Carriage trends and fitness cost of MDROs in Lebanese nursing homes
Introduction: Nowadays, medical treatments efficiency is challenged by multi drug resistant organisms (MDROs). Lebanese nursing homes’ residents revealed high fecal carriage rates of MDR Enterobacteriacea. Previous studies claim that bacteria with resistant genes experience fitness cost. This study...
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The Journal of Infection in Developing Countries
2018-02-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/9996 |
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| author | Caren Challita Nourhane Hafza Elias Dahdouh Michel Attieh Iman Dandachi Ziad Daoud |
| author_facet | Caren Challita Nourhane Hafza Elias Dahdouh Michel Attieh Iman Dandachi Ziad Daoud |
| author_sort | Caren Challita |
| collection | DOAJ |
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Introduction: Nowadays, medical treatments efficiency is challenged by multi drug resistant organisms (MDROs). Lebanese nursing homes’ residents revealed high fecal carriage rates of MDR Enterobacteriacea. Previous studies claim that bacteria with resistant genes experience fitness cost. This study assesses the competitive growth of MDR Escherichia coli and Klebsiella pneumoniae.
Methodology: Fecal swabs were collected, during six consecutive months, from ten elderly residing in a Lebanese nursing home. All isolates were subject to API 20E (bioMerieux, Marcy L’ Etoile, France) and antimicrobial susceptibility (Kirby–Bauer method) testing. Phenotypically, ESBL (extended spectrum β-lactamase), MBL (metallo β-lactamase), AmpC and KPC (Klebsiella pneumoniae carbapenemase) were detected using EDTA, PBA, cloxacillin, and DDSTs (Biorad, Hercules, USA). Selected ESBL producing E. coli and K. pneumoniae underwent multiplex PCR analysis. Intra and inter-species in-vitro competition assays were conducted in multiple combinations.
Results: Among 117 collected isolates, E. coli was predominant (71.8%); 7.7% were ESBL and 5.1% AmpC producers. With E. coli intra-species assays, sensitive isolates out-competed all others, followed by ESBL, AmpC, and OXA-48 (oxacillin) producers. Inter-species assays, demonstrated a decreased fitness of ESBL producing K. pneumoniae in presence of sensitive E. coli. While out-competing ESBL producing E. coli required 2 sensitive K. pneumoniae isolates.
Conclusion: This study highlights resistant E. coli and K. pneumoniae frequency decrease in presence of sensitive isolates, endorsing the fitness cost hypothesis. Hence, competing supplementary species reproducing gut flora, would ensure further steps in the fight against MDROs.
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| format | Article |
| id | doaj-art-7ad2ca38d71b442793621565ff1db694 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2018-02-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-7ad2ca38d71b442793621565ff1db6942025-08-20T02:27:06ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802018-02-011202.110.3855/jidc.9996Carriage trends and fitness cost of MDROs in Lebanese nursing homesCaren Challita0Nourhane Hafza1Elias Dahdouh2Michel Attieh3Iman Dandachi4Ziad Daoud5Faculty of Medicine, Clinical Microbiology Lab, University of Balamand, LebanonFaculty of Sciences, University of Balamand, LebanonFaculty of Veterinary, Department of Animal Health, Complutense University of Madrid, Madrid, SpainFaculty of Medicine, Clinical Microbiology Lab, University of Balamand, LebanonFaculty of Medicine, Clinical Microbiology Lab, University of Balamand, LebanonFaculty of Medicine, Clinical Microbiology Lab, University of Balamand, Lebanon Introduction: Nowadays, medical treatments efficiency is challenged by multi drug resistant organisms (MDROs). Lebanese nursing homes’ residents revealed high fecal carriage rates of MDR Enterobacteriacea. Previous studies claim that bacteria with resistant genes experience fitness cost. This study assesses the competitive growth of MDR Escherichia coli and Klebsiella pneumoniae. Methodology: Fecal swabs were collected, during six consecutive months, from ten elderly residing in a Lebanese nursing home. All isolates were subject to API 20E (bioMerieux, Marcy L’ Etoile, France) and antimicrobial susceptibility (Kirby–Bauer method) testing. Phenotypically, ESBL (extended spectrum β-lactamase), MBL (metallo β-lactamase), AmpC and KPC (Klebsiella pneumoniae carbapenemase) were detected using EDTA, PBA, cloxacillin, and DDSTs (Biorad, Hercules, USA). Selected ESBL producing E. coli and K. pneumoniae underwent multiplex PCR analysis. Intra and inter-species in-vitro competition assays were conducted in multiple combinations. Results: Among 117 collected isolates, E. coli was predominant (71.8%); 7.7% were ESBL and 5.1% AmpC producers. With E. coli intra-species assays, sensitive isolates out-competed all others, followed by ESBL, AmpC, and OXA-48 (oxacillin) producers. Inter-species assays, demonstrated a decreased fitness of ESBL producing K. pneumoniae in presence of sensitive E. coli. While out-competing ESBL producing E. coli required 2 sensitive K. pneumoniae isolates. Conclusion: This study highlights resistant E. coli and K. pneumoniae frequency decrease in presence of sensitive isolates, endorsing the fitness cost hypothesis. Hence, competing supplementary species reproducing gut flora, would ensure further steps in the fight against MDROs. https://jidc.org/index.php/journal/article/view/9996fecal carriagefitness costmultidrug-resistantnursing home |
| spellingShingle | Caren Challita Nourhane Hafza Elias Dahdouh Michel Attieh Iman Dandachi Ziad Daoud Carriage trends and fitness cost of MDROs in Lebanese nursing homes Journal of Infection in Developing Countries fecal carriage fitness cost multidrug-resistant nursing home |
| title | Carriage trends and fitness cost of MDROs in Lebanese nursing homes |
| title_full | Carriage trends and fitness cost of MDROs in Lebanese nursing homes |
| title_fullStr | Carriage trends and fitness cost of MDROs in Lebanese nursing homes |
| title_full_unstemmed | Carriage trends and fitness cost of MDROs in Lebanese nursing homes |
| title_short | Carriage trends and fitness cost of MDROs in Lebanese nursing homes |
| title_sort | carriage trends and fitness cost of mdros in lebanese nursing homes |
| topic | fecal carriage fitness cost multidrug-resistant nursing home |
| url | https://jidc.org/index.php/journal/article/view/9996 |
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