Antimicrobial and cytotoxic properties of calcium-enriched mixture cement, Iranian propolis, and propolis with herbal extracts in primary dental pulp stem cells
ObjectivesIn this study, natural substances were introduced as primary dental pulp caps for use in pulp therapy, and the antimicrobial and cytotoxic properties of these substances were investigated.Materials and MethodsIn this in vitro study, the antimicrobial properties of calcium-enriched mixture...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Korean Academy of Conservative Dentistry
2023-02-01
|
| Series: | Restorative Dentistry & Endodontics |
| Subjects: | |
| Online Access: | http://www.rde.ac/upload/pdf/rde-48-e2.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849426852865638400 |
|---|---|
| author | Mohammad Esmaeilzadeh Shirin Moradkhani Fahimeh Daneshyar Mohammad Reza Arabestani Sara Soleimani Asl Soudeh Tayebi Maryam Farhadian |
| author_facet | Mohammad Esmaeilzadeh Shirin Moradkhani Fahimeh Daneshyar Mohammad Reza Arabestani Sara Soleimani Asl Soudeh Tayebi Maryam Farhadian |
| author_sort | Mohammad Esmaeilzadeh |
| collection | DOAJ |
| description | ObjectivesIn this study, natural substances were introduced as primary dental pulp caps for use in pulp therapy, and the antimicrobial and cytotoxic properties of these substances were investigated.Materials and MethodsIn this in vitro study, the antimicrobial properties of calcium-enriched mixture (CEM) cement, propolis, and propolis individually combined with the extracts of several medicinal plants were investigated against Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Then, the cytotoxicity of each substance or mixture against pulp stem cells extracted from 30 primary healthy teeth was evaluated at 4 concentrations. Data were gathered via observation, and optical density values were obtained using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) test and recorded. SPSS software version 23 was used to analyze the data. Data were evaluated using 2-way analysis of variance and the Tukey test.ResultsRegarding antimicrobial properties, thyme alone and thyme + propolis had the lowest minimum inhibitory concentrations (MICs) against the growth of S. aureus, E. coli, and P. aeruginosa bacteria. For E. faecalis, thyme + propolis had the lowest MIC, followed by thyme alone. At 24 and 72 hours, thyme + propolis, CEM cement, and propolis had the greatest bioviability in the primary dental pulp stem cells, and lavender + propolis had the lowest bioviability.ConclusionsOf the studied materials, thyme + propolis showed the best results in the measures of practical performance as a dental pulp cap. |
| format | Article |
| id | doaj-art-7ad49bde9a5a4e698e647d139c092ec5 |
| institution | Kabale University |
| issn | 2234-7658 2234-7666 |
| language | English |
| publishDate | 2023-02-01 |
| publisher | Korean Academy of Conservative Dentistry |
| record_format | Article |
| series | Restorative Dentistry & Endodontics |
| spelling | doaj-art-7ad49bde9a5a4e698e647d139c092ec52025-08-20T03:29:14ZengKorean Academy of Conservative DentistryRestorative Dentistry & Endodontics2234-76582234-76662023-02-01481e210.5395/rde.2023.48.e21061Antimicrobial and cytotoxic properties of calcium-enriched mixture cement, Iranian propolis, and propolis with herbal extracts in primary dental pulp stem cellsMohammad Esmaeilzadeh0Shirin Moradkhani1Fahimeh Daneshyar2Mohammad Reza Arabestani3Sara Soleimani Asl4Soudeh Tayebi5Maryam Farhadian6Department of Pediatrics, Faculty of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, IranMedicinal Plants and Natural Products Research Center, Department of Pharmacognosy and Pharmaceutical Biotechnology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, IranDepartment of Pediatrics, Faculty of Dentistry, Hamadan University of Medical Sciences, Hamadan, IranDepartment of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IranEndometrium and Endometriosis Research Center, Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IranDepartment of Pediatrics, Faculty of Dentistry, Hamadan University of Medical Sciences, Hamadan, IranDepartment of Biostatistics, School of Public Health and Research Center for Health Sciences, Hamadan University of Medical Sciences, Hamadan, IranObjectivesIn this study, natural substances were introduced as primary dental pulp caps for use in pulp therapy, and the antimicrobial and cytotoxic properties of these substances were investigated.Materials and MethodsIn this in vitro study, the antimicrobial properties of calcium-enriched mixture (CEM) cement, propolis, and propolis individually combined with the extracts of several medicinal plants were investigated against Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Then, the cytotoxicity of each substance or mixture against pulp stem cells extracted from 30 primary healthy teeth was evaluated at 4 concentrations. Data were gathered via observation, and optical density values were obtained using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) test and recorded. SPSS software version 23 was used to analyze the data. Data were evaluated using 2-way analysis of variance and the Tukey test.ResultsRegarding antimicrobial properties, thyme alone and thyme + propolis had the lowest minimum inhibitory concentrations (MICs) against the growth of S. aureus, E. coli, and P. aeruginosa bacteria. For E. faecalis, thyme + propolis had the lowest MIC, followed by thyme alone. At 24 and 72 hours, thyme + propolis, CEM cement, and propolis had the greatest bioviability in the primary dental pulp stem cells, and lavender + propolis had the lowest bioviability.ConclusionsOf the studied materials, thyme + propolis showed the best results in the measures of practical performance as a dental pulp cap.http://www.rde.ac/upload/pdf/rde-48-e2.pdfcell viabilityherbalprimary teethpropolispulpotomystem cells |
| spellingShingle | Mohammad Esmaeilzadeh Shirin Moradkhani Fahimeh Daneshyar Mohammad Reza Arabestani Sara Soleimani Asl Soudeh Tayebi Maryam Farhadian Antimicrobial and cytotoxic properties of calcium-enriched mixture cement, Iranian propolis, and propolis with herbal extracts in primary dental pulp stem cells Restorative Dentistry & Endodontics cell viability herbal primary teeth propolis pulpotomy stem cells |
| title | Antimicrobial and cytotoxic properties of calcium-enriched mixture cement, Iranian propolis, and propolis with herbal extracts in primary dental pulp stem cells |
| title_full | Antimicrobial and cytotoxic properties of calcium-enriched mixture cement, Iranian propolis, and propolis with herbal extracts in primary dental pulp stem cells |
| title_fullStr | Antimicrobial and cytotoxic properties of calcium-enriched mixture cement, Iranian propolis, and propolis with herbal extracts in primary dental pulp stem cells |
| title_full_unstemmed | Antimicrobial and cytotoxic properties of calcium-enriched mixture cement, Iranian propolis, and propolis with herbal extracts in primary dental pulp stem cells |
| title_short | Antimicrobial and cytotoxic properties of calcium-enriched mixture cement, Iranian propolis, and propolis with herbal extracts in primary dental pulp stem cells |
| title_sort | antimicrobial and cytotoxic properties of calcium enriched mixture cement iranian propolis and propolis with herbal extracts in primary dental pulp stem cells |
| topic | cell viability herbal primary teeth propolis pulpotomy stem cells |
| url | http://www.rde.ac/upload/pdf/rde-48-e2.pdf |
| work_keys_str_mv | AT mohammadesmaeilzadeh antimicrobialandcytotoxicpropertiesofcalciumenrichedmixturecementiranianpropolisandpropoliswithherbalextractsinprimarydentalpulpstemcells AT shirinmoradkhani antimicrobialandcytotoxicpropertiesofcalciumenrichedmixturecementiranianpropolisandpropoliswithherbalextractsinprimarydentalpulpstemcells AT fahimehdaneshyar antimicrobialandcytotoxicpropertiesofcalciumenrichedmixturecementiranianpropolisandpropoliswithherbalextractsinprimarydentalpulpstemcells AT mohammadrezaarabestani antimicrobialandcytotoxicpropertiesofcalciumenrichedmixturecementiranianpropolisandpropoliswithherbalextractsinprimarydentalpulpstemcells AT sarasoleimaniasl antimicrobialandcytotoxicpropertiesofcalciumenrichedmixturecementiranianpropolisandpropoliswithherbalextractsinprimarydentalpulpstemcells AT soudehtayebi antimicrobialandcytotoxicpropertiesofcalciumenrichedmixturecementiranianpropolisandpropoliswithherbalextractsinprimarydentalpulpstemcells AT maryamfarhadian antimicrobialandcytotoxicpropertiesofcalciumenrichedmixturecementiranianpropolisandpropoliswithherbalextractsinprimarydentalpulpstemcells |