 g/L NaCl), with <i>Chrysosporum bergii</i> and <i>Sphaerospermopsis aphanizomenoides</i> demonstrating the most salt tolerance. In the microcosm experiment, changes in community composition were driven by the halotolerance of dominant groups. Water body 1, dominated by Bacillariophytina, reduced its biomass of phytoplankton at high salinity (5 g/L NaCl), while water body 2, dominated by Chlorophytina, remained stable regardless of disturbance. Both cyanobacteria successfully invaded both halotolerant and halosensitive communities, increasing their dominance as salinity rose. Our findings suggest that anthropogenic stressors such as freshwater salinization can alter the phytoplankton community and increase a competitive advantage to certain taxa, including widespread alien cyanobacteria, potentially promoting invasions and bloom formation.https://www.mdpi.com/2076-2607/13/6/1378microbial invasioncyanobacteriahalotolerancealien speciesharmful algaedisturbed ecosystems
spellingShingle Izabelė Šuikaitė
Gabrielė Šiurkutė
Robert Ptacnik
Judita Koreivienė
Halotolerance of Phytoplankton and Invasion Success of Nostocalean Cyanobacteria Under Freshwater Salinization
Microorganisms
microbial invasion
cyanobacteria
halotolerance
alien species
harmful algae
disturbed ecosystems
title Halotolerance of Phytoplankton and Invasion Success of Nostocalean Cyanobacteria Under Freshwater Salinization
title_full Halotolerance of Phytoplankton and Invasion Success of Nostocalean Cyanobacteria Under Freshwater Salinization
title_fullStr Halotolerance of Phytoplankton and Invasion Success of Nostocalean Cyanobacteria Under Freshwater Salinization
title_full_unstemmed Halotolerance of Phytoplankton and Invasion Success of Nostocalean Cyanobacteria Under Freshwater Salinization
title_short Halotolerance of Phytoplankton and Invasion Success of Nostocalean Cyanobacteria Under Freshwater Salinization
title_sort halotolerance of phytoplankton and invasion success of nostocalean cyanobacteria under freshwater salinization
topic microbial invasion
cyanobacteria
halotolerance
alien species
harmful algae
disturbed ecosystems
url https://www.mdpi.com/2076-2607/13/6/1378
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