Investigation of antibacterial compounds from actinomycetes against wastewater associated bacteria
Freshwater scarcity is an escalating global issue, requiring innovative approaches to ensure water quality and sustainability. This study aimed to isolate actinomycetes from soil and wastewater environments that have effectiveness in removing bacteria associated with wastewater. A total of 25 actino...
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| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Water Science |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/23570008.2025.2510040 |
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| author | Karam R. Wafy |
| author_facet | Karam R. Wafy |
| author_sort | Karam R. Wafy |
| collection | DOAJ |
| description | Freshwater scarcity is an escalating global issue, requiring innovative approaches to ensure water quality and sustainability. This study aimed to isolate actinomycetes from soil and wastewater environments that have effectiveness in removing bacteria associated with wastewater. A total of 25 actinomycetes isolates were obtained from the collected soil and wastewater samples for further analysis. These isolates will be evaluated for their potential to act as biocontrol agents against wastewater-associated bacteria, highlighting their role in enhancing wastewater treatment processes. Actinomycetes isolates were screened for antibacterial activity using the cross-streak method, and the most effective isolate (KW8) was further analyzed. Antibacterial compounds were extracted using ethyl acetate and characterized via gas chromatography-mass spectrometry (GC-MS), library data identified 13 bioactive compounds: 2-methyl-7-nonadecene, 10-heneicosene, n-hexadecanoic acid, cetene, 1-docosene, trans-geranylgeraniol, [1,1”-biphenyl]-4,4”-diol, 3,3“,5,5”-tetrakis(1,1-dimethylethyl), pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(phenylmethyl), 1-nonadecene, trichloroacetic acid, hexadecyl ester, 1-eicosanol, squalene, and dodecane, 1,2-dibromo. KW8 exhibited broad-spectrum antibacterial activity, with the highest inhibition observed against Enterococcus faecalis (20 ± 1.3 mm) and the lowest against Pseudomonas aeruginosa (12 ± 0.9 mm). The strain was identified through 16S rRNA sequencing and confirmed as Streptomyces gramineus. These findings highlight KW8’s potential as an effective antimicrobial agent for wastewater treatment, providing a sustainable solution to address microbial contamination and promoting environmental health. |
| format | Article |
| id | doaj-art-7ace2b8e268a49d7a2a79b00caca3812 |
| institution | Kabale University |
| issn | 2357-0008 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Water Science |
| spelling | doaj-art-7ace2b8e268a49d7a2a79b00caca38122025-08-20T03:28:05ZengTaylor & Francis GroupWater Science2357-00082025-12-0139137137910.1080/23570008.2025.2510040Investigation of antibacterial compounds from actinomycetes against wastewater associated bacteriaKaram R. Wafy0Microbiology Department, Central Laboratory for Environmental Quality Monitoring (CLEQM), National Water Research Center (NWRC), Cairo, EgyptFreshwater scarcity is an escalating global issue, requiring innovative approaches to ensure water quality and sustainability. This study aimed to isolate actinomycetes from soil and wastewater environments that have effectiveness in removing bacteria associated with wastewater. A total of 25 actinomycetes isolates were obtained from the collected soil and wastewater samples for further analysis. These isolates will be evaluated for their potential to act as biocontrol agents against wastewater-associated bacteria, highlighting their role in enhancing wastewater treatment processes. Actinomycetes isolates were screened for antibacterial activity using the cross-streak method, and the most effective isolate (KW8) was further analyzed. Antibacterial compounds were extracted using ethyl acetate and characterized via gas chromatography-mass spectrometry (GC-MS), library data identified 13 bioactive compounds: 2-methyl-7-nonadecene, 10-heneicosene, n-hexadecanoic acid, cetene, 1-docosene, trans-geranylgeraniol, [1,1”-biphenyl]-4,4”-diol, 3,3“,5,5”-tetrakis(1,1-dimethylethyl), pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(phenylmethyl), 1-nonadecene, trichloroacetic acid, hexadecyl ester, 1-eicosanol, squalene, and dodecane, 1,2-dibromo. KW8 exhibited broad-spectrum antibacterial activity, with the highest inhibition observed against Enterococcus faecalis (20 ± 1.3 mm) and the lowest against Pseudomonas aeruginosa (12 ± 0.9 mm). The strain was identified through 16S rRNA sequencing and confirmed as Streptomyces gramineus. These findings highlight KW8’s potential as an effective antimicrobial agent for wastewater treatment, providing a sustainable solution to address microbial contamination and promoting environmental health.https://www.tandfonline.com/doi/10.1080/23570008.2025.2510040Actinomycetesan antagonistic activitywastewater treatmentcross-streak method |
| spellingShingle | Karam R. Wafy Investigation of antibacterial compounds from actinomycetes against wastewater associated bacteria Water Science Actinomycetes an antagonistic activity wastewater treatment cross-streak method |
| title | Investigation of antibacterial compounds from actinomycetes against wastewater associated bacteria |
| title_full | Investigation of antibacterial compounds from actinomycetes against wastewater associated bacteria |
| title_fullStr | Investigation of antibacterial compounds from actinomycetes against wastewater associated bacteria |
| title_full_unstemmed | Investigation of antibacterial compounds from actinomycetes against wastewater associated bacteria |
| title_short | Investigation of antibacterial compounds from actinomycetes against wastewater associated bacteria |
| title_sort | investigation of antibacterial compounds from actinomycetes against wastewater associated bacteria |
| topic | Actinomycetes an antagonistic activity wastewater treatment cross-streak method |
| url | https://www.tandfonline.com/doi/10.1080/23570008.2025.2510040 |
| work_keys_str_mv | AT karamrwafy investigationofantibacterialcompoundsfromactinomycetesagainstwastewaterassociatedbacteria |