Isolation of lactic acid bacteria and quantification of Lentilactobacillus buchneri using qPCR in sorghum silage inoculated with native strains in tropical conditions
Abstract The objective of this study was to isolate lactic bacteria, quantify the population of Lentilactobacillus buchneri by qPCR, and evaluate the effects of the inoculation of native strains of L. buchneri in sorghum silage. The treatments were arranged in a 4 × 5 factorial scheme, with four ino...
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Academia Brasileira de Ciências
2025-03-01
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| author | JOÃO PAULO S. ROSEIRA ODILON G. PEREIRA TÂMARA C. DA SILVEIRA RICARDO M.A. PINHO RENAN S. CASCARDO ROSINEA A. DE PAULA POLIANE A. ZERBINI HILÁRIO C. MANTOVANI WAGNER S. ALVES KARINA G. RIBEIRO |
| author_facet | JOÃO PAULO S. ROSEIRA ODILON G. PEREIRA TÂMARA C. DA SILVEIRA RICARDO M.A. PINHO RENAN S. CASCARDO ROSINEA A. DE PAULA POLIANE A. ZERBINI HILÁRIO C. MANTOVANI WAGNER S. ALVES KARINA G. RIBEIRO |
| author_sort | JOÃO PAULO S. ROSEIRA |
| collection | DOAJ |
| description | Abstract The objective of this study was to isolate lactic bacteria, quantify the population of Lentilactobacillus buchneri by qPCR, and evaluate the effects of the inoculation of native strains of L. buchneri in sorghum silage. The treatments were arranged in a 4 × 5 factorial scheme, with four inoculants (I) and five fermentation periods (P) (7, 14, 28, 45, and 90), in a completely randomized design, with three replicates. Forty-seven LAB strains were isolated from control silage, and L. buchneri was the predominant species at 45 and 90 days of fermentation. The qPCR data showed that L. buchneri predominated during all fermentation periods in the inoculated silages. There was an effect (P<0.05) of I × P interaction on all studied variables of the fermentative profile and microbial population. There was an effect (P<0.05) of I × P interaction on the dry matter (DM), neutral detergent fiber (NDF), in vitro DM digestibility, and in vitro NDF digestibility. Inoculation with L. buchneri resulted in silages with better fermentative quality and digestibility and a lower yeast population. The native LB.1 and LB.4 strains have potential to be used as inoculants in sorghum silage production, with effects on fermentation quality at 45 days of storage. |
| format | Article |
| id | doaj-art-7ad945fecdc542de9a172bf0b67bebbd |
| institution | DOAJ |
| issn | 1678-2690 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Academia Brasileira de Ciências |
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| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj-art-7ad945fecdc542de9a172bf0b67bebbd2025-08-20T02:53:58ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902025-03-0197110.1590/0001-3765202520240783Isolation of lactic acid bacteria and quantification of Lentilactobacillus buchneri using qPCR in sorghum silage inoculated with native strains in tropical conditionsJOÃO PAULO S. ROSEIRAhttps://orcid.org/0000-0002-2321-6264ODILON G. PEREIRAhttps://orcid.org/0000-0002-1714-2959TÂMARA C. DA SILVEIRAhttps://orcid.org/0000-0001-7810-8642RICARDO M.A. PINHOhttps://orcid.org/0000-0002-2816-1610RENAN S. CASCARDOhttps://orcid.org/0000-0002-1216-8279ROSINEA A. DE PAULAhttps://orcid.org/0000-0002-4256-7473POLIANE A. ZERBINIhttps://orcid.org/0000-0002-8203-5244HILÁRIO C. MANTOVANIhttps://orcid.org/0000-0002-3481-5318WAGNER S. ALVEShttps://orcid.org/0000-0003-3161-5254KARINA G. RIBEIROhttps://orcid.org/0000-0002-1299-2807Abstract The objective of this study was to isolate lactic bacteria, quantify the population of Lentilactobacillus buchneri by qPCR, and evaluate the effects of the inoculation of native strains of L. buchneri in sorghum silage. The treatments were arranged in a 4 × 5 factorial scheme, with four inoculants (I) and five fermentation periods (P) (7, 14, 28, 45, and 90), in a completely randomized design, with three replicates. Forty-seven LAB strains were isolated from control silage, and L. buchneri was the predominant species at 45 and 90 days of fermentation. The qPCR data showed that L. buchneri predominated during all fermentation periods in the inoculated silages. There was an effect (P<0.05) of I × P interaction on all studied variables of the fermentative profile and microbial population. There was an effect (P<0.05) of I × P interaction on the dry matter (DM), neutral detergent fiber (NDF), in vitro DM digestibility, and in vitro NDF digestibility. Inoculation with L. buchneri resulted in silages with better fermentative quality and digestibility and a lower yeast population. The native LB.1 and LB.4 strains have potential to be used as inoculants in sorghum silage production, with effects on fermentation quality at 45 days of storage.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652025000101312&tlng=en16S rRNA genefermentationmolecular techniquetropical grass |
| spellingShingle | JOÃO PAULO S. ROSEIRA ODILON G. PEREIRA TÂMARA C. DA SILVEIRA RICARDO M.A. PINHO RENAN S. CASCARDO ROSINEA A. DE PAULA POLIANE A. ZERBINI HILÁRIO C. MANTOVANI WAGNER S. ALVES KARINA G. RIBEIRO Isolation of lactic acid bacteria and quantification of Lentilactobacillus buchneri using qPCR in sorghum silage inoculated with native strains in tropical conditions Anais da Academia Brasileira de Ciências 16S rRNA gene fermentation molecular technique tropical grass |
| title | Isolation of lactic acid bacteria and quantification of Lentilactobacillus buchneri using qPCR in sorghum silage inoculated with native strains in tropical conditions |
| title_full | Isolation of lactic acid bacteria and quantification of Lentilactobacillus buchneri using qPCR in sorghum silage inoculated with native strains in tropical conditions |
| title_fullStr | Isolation of lactic acid bacteria and quantification of Lentilactobacillus buchneri using qPCR in sorghum silage inoculated with native strains in tropical conditions |
| title_full_unstemmed | Isolation of lactic acid bacteria and quantification of Lentilactobacillus buchneri using qPCR in sorghum silage inoculated with native strains in tropical conditions |
| title_short | Isolation of lactic acid bacteria and quantification of Lentilactobacillus buchneri using qPCR in sorghum silage inoculated with native strains in tropical conditions |
| title_sort | isolation of lactic acid bacteria and quantification of lentilactobacillus buchneri using qpcr in sorghum silage inoculated with native strains in tropical conditions |
| topic | 16S rRNA gene fermentation molecular technique tropical grass |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652025000101312&tlng=en |
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